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VOLUME 22 , ISSUE 11 ( November, 2021 ) > List of Articles

ORIGINAL RESEARCH

Effect of Denture Adhesives on Adhesion of Candida albicans to Denture Base Materials: An In Vitro Study

Mahmoud Darwish, Mohammad Z Nassani, Khaled R Al-Hallak, Omar Kujan

Keywords : Candida adhesion, Dentures adhesives, Denture bases

Citation Information : Darwish M, Nassani MZ, Al-Hallak KR, Kujan O. Effect of Denture Adhesives on Adhesion of Candida albicans to Denture Base Materials: An In Vitro Study. J Contemp Dent Pract 2021; 22 (11):1257-1261.

DOI: 10.5005/jp-journals-10024-3209

License: CC BY-NC 4.0

Published Online: 24-02-2022

Copyright Statement:  Copyright © 2021; The Author(s).


Abstract

Aim and objective: This study aimed to investigate the adhesion of Candida albicans (CA) to the surface of the denture base using two different types of resins after exposure to denture adhesives (DAs). Materials and methods: Twenty-five samples of polymethyl methacrylate (PMMA) and 25 samples of urethane dimethacrylate (UDMA) (10 mm × 10 mm × 2 mm) were prepared and allocated to 10 groups (five samples in each group). For each type of denture base materials, four test groups were immersed for 30 days in four commercially available DAs in addition to water as control group. Specimens were then in vitro evaluated for CA adhesion using light microscopy under × 1,000 magnification. Results: The results showed that the mean number of CA per specimen in the adhesive-exposed groups was higher than that in the control group for both UDMA and PMMA resins. However, this difference was not statistically significant. No association between the type of denture base material and Candida count was identified following immersion in different types of DA and distilled water (p >0.05). Candida count was not associated with the type of DA when UDMA specimens were compared (p >0.05). PMMA resin specimens that were immersed in Corega cream showed significantly higher counts of Candida compared to control specimens that were immersed in distilled water (p <0.05). Conclusion: The study found no association between the type of denture base materials and CA adhesion. No clear impact was identified for the type of DA on CA adhesion to acrylic denture bases. Clinical significance: Using DAs would likely not increase the incidence of denture stomatitis in completely edentulous patients.


