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VOLUME 21 , ISSUE 7 ( July, 2020 ) > List of Articles


Evaluation of the Antibacterial and Antifungal Effects of ProRoot MTA and Nano-fast Cement: An In Vitro Study

Fariborz Moazami, Ahmad Gholami, Vahid Mehrabi, Yasamin Ghahramani

Citation Information : Moazami F, Gholami A, Mehrabi V, Ghahramani Y. Evaluation of the Antibacterial and Antifungal Effects of ProRoot MTA and Nano-fast Cement: An In Vitro Study. J Contemp Dent Pract 2020; 21 (7):760-764.

DOI: 10.5005/jp-journals-10024-2877

License: CC BY-NC 4.0

Published Online: 19-08-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Aim: One of the most vital characteristics of an ideal root filling material is the capability to inhibit the growth of the microorganisms. Mineral trioxide aggregate (MTA) is one of the most used root repair materials, with approved antibacterial effect. A newly introduced root repair material is nano-fast cement (NFC) which should be investigated. The antibacterial and antifungal activities of NFC were evaluated in the present study. Materials and methods: Enterococcus faecalis (PTCC 1394), Escherichia coli (ATTC 15224), and Candida albicans (PTCC 5027) were employed for the antimicrobial assessment. The following were the steps used to conduct the agar diffusion test (ADT): six agar plates were used. 0.5 McFarland concentration of each strain was cultured on two plates by a sterile cotton-tipped swab. Three holes with 5mm diameter were created on each plate. Freshly mixed cement was placed in the holes of the related plate. After two hours, the plates were incubated at 37°C for 24 hours. Then, the diameter of the growth inhibition zones were measured, and the mean values were used for the analysis. Direct contact test (DCT) was done by using the following steps: Freshly mixed materials were placed in the 96-well microtiter plate. 10 μL of each bacterial suspension was added to the tested cement. After one-hour incubation at 37°C, 245 μL of BHI broth was added to each well, and the plate was vortexed for 2 minutes. About 15 μL of this bacterial suspension was added to a new well which contained 215 μL of fresh medium. The kinetics of the bacterial outgrowth were measured by the microplate spectrophotometer hourly for 12 hours. Results: No significant differences were observed between the diameters of the growth inhibition zones of MTA and NFC groups in ADT. In DCT, the MTA inhibits E. coli more effectively than NFC (p value < 0.001). Both cements had the same inhibitory effect on E. faecalis and C. albicans. Conclusion: The MTA and NFC are almost equally effective against the tested microorganisms. Clinical significance: The antibacterial characteristic of any dental material is an important matter. As well, the antibacterial efficacy of the NFC should be evaluated.

