Single Nucleotide Polymorphisms of BMP2 Gene Association with Skeletal Class I Crowding: A PCR Study
Kalpesh Sharma, Vijay N Yannawar, Ganesh R Kotalwar, Pallavi Madanshetty, Ishita Grover, Prashant S Lagali
BMP2 gene, DNA sequencing, Genetic markers, Single nucleotide polymorphism, Skeletal class I
Citation Information :
Sharma K, Yannawar VN, Kotalwar GR, Madanshetty P, Grover I, Lagali PS. Single Nucleotide Polymorphisms of BMP2 Gene Association with Skeletal Class I Crowding: A PCR Study. J Contemp Dent Pract 2021; 22 (3):248-252.
Aim and objective: This study is conducted to find the association of BMP2 (bone morphogenic protein 2) gene variant rs1005464 and rs15705 with skeletal class I crowding cases.
Materials and methods: Blood samples from 60 subjects who visited the Department of Orthodontics and Dentofacial Orthopaedics, D.A.P.M.R.V. Dental College, Bengaluru, were taken after written informed consent. These were divided into two groups: group A with 30 subjects having skeletal class I bases with crowding and group B with 30 subjects having skeletal class I bases without visible crowding or spacing (±2 mm). Around 2 mL of venous blood sample was procured from cases and controls after careful examination. All the samples were then subjected to polymerase chain reaction followed by DNA sequencing and capillary electrophoresis. BMP2 rs1005464 and rs15705 gene variants were assessed and Z-Test was used for statistical analysis.
Result: GG (p = 0.001) and CC (p = 0.0024) genotype of BMP2 gene variant rs1005464 and rs15705, respectively, are significantly associated with skeletal class I crowding cases.
Conclusion: This study concludes that BMP2 variants rs1005464 and rs15705 can be used as genetic markers for skeletal class I bases having crowding.
Clinical significance: Predisposing genetic markers BMP2 can be identified prior and this would help in predicting the probability of potential crowding in the future and this would help in early prevention and intervention of crowding.
Graber TM, Vanarsdall RL. Orthodontics: Current Principles and Techniques. St Louis: Mosby-Year Book; 1994.
Ting TY, Wong RWK, Rabie ABM. Analysis of genetic polymorphisms in skeletal class I crowding. Am J Orthod Dentofacial Orthop 2011;140(1):e9–e15. DOI: 10.1016/j.ajodo.2010.12.015.
Baker CR. Similarity of malocclusion in families. Int J Orthod 1924;10(8):459–462. DOI: 10.1016/S0099-6963(24)90032-9.
Korkhaus G. Anthropologic and odontologic studies of twins. Int J Orthodont 1930;16(6):640–647. DOI: 10.1016/S0099-6963(30)90323-7.
Corruccini RS, Potter RH. Genetic analysis of occlusal variation in twins. Am J Orthod 1980;78(2):140–154. DOI: 10.1016/0002-9416(80)90056-1.
Mavropoulos A, Kiliaridis S. Orthodontic literature: an overview of the last 2 decades. Am J Orthod Dentofacial Orthop 2003;124(1):30–40. DOI: 10.1016/s0889-5406(03)00199-9.
Human genome. International Human Genome Consortium: Initial sequencing and analysis of the human genome. Nature 2001;409:860–921.
Baik CY, Ververidou M. A new approach of assessing sagittal discrepancies: the beta angle. Am J Orthod 2004;126(1):100–105. DOI: 10.1016/S0889540604002483.
Vaahtokari A, Aberg T, Jernvall J. The enamel knot as a signalling center in the developing mouse tooth. Mech Dev 1996;54(1):39–43. DOI: 10.1016/0925-4773(95)00459-9.
Garib DG. Associated dental anomalies: the orthodontist decoding the genetics which regulates the dental development disturbances. Dental Press J Orthod 2010;15(2):138–157.
Wei F, Vergnes J, Vergnes JN, et al. Asynchronous dentofacial development