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  1. Oliveira MC, Oliveira VM, Vieira AC, et al. In vivo assessment of the effect of an adhesive for complete dentures on colonization of Candida species. Gerodontology 2010;27(4):303–307. DOI: 10.1111/j.1741-2358.2009.00345.x.
  2. Richardson M, Lass-Florl C. Changing epidemiology of systemic fungal infections. Clin Microbiol Infect 2008;14(Suppl 4):5–24. DOI: 10.1111/j.1469-0691.2008.01978.x.
  3. Morschhauser J. Regulation of multidrug resistance in pathogenic fungi. Fungal Genet Biol 2010;47(2):94–106. DOI: 10.1016/j.fgb.2009.08.002.
  4. Al-Dwairi ZN, Al-Quran FA, Al-Omari OY. The effect of antifungal agents on surface properties of poly (methyl methacrylate) and its relation to adherence of Candida albicans. J Prosthodont Res 2012;56(4):272–280. DOI: 10.1016/j.jpor.2012.02.006.
  5. Cannon RD, Chaffin WL. Oral colonization by Candida albicans. Crit Rev Oral Biol Med 1999;10(3):359–383. DOI: 10.1177/10454411990100030701.
  6. Chandra J, Mukherjee PK, Leidich SD, et al. Antifungal resistance of candidal biofilms formed on denture acrylic in vitro. J Dent Res 2001;80(3):903–908. DOI: 10.1177/00220345010800031101.
  7. Ramage G, Tomsett K, Wickes BL, et al. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;98(1):53–59. DOI: 10.1016/j.tripleo.2003.04.002.
  8. Branting C, Sund ML, Linder LE. The influence of Streptococcus mutans on adhesion of Candida albicans to acrylic surfaces in vitro. Arch Oral Biol 1989;34(5):347–353. DOI: 10.1016/0003-9969(89)90108-8.
  9. Park SA, Blissett R, Susarla SM, et al. Candida albicans adherence to surface-modified denture resin surfaces. J Prosthodont 2008;17(5):365–369. DOI: 10.1111/j.1532-849X.2007.00292.x.
  10. Bridgett MJ, Davies MC, Denyer SP, et al. In vitro assessment of bacterial adhesion to Hydromer-coated cerebrospinal fluid shunts. Biomaterials 1993;14(3):184–188. DOI: 10.1016/0142-9612(93)90021-s.
  11. Verheyen CC, Dhert WJ, de Blieck-Hogervorst JM, et al. Adherence to a metal, polymer and composite by Staphylococcus aureus and Staphylococcus epidermidis. Biomaterials 1993;14(5):383–391. DOI: 10.1016/0142-9612(93)90059-b.
  12. Minagi S, Miyake Y, Inagaki K, et al. Hydrophobic interaction in Candida albicans and Candida tropicalis adherence to various denture base resin materials. Infect Immun 1985;47(1):11–14. DOI: 10.1128/iai.47.1.11-14.1985.
  13. Koch C1, Bürgers R, Hahnel S. Candida albicans adherence and proliferation on the surface of denture base materials. Gerodontology 2013;30:309–313. DOI: 10.1111/ger.12056.
  14. Kalra P, Nadiger R, Shah FK. An investigation into the effect of denture adhesives on incisal bite force of complete denture wearers using pressure transducers- a clinical study. J Adv Prosthodont 2012;4(2):97–102. DOI: 10.4047/jap.2012.4.2.97.
  15. Zhao K, Cheng XR, Chao YL, et al. Laboratory evaluation of a new denture adhesive. Dent Mater 2004;20(5):419–424. DOI: 10.1016/j.dental.2002.12.001.
  16. Adisman IK. The use of denture adhesives as an aid to denture treatment. J Prosthet Dent 1989;62(6):711–715. DOI: 10.1016/0022-3913(89)90598-2.
  17. Stafford GD, Russell C. Efficiency of denture adhesives and their possible influence on oral microorganisms. J Dent Res 1971;50(4): 832–836. DOI: 10.1177/00220345710500040701.
  18. Makihira S, Nikawa H, Satonobu SV, et al. Growth of Candida species on commercial denture adhesives in vitro. Int J Prosthodont 2001;14(1):48–52. PMID: 11842904.
  19. Kim E, Driscoll CF, Minah GE. The effect of a denture adhesive on the colonization of Candida species in vivo. J Prosthodont 2003;12(3): 187–191. DOI: 0.1016/S1059-941X(03)00050-0.
  20. Sampaio-Maia B, Figueiral MH, Sousa-Rodrigues P, et al. The effect of denture adhesives on Candida albicans growth in vitro. Gerodontology 2012;29(2):348–356. DOI: 10.1111/j.1741-2358. 2011.00478.x.
  21. Arendorf TM, Walker DM. Denture stomatitis: a review. J Oral Rehabil 1987;14(3):217–227. DOI: 10.1111/j.1365-2842.1987.tb00713.x.
  22. Love WB, Biswas S. Denture adhesives--pH and buffering capacity. J Prosthet Dent 1991;66(3):356–360. DOI: 10.1016/0022-3913(91)90263-v.
  23. Raut JS, Ravikumar BS, Nitin MC, et al. Phenylpropanoids of plant origin as inhibitors of biofilm formation by Candida albicans. J Microbiol Biotechnol 2014;24(9):1216–1225. DOI: 10.4014/jmb.1402.02056.
  24. Darwish M, Nassani MZ. Evaluation of the effect of denture adhesives on surface roughness of two chemically different denture base resins. Eur J Dent 2016;10(3):321–326. DOI: 10.4103/1305-7456.184155.
  25. Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin and silicone of different surface topography. J Prosthet Dent 1997;77(5):535–539. DOI: 10.1016/s0022-3913(97)70148-3.
  26. Radford DR, Sweet SP, Challacombe SJ, et al. Adhesion of Candida albicans to denture-base materials with different surface finishes. J Dent 1998;26(7):577–583. DOI: 10.1016/s0300-5712(97)00034-1.
  27. Taylor R, Maryan C, Verran J. Retention of oral microorganisms on cobalt-chromium alloy and dental acrylic resin with different surface finishes. J Prosthet Dent 1998;80(5):592–597. DOI: 10.1016/s0022-3913(98)70037-x.
  28. Radford DR, Challacombe SJ, Walter JD. Denture plaque and adherence of Candida albicans to denture-base materials in vivo and in vitro. Crit Rev Oral Biol Med 1999;10(1):99–116. DOI: 10.1177/10454411990100010501.
  29. Whitehead KA, Verran J. The effect of surface topography on the retention of microorganisms. Food Bioprod Process 2006;84(4): 253–259. DOI: 10.1205/fbp06035.
  30. Al-Bakri IA, Harty D, Al-Omari WM, et al. Surface characteristics and microbial adherence ability of modified polymethylmethacrylate by fluoridated glass fillers. Aust Dent J 2014;59(4):482–489. DOI: 10.1111/adj.12218.
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