The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 21 , ISSUE 4 ( April, 2020 ) > List of Articles


Evaluation with Micro-CT of the Canal Seal Made with Two Different Bioceramic Cements: Guttaflow Bioseal and Bioroot RCS

Edoardo Bianco, Chiara Calvelli, Claudio L Citterio, Alberto Pellegatta, Pier M Venino

Keywords : Abfraction, Air void, Bioceramic, Endodontic cement, Gutta-percha, Laboratory research, Micro-CT, Occlusion

Citation Information : Bianco E, Calvelli C, Citterio CL, Pellegatta A, Venino PM. Evaluation with Micro-CT of the Canal Seal Made with Two Different Bioceramic Cements: Guttaflow Bioseal and Bioroot RCS. J Contemp Dent Pract 2020; 21 (4):359-366.

DOI: 10.5005/jp-journals-10024-2816

License: CC BY-NC 4.0

Published Online: 25-02-2012

Copyright Statement:  Copyright © 2020; The Author(s).


Aim: The aim of this work is to investigate the quality of root canal seals obtained by comparing two bioceramic cements, GuttaFlow bioseal and BioRoot RCS, focusing on the presence of voids created during the canal obturation procedure. Materials and methods: The voids are analyzed using a micro-computed tomography (micro-CT) device. The study will be performed using images of the endodontic space before and after filling of a selected group of elements. Furthermore, the average thickness of the cement, the average quantity of gutta-percha compared to the total shaped volume, and the average quantity of the two cements, GuttaFlow bioseal and BioRoot RCS, with respect to the total shaped volume were considered. The apical, middle, and coronal thirds will also be investigated in a sectorial manner. Images have been analyzed using a CT-An™ software and visualized through a three-dimensional (3D) reconstruction of the slices by the software CT-Vol™. Shapiro–Wilk test/Test D’Agostino-Pearson/Kolmogorov–Smirnov test were used to ensure the reliability of results. Results: No significant differences were observed in the amount of gutta-percha compared to the shaped volume between the GuttaFlow bioseal group and BioRoot RCS. No statistically significant difference was observed between the two groups in terms of voids. Conclusion: The data obtained from this study allowed to conclude that the samples filled with GuttaFlow bioseal and BioRoot RCS have a similar seal capacity since no statistically significant differences were observed between the two groups. No sample showed the absence of voids within the root canal obturation. Clinical significance: Even if the two cements tested showed differences in terms of void volume and ability to fill thin spaces, they should be considered both acceptable and equivalent in terms of clinical sealing ability.

  1. Song M, Kim HC, Lee W, et al. Analysis of the cause of failure in nonsurgical endodontic treatment by microscopic inspection during endodontic microsurgery. Endod 2011;37(11):1516–1519. DOI: 10.1016/j.joen.2011.06.032.
  2. Venino PM, Citterio CL, Pellegatta A, et al. A micro-computed tomography evaluation of the shaping ability of two nickel-titanium instruments, Hyflex EDM and protaper next. J Endod 2017;43(4): 628–632. DOI: 10.1016/j.joen.2016.11.022.
  3. Tuncer S, Demirci M, Tekçe N, et al. The effect of two bulk fill resin composites on microleakage in endodontically treated teeth. J Dentists 2013;1(1):8–15. DOI: 10.12974/2311-8695.2013.01.01.2.
  4. Scavia S, Roncucci R, Bianco E, et al. Minimal invasive flapless piezotome alveolar crest horizontal split technique: preliminary results. J Contemp Dent Pract 2020;21(1):28–35. DOI: 10.5005/jp-journals-10024-2743.
  5. Bianco E, Rota D. Oral findings in Rett syndrome: an update and review of the literature. Dent Med Probl 2018;55(4):441–445. DOI: 10.17219/dmp/99203.
  6. Citterio F, Pellegatta A, Citterio CL, et al. Analysis of the apical constriction using micro-computed tomography and anatomical sections. Giornale Italiano Di Endodonzia 2014;28(1):41–45. DOI: 10.1016/j.gien.2014.05.001.
  7. Maddalone M, Gagliani M, Citterio CL, et al. Prevalence of vertical root fractures in teeth planned for apical surgery. A retrospective cohort study. Int Endod J 2018;51(9):969–974. DOI: 10.1111/iej.12910.
  8. Ambu E, Citterio CL, Pellegatta A, et al. The use of limited CBCT in the early diagnosis of root vertical fracture: a case report. Glob J Oral Sci 2018;4(1):18–24. DOI: 10.30576/2414-2050.2018.04.4.
  9. Brownlee WA. Filling of root canals in recently devitalized teeth. Dominion Dent J 1900;12(8):254.
  10. Grossman LI. An improved root canal cement. JADA 1958;56(3):381–385. DOI: 10.14219/jada.archive.1958.0055.
  11. Wu MK, De Gee AJ, Wesselink PR. Leakage of four root canal sealers at different thickness. Int Endod J 1994;27(6):304–308. DOI: 10.1111/j.1365-2591.1994.tb00273.x.
  12. Weis MV, Parashos P, Messer HH. Effect of obturation technique on sealer cement thickness and dentinal tubule penetration. Int Endod J 2004;37(10):653–663. DOI: 10.1111/j.1365-2591.2004.00839.x.
  13. Viapiana R, Flumignan DL, Guerreiro-Tanomaru JM, et al. Physicochemical and mechanical properties of zirconium oxide and niobiumoxide modified portland cement-based experimental endodontic sealers. Int Endod J 2014;47(5):437–448. DOI: 10.1111/iej.12167.
  14. AL-Haddad A, Ab Aziz C, Zeti A. Review article bioceramic-based root canal sealers: a review. Int J Biomater 2016;2016:9753210. DOI: 10.1155/2016/9753210.
  15. Bianco E, Maddalone M, Porcaro G, et al. Treatment of osteoradionecrosis of the jaw with ozone in the form of oil-based gel: 1-year follow-up. J Contemp Dent Pract 2019;20(2):270–276. DOI: 10.5005/jp-journals-10024-2508.
  16. Michaud RA, Burgess J, Barfield RD, et al. Volumetric expansion of gutta-percha in contact with eugenol. J Endod 2008;34(12):1528–1532. DOI: 10.1016/j.joen.2008.08.025.
  17. Ozok AR, van der Sluis LW, Wu MK, et al. Sealing ability of a new polydimethylsiloxane-based root canal filling material. J Endod 2008;34(2):204–207. DOI: 10.1016/j.joen.2007.11.005.
  18. Sönmez IS, Oba AA, Sönmez D, et al. In vitro evaluation of apical microleakage of a new MTA-based sealer. Eur Arch Paediatr Dent 2012;13(5):252–255. DOI: 10.1007/bf03262880.
  19. Generali L, Prati C, Pirani C, et al. Gandolfi MG.double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer. Clin Oral Investig 2017;21(4):1267–1276. DOI: 10.1007/s00784-016-1878-0.
  20. Celikten B, Uzuntas CF, Orhan AI, et al. Evaluation of root canal sealer filling quality using a single-cone technique in oval shaped canals: an in vitro micro-CT study. Scanning 2016;38(2):133–140. DOI: 10.1002/sca.21249.
  21. Porcaro G, Busa A, Bianco E, et al. Use of a partial-thickness flap for guided bone regeneration in the upper jaw. J Contemp Dent Pract 2017;18(12):1117–1121. DOI: 10.5005/jp-journals-10024-2186.
  22. Bianco E, Attuati S, Brugali C, et al. Restoration of the vertical posterior dimension in a grinding patient before orthodontic treatment: a casereport with electromyographic evaluation of masticatory muscles balance. J Dent 2019;7:38–42. DOI:
  23. Galluzzi F, Pignataro L, Maddalone M, et al. Recurrences of surgery for antrochoanal polyps in children: a systematic review. Int J Pediatr Otorhinolaryngol 2018;106:26–30. DOI: 10.1016/j.ijporl.2017. 12.035.
  24. Saati S, Eskandarloo A, Falahi A, et al. Evaluation of the efficacy of the metal artifact reduction algorithm in the detection of a vertical root fracture in endodontically treated teeth in cone-beam computed tomography images: an in vitro study. Dent Med Probl 2019;56(4):357–363. DOI: 10.17219/dmp/109902.
  25. Krastev BP. Laser apicoectomy with ER:YAG and bone xenograft bio-Oss Collagen®. A case report. The Journal of Dentists 2014;2(2):90–95. DOI:
  26. Grout A, Speakman EM. In-flight transmission of foodborne disease: How can airlines improve? Travel Med Infect Dis 2020;33:101558. DOI: 10.1016/j.tmaid.2020.101558.
  27. Maddalone M, Bianco E, Nanussi A, et al. Treatment of temporomandibular disorders of muscular origin with a silicon oral device (Alifix®): electromyographic analysis. J Contemp Dent Pract 2019;20(12):1367–1374. DOI: 10.5005/jp-journals-10024-2704.
  28. Bianco E. Factors influencing immediate maxillary dental implant placement and bone resorption: a review of the literature and an outlook on the clinical possibilities. Dent Med Probl 2016;53(3):408–412. DOI: 10.17219/dmp/63185.
  29. Silva EJNL, Canabarro A, Andrade MRTC, et al. Dislodgment resistance of bioceramic and epoxy sealers: a systematic review and metaanalysis. J Evid Based Dent Pract 2019;19(3):221–235. DOI: 10.1016/j. jebdp.2019.04.004.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.