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VOLUME 21 , ISSUE 8 ( August, 2020 ) > List of Articles

ORIGINAL RESEARCH

Comparative Evaluation of Antifungal Activity of Octenidine: An In Vitro Confocal Laser Study

Dinesh Sridhar, Arasappan Rajkumar, Karthikeyan Selvaraj, Sathish Sankar

Keywords : Antifungal activity, Candida albicans, Confocal laser scanning microscope, Endodontic irrigants

Citation Information : Sridhar D, Rajkumar A, Selvaraj K, Sankar S. Comparative Evaluation of Antifungal Activity of Octenidine: An In Vitro Confocal Laser Study. J Contemp Dent Pract 2020; 21 (8):905-909.

DOI: 10.5005/jp-journals-10024-2860

License: CC BY-NC 4.0

Published Online: 30-09-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: The aim of this study was to evaluate the antifungal efficacy of a novel endodontic irrigant octenidine against conventional irrigants sodium hypochlorite and EDTA on Candida albicans growth in the young and old population by calculating the number of colonies formed and by qualitative identification of dead/viable fungi by Confocal laser scanning microscopy (CLSM) method. Materials and methods: The total number of samples used in the study was eighty samples after decoronation of the crown portion the middle third of root canal. Each group was subdivided into four subgroups with various irrigation protocols: (A) 17% EDTA + 5.25% NaOCl, (B) 100% Octenisept, (C) 17% EDTA + 5.25% NaOCl + 1% clotrimazole, and (D) phosphate buffer saline. After completion of irrigation ATCC samples (90028) of C. albicans were inoculated with 5 mL of peptone water each and incubated at 37°C for 72 hours to attain the turbidity corresponding to 0.5 McFarland standards CFU. Eight samples were analyzed for the formation of candidal colonies, and two samples for the assessment of viability of Candida by confocal laser scanning microscope in each subgroup. Results: Comparison of antifungal efficacy of endodontic irrigants employed in the young and old populations revealed a significant reduction in the mean values of CFU and the mean values of percentage of non-viable microorganism by CLSM method. A positive relationship was revealed in the younger population which had a better antifungal efficacy than the older population in all the irrigant subgroups evaluated in the study. Conclusion: All the endodontic irrigants employed in our study had a good antifungal efficacy against Candida albicans. Octenisept had a maximum antifungal efficacy, while phosphate saline showed the least efficacy in both age groups, which was quantitatively evaluated by CFU method, and the same was confirmed through qualitative evaluation by CLSM method. Clinical significance: Candida albicans plays a major role in the establishment and pathogenesis of failed root canal treatment. Age-related changes alter the adhesion potential of dentin, in turn influence the outcome of endodontic therapy. Octenidine, a novel antifungal agent, can be substituted over the conventionally used EDTA and NaOCl with less adverse effects.


