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VOLUME 21 , ISSUE 9 ( September, 2020 ) > List of Articles

ORIGINAL RESEARCH

Synergistic Effect of Arginine on Remineralization Potential of Fluoride Varnish and Nanohydroxyapatite on Artificial Caries Lesions: An In Vitro Study

Ravi K Konagala, Jyothi Mandava, Anupreeta Anwarullah, Lakshman V Uppalapati, Srujana Karumuri, Priyanka L Angadala

Keywords : Arginine, Energy-dispersive X-ray analysis, Fluoride varnish, Microhardness, Nanohydroxyapatite, Remineralization

Citation Information : Konagala RK, Mandava J, Anwarullah A, Uppalapati LV, Karumuri S, Angadala PL. Synergistic Effect of Arginine on Remineralization Potential of Fluoride Varnish and Nanohydroxyapatite on Artificial Caries Lesions: An In Vitro Study. J Contemp Dent Pract 2020; 21 (9):1048-1053.

DOI: 10.5005/jp-journals-10024-2915

License: CC BY-NC 4.0

Published Online: 21-10-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim and objective: To evaluate the synergistic effect of arginine on the remineralizing potential of fluoride varnish and nanohydroxyapatite. Materials and methods: A total of 100 teeth were taken; of them, 50 teeth were allotted for microhardness test and 50 teeth for scanning-electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) analysis. Fifty teeth used for hardness testing were sectioned to obtain 100 samples, and the baseline hardness values were measured. Samples were allotted into five groups (n = 20): group I: arginine; group II: fluoride varnish; group III: nanohydroxyapatite; group IV: arginine + fluoride varnish; group V: arginine + nanohydroxyapatite. Microhardness values were measured after 96 hours of demineralization and then again after application of remineralizing agents (pH cycling) for 10 days to check for gain in microhardness. The other 50 samples were subjected to SEM-EDX analysis for evaluating gain in the mineral content after demineralization and after application of the remineralizing agents. The collected data were subjected to statistical analysis using SPSS software version 22.0. Results: The maximum mean microhardness values were observed in group IV and group V. There was no statistical significance between them. Similarly, maximum mineral gain was seen in groups IV and V. A significant increase in fluoride gain was seen in group IV. Conclusion: Arginine has a synergistic effect on remineralization potential of fluoride varnish and nanohydroxyapatite. Clinical significance: The incorporation of arginine into fluoride varnishes and nanohydroxyapatite significantly increased their remineralization potential.


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  1. Fejerskov O. Changing paradigms in concepts on dental caries: consequences for oral health care. Caries Res 2004;38(3):182–191. DOI: 10.1159/000077753.
  2. Kidd E, Fejerskov O. What constitutes dental caries? Histopathology of carious enamel and dentin related to the action of cariogenic biofilms. J Dent Res 2004;83(1_suppl):35–38. DOI: 10.1177/154405910408301s07.
  3. GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the global burden of disease study 2016. Lancet 2017;390(10100):1211–1259. DOI: 10.1016/S0140-6736(17)32154-2.
  4. Christensen GJ. The advantages of minimally invasive dentistry. J Am Dent Assoc 2005;136(11):1563–1565. DOI: 10.14219/jada.archive.2005.0088.
  5. ten Cate JM. Remineralization of caries lesions extending into dentin. J Dent Res 2001;80(5):1407–1411. DOI: 10.1177/00220345010800050401.
  6. Ten Cate JM. Remineralization of deep enamel dentine caries lesions. Aust Dent J 2008;53(3):281–285. DOI: 10.1111/j.1834-7819.2008.00063.x.
  7. Featherstone JD. Fluoride, remineralization and root caries. Am J Dent 1994;7(5):271–274.
  8. Carvalho DM, Salazar M, Oliveira BH, et al. Fluoride varnishes and decrease in caries incidence in preschool children: a systematic review. Rev Bras Epidemiol 2010;13(1):139–149. DOI: 10.1590/s1415-790x2010000100013.
  9. Salehzadeh Esfahani K, Mazaheri R, Pishevar L. Effects of treatment with various remineralizing agents on the microhardness of demineralized enamel surface. J Dent Res Dent Clin Dent Prospects 2015;9(4):239–245. DOI: 10.15171/joddd.2015.043.
  10. Vandiver J, Dean D, Patel N, et al. Nanoscale variation in surface charge of synthetic hydroxyapatite detected by chemically and spatially specific high-resolution force spectroscopy. Biomaterials 2005;26(3):271–283. DOI: 10.1016/j.biomaterials.2004.02.053.
  11. Huang S, Gao S, Cheng L, et al. Remineralization potential of nano-hydroxyapatite on initial enamel lesions: an in vitro study. Caries Res 2011;45(5):460–468. DOI: 10.1159/000331207.
  12. Huang S, Gao S, Cheng L, et al. Combined effects of nano-hydroxyapatite and galla chinensis on remineralisation of initial enamel lesion in vitro. J Dent 2010;38(10):811–819. DOI: 10.1016/j.jdent.2010.06.013.
  13. Landis W, Jacquet R. Association of calcium and phosphate ions with collagen in the mineralization of veterbrate tissues. Calcif Tis Int 2013;93(4):329–337. DOI: 10.1007/s00223-013-9725-7.
  14. Silver FH, Landis WJ. Deposition of apatite in mineralizing vertebrate extracellular matrices: a model of possible nucleation sites on type I collagen. Connect Tissue Res 2011;52(3):242–254. DOI: 10.3109/03008207.2010.551567.
  15. Cheng X, Xu P, Zhou X, et al. Arginine promotes fluoride uptake into artificial carious lesions in vitro. Aust Dent J 2015;60(1):104–111. DOI: 10.1111/adj.12278.
  16. Wolff MS, Schenkel AB. The anticaries efficacy of a 1.5% arginine and fluoride toothpaste. Adv Dent Res 2018;29(1):93–97. DOI: 10.1177/0022034517735298.
  17. Ripa LW. Review of the anticaries effectiveness of professionally applied and self-applied topical fluoride gels. J Public Health Dent 1989;49(5 Spec No):297–309. DOI: 10.1111/j.1752-7325.1989.tb02088.x.
  18. ten Cate J, Duijsters P. Influence of fluoride in solution on tooth demineralization: I. chemical-data. Caries Res 1983;17(3):193–199. DOI: 10.1159/000260667.
  19. Stookey GK. The effect of saliva on dental caries. J Am Dent Assoc 2008;139(Suppl):11s–17s. DOI: 10.14219/jada.archive.2008.0347.
  20. Silverstone LM. Remineralization of human enamel in vitro. Proc R Soc Med 1972;65(10):906–908. DOI: 10.1177/003591577206501059.
  21. Grandjean P, Landrigan PJ. Neurobehavioural effects of developmental toxicity. Lancet Neurol 2014;13(3):330–338. DOI: 10.1016/S1474-4422(13)70278-3.
  22. Song J, Malathong V, Bertozzi CR. Mineralization of synthetic polymer scaffolds: a bottom-up approach for the development of artificial bone. J Am Chem Soc 2005;127(10):3366–3372. DOI: 10.1021/ja043776z.
  23. George A, Veis A. Phosphorylated proteins and control over apatite nucleation, crystal growth, and inhibition. Chem Rev 2008;108(11):4670–4693. DOI: 10.1021/cr0782729.
  24. Wang H, Xiao Z, Yang J, et al. Oriented and ordered biomimetic remineralization of the surface of demineralized dental enamel using HAP@ACP nanoparticles guided by glycine. Sci Rep 2017;7(1):40701. DOI: 10.1038/srep40701.
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