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VOLUME 21 , ISSUE 9 ( September, 2020 ) > List of Articles


Synergistic Effect of Arginine on Remineralization Potential of Fluoride Varnish and Nanohydroxyapatite on Artificial Caries Lesions: An In Vitro Study

Ravi K Konagala, Jyothi Mandava, Anupreeta Anwarullah, Lakshman V Uppalapati, Srujana Karumuri, Priyanka L Angadala

Keywords : Arginine, Energy-dispersive X-ray analysis, Fluoride varnish, Microhardness, Nanohydroxyapatite, Remineralization

Citation Information : Konagala RK, Mandava J, Anwarullah A, Uppalapati LV, Karumuri S, Angadala PL. Synergistic Effect of Arginine on Remineralization Potential of Fluoride Varnish and Nanohydroxyapatite on Artificial Caries Lesions: An In Vitro Study. J Contemp Dent Pract 2020; 21 (9):1048-1053.

DOI: 10.5005/jp-journals-10024-2915

License: CC BY-NC 4.0

Published Online: 22-01-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Aim and objective: To evaluate the synergistic effect of arginine on the remineralizing potential of fluoride varnish and nanohydroxyapatite. Materials and methods: A total of 100 teeth were taken; of them, 50 teeth were allotted for microhardness test and 50 teeth for scanning-electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) analysis. Fifty teeth used for hardness testing were sectioned to obtain 100 samples, and the baseline hardness values were measured. Samples were allotted into five groups (n = 20): group I: arginine; group II: fluoride varnish; group III: nanohydroxyapatite; group IV: arginine + fluoride varnish; group V: arginine + nanohydroxyapatite. Microhardness values were measured after 96 hours of demineralization and then again after application of remineralizing agents (pH cycling) for 10 days to check for gain in microhardness. The other 50 samples were subjected to SEM-EDX analysis for evaluating gain in the mineral content after demineralization and after application of the remineralizing agents. The collected data were subjected to statistical analysis using SPSS software version 22.0. Results: The maximum mean microhardness values were observed in group IV and group V. There was no statistical significance between them. Similarly, maximum mineral gain was seen in groups IV and V. A significant increase in fluoride gain was seen in group IV. Conclusion: Arginine has a synergistic effect on remineralization potential of fluoride varnish and nanohydroxyapatite. Clinical significance: The incorporation of arginine into fluoride varnishes and nanohydroxyapatite significantly increased their remineralization potential.

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