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  1. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surgery, Oral Medicine, Oral Pathology 1965;20(3):340–349. DOI: 10.1016/0030-4220(65)90166-0.
  2. Waltimo TM, Orstavik D, Sirén EK, et al. In vitro yeast infection of human dentin. J Endod 2000;26(4):207–209. DOI: 10.1097/00004770-200004000-00002.
  3. Siqueira JF, Rocas IN, Lopes HP, et al. Fungal infection of the radicular dentin. J Endod 2002;28(11):770–773. DOI: 10.1097/00004770-200211000-00006.
  4. Brändle N, Zehnder M, Weiger R, et al. Impact of growth conditions on susceptibility of five microbial species to alkaline stress. J Endod 2008;34(5):579–582. DOI: 10.1016/j.joen.2008.02.027.
  5. Şen BH, Safavi KE, Spangberg LS. Growth patterns of Candida albicans in relation to radicular dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997;84(1):68–73.
  6. Şen BH, Safavi KE, Spangberg LS. Antifungal effects of sodium hypochlorite and chlorhexidine in root canals. J Endod 1999;25(4): 235–238. DOI: 10.1016/S0099-2399(99)80149-6.
  7. Sena NT, Gomes BPFA, Vianna ME, et al. In vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species bio films. Int Endod J 2006;39(11):878–885. DOI: 10.1111/j.1365-2591.2006.01161.x.
  8. Ozdemir HO, Buzoglu HD, Calt S, et al. Effect of ethylenediamine-tetraacetic acid and sodium hypochlorite irrigation on Enterococcus faecalis biofilm colonization in young and old human root canal dentin: in vitro study. J Endod 2010;36(5):842–846. DOI: 10.1016/j.joen.2010.01.008.
  9. Chandra SS, Miglani R, Srinivasan MR, et al. Antifungal efficacy of 5.25% sodium hypochlorite, 2% chlorhexidine gluconate, and 17% EDTA with and without an antifungal agent. J Endod 2010;36(4): 675–678. DOI: 10.1016/j.joen.2010.01.015.
  10. Tandjung L, Waltimo T, Hauser I, et al. Octenidine in root canal and dentine disinfection ex vivo. Int Endod J 2007;40(11):845–851. DOI: 10.1111/j.1365-2591.2007.01279.x.
  11. Kakoli P, Nandakumar R, Romberg E, et al. The effect of age on bacterial penetration of radicular dentin. J Endod 2009;35(1):78–81. DOI: 10.1016/j.joen.2008.10.004.
  12. Baumgartner JC, Watts CM, Xia T. Occurrence of Candida albicans in infections of endodontic origin. J Endod 2000;26(12):695–698. DOI: 10.1097/00004770-200012000-00003.
  13. Venegas SC, Palacios JM, Apella MC, et al. Calcium modulates interactions between bacteria and hydroxyapatite. J Dent Res 2006;85(12):1124–1128. DOI: 10.1177/154405910608501211.
  14. Kinney JH, Nalla RK, Pople JA, et al. Age-related transparent root dentin: mineral concentration, crystallite size, and mechanical properties. Biomaterials 2005;26(16):3363–3376. DOI: 10.1016/j.biomaterials.2004.09.004.
  15. Radcliffe CE, Potouridou L, Qureshi R, et al. Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontic microorganisms actinomyces Israeli, A. Naeslundii, Candida albicans and Enterococcus faecalis. Int Endod J 2004;37(7):438–446. DOI: 10.1111/j.1365-2591.2004.00752.x.
  16. Ates M, Akdeniz BG, Sen BH. The effect of calcium chelating or binding agents on Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005;100(5):626–630. DOI: 10.1016/j.tripleo.2005.03.004.
  17. Jin Y, Zhang T, Samaranayake YH, et al. The use of new probes and stains for improved assessment of cell viability and extracellular polymeric substances in Candida albicans biofilm. Mycopathologia 2005;159(3):353–360. DOI: 10.1007/s11046-004-6987-7.
  18. Fidalgo TK, Barcelos R, Portela MB, et al. Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus. Braz Oral Res 2010;24(4):406–412. DOI: 10.1590/s1806-83242010000400006.
  19. Tirali RE, Turan Y, Akal N, et al. In vitro antimicrobial activity of several concentrations of NaOCl and octenisept in elimination of endodontic pathogens. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108(5):e117–e120. DOI: 10.1016/j.tripleo.2009. 07.012.
  20. Turk BT, Ates M, Sen BH. The effect of treatment of radicular dentin on colonization patterns of C. Albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106(3):457–462. DOI: 10.1016/j.tripleo.2008.05.012.
  21. Sen BH, Chugal NM, Liu H, et al. A new method for studying the adhesion of Candida albicans to dentin in the presence or absence of smear layer. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;96(2):201–206. DOI: 10.1016/s1079-2104(03)00165-3.
  22. Douglas LJ. Candida biofilms and their role in infection. Trends Microbiol 2003;11(1):30–36. DOI: 10.1016/s0966-842x(02) 00002-1.
  23. Vianna ME, Gomes BPFA, Berber VB, et al. In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97(1):79–84. DOI: 10.1016/s1079-2104(03)00360-3.
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