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  1. Fabricious L, Dahlen G, Öhman AE, et al. Predominant indigenous oral bacteria isolated from infected root canals after varied times of closure. Eur J Oral Sci 1982;2(90):134–144. DOI: 10.1111/j.1600-0722.1982.tb01536.x.
  2. Fouad AF, Zerella J, Barry J, et al. Molecular detection of enterococcus species in root canals of therapy-resistant endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol 2005;1(99):112–118. DOI: 10.1016/j.tripleo.2004.06.064.
  3. Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review—part II: leakage and biocompatibility investigations. J Endod 2010;2(36):190–202. DOI: 10.1016/j.joen.2009.09.010.
  4. Baumgartner JC, Siqueira JF, Sedgley CM, et al. Ingle's endodontics, 6th ed. 2008(2):221–308. DOI: 10.1097/SAP.0b013e31815f39dc.
  5. Torabinejad M, Hong CU, Ford TRP, et al. Antibacterial effects of some root end filling materials. J Endod 1995;8(21):403–406. DOI: 10.1016/s0099-2399(06)80824-1.
  6. Witherspoon DE, Small JC, Harris GZ. Mineral trioxide aggregate pulpotomies: a case series outcomes assessment. J Am Dent Assoc 2006;5(137):610–618. DOI: 10.14219/jada.archive.2006.0256.
  7. El Meligy OA, Avery DR. Comparison of apexification with mineral trioxide aggregate and calcium hydroxide. J Pediatr Dent 2006;3(28):248–253.
  8. Bogen G, Kuttler S. Mineral trioxide aggregate obturation: a review and case series. J Endod 2009;6(35):777–790. DOI: 10.1016/j.joen.2009.03.006.
  9. Al-Hezaimi K, Al-Shalan TA, Naghshbandi J, et al. Antibacterial effect of two mineral trioxide aggregate (MTA) preparations against enterococcus faecalis and streptococcus sanguis in vitro. J Endod 2006;11(32):1053–1056. DOI: 10.1016/j.joen.2006.06.004.
  10. Stowe T, Sedgley C, Stowe B, et al. The effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored ProRoot mineral trioxide aggregate. J Endod 2004;6(30):429–431. DOI: 10.1097/00004770-200406000-00013.
  11. Sipert CR, Hussne RP, Nishiyama CK, et al. In vitro antimicrobial activity of fill canal, sealapex, mineral trioxide aggregate, Portland cement and endorez. Int Endod 2005;8(38):539–543. DOI: 10.1111/j.1365-2591.2005.00984.x.
  12. Bhavana V, Chaitanya K, Dola B, et al. Evaluation of antibacterial and antifungal activity of new calcium-based cement (biodentine) compared to MTA and glass ionomer cement. J Conserv Dent 2015;1(18):44. DOI: 10.4103/0972-0707.148892.
  13. Al-Nazhan S, Al-Judai A. Evaluation of antifungal activity of mineral trioxide aggregate. J Endod 2003;12(29):826–827. DOI: 10.1097/00004770-200312000-00010.
  14. Koh ET, McDonald F, Pitt Ford TR, et al. Cellular response to mineral trioxide aggregate. J Endod 1998;8(24):543–547. DOI: 10.1016/S0099-2399(98)80074-5.
  15. Zhu Q, Haglund R, Safavi K, et al. Adhesion of human osteoblasts on root-end filling materials. J Endod 2000;7(26):404–406. DOI: 10.1097/00004770-200007000-00006.
  16. Lee S-J, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 1993;11(19):541–544. DOI: 10.1016/S0099-2399(06)81282-3.
  17. Aqrabawi J. Sealing ability of amalgam, super EBA cement, and MTA when used as retrograde filling materials. Br Dent J 2000;5(188): 266–268. DOI: 10.1038/sj.bdj.4800450.
  18. Keiser K, Johnson CC, Tipton DA. Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts. J Endod 2000;5(26):288–291. DOI: 10.1097/00004770-200005000-00010.
  19. De Deus G, Ximenes R, Gurgel-Filho ED, et al. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod 2005;9(38):604–609. DOI: 10.1111/j.1365-2591.2005.00987.x.
  20. Maeda H, Nakano T, Tomokiyo A, et al. Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells. J Endod 2010;4(36):647–652. DOI: 10.1016/j.joen.2009.12.024.
  21. Krastl G, Allgayer N, Lenherr P, et al. Tooth discoloration induced by endodontic materials: a literature review. Dent Traumatol 2013;1(29):2–7. DOI: 10.1111/j.1600-9657.2012.01141.x.
  22. Johnson BR. Considerations in the selection of a root-end filling material. Oral Surg Oral Med Oral Pathol Oral Radiol 1999;4(87):398–404. DOI: 10.1016/s1079-2104(99)70237-4.
  23. Sanaee MR, Danesh Manesh H, Janghorban K, et al. The influence of particle size and multi-walled carbon nanotube on physical properties of mineral trioxide aggregate. Mater Res 2019;6(6):065413. DOI: 10.1088/2053-1591/ab0f54.
  24. Weiss E, Shalhav M, Fuss Z. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Dent Traumatol 1996;4(12):179–184. DOI: 10.1111/j.1600-9657.1996.tb00511.x.
  25. Sundqvist G. Ecology of the root canal flora. J Endod 1992;9(18):427–430. DOI: 10.1016/S0099-2399(06)80842-3.
  26. Stuart C, Schwartz S, Beeson T, et al. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006;2(32):93–98. DOI: 10.1016/j.joen.2005.10.049.
  27. Hirasawa M, Takada K. Multiple effects of green tea catechin on the antifungal activity of antimycotics against Candida albicans. J Antimicrob 2004;2(53):225–229. DOI: 10.1093/jac/dkh046.
  28. Nair PNR, Sjögren U, Krey G, et al. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod 1990;12(16):580–588. DOI: 10.1016/S0099-2399(07)80201-9.
  29. Siqueira JF, Rocas IN, Lopes HP, et al. Fungal infection of the radicular dentin. J Endod 2002;11(28):770–773. DOI: 10.1097/00004770-200211000-00006.
  30. Sen B, Piskin B, Demirci T. Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM. Dent Traumatol 1995;1(11):6–9. DOI: 10.1111/j.1600-9657.1995.tb00671.x.
  31. Waltimo TMT, Orstavik D, Siren EK, et al. In vitro susceptibility of Candida albicans to four disinfectants and their combinations. Int Endod 1999;6(32):421–429. DOI: 10.1046/j.1365-2591.1999.00237.x.
  32. Heling I, Chandler N. Antimicrobial effect of irrigant combinations within dentinal tubules. Int Endod 1998;1(31):8–14. DOI: 10.1046/j.1365-2591.1998.t01-1-00124.x.
  33. Leonardo M, Da Silva L, Filho M, et al. In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics. J Endod 2000;7(26):391–394. DOI: 10.1097/00004770-200007000-00003.
  34. Çobankara FK, Altinöz H, Erganiş O, et al. In vitro antibacterial activities of root-canal sealers by using two different methods. J Endod 2004;1(30):57–60. DOI: 10.1097/00004770-200401000-00013.
  35. Estrela C, Sydney GB, Bammann LL, et al. Mechanism of the action of calcium and hydroxy ions of calcium hydroxide on tissue and bacteria. Braz Dent J 1995;6(2):85–90.
  36. Duarte MAH, Demarchi ACCO, Yamashita JC, et al. pH and calcium ion release of 2 root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol 2003;3(95):345–347. DOI: 10.1067/moe. 2003.12.
  37. Eldeniz AU, Hadimli HH, Ataoglu H, et al. Antibacterial effect of selected root-end filling materials. J Endod 2006;4(32):345–349. DOI: 10.1016/j.joen.2005.09.009.
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