Aim: The aim of this survey was to evaluate the ability of dental students and interns to deliver oral hygiene instructions. Materials and methods: This survey targeted patients aged 17–67 years who sought different dental treatments performed by final (sixth)-year dental students or dental interns. Both patients and therapists participated in filling out the questionnaire. The questionnaire included an exploration of the ability of therapists to prescribe dental hygiene aids commonly used in oral hygiene dentistry. Results: In total, 150 patients and 150 therapists of both genders participated in this survey. The results showed that 47.3% of all therapists taught the patients the brushing technique; however, only 20% of the therapists reinforced oral hygiene instructions. Similarly, only 26% of therapists instructed their patients to renew their toothbrushes regularly, and 34% of the therapists instructed the patients on using dental floss. Only 32% of therapists prescribed a mouthwash to their patients, and 40.7% of the therapists used a disclosing agent. The difference was highly significant between students and interns in all the above activities, in favor of the dental students. Conclusion: The result shows a low level of therapist's performance, both students and interns, in delivering oral hygiene instructions to their patients. Clinical significance: Dental students and interns take a big responsibility in motivation and delivery of oral hygiene instructions in dental clinics to their patients. Evaluate the performance of students and interns in delivering oral health instructions in dental clinics will facilitate establish oral health programs to ensure improve the performance of therapists in motivation and delivering the oral hygiene instructions and therefore increase the ability of patients to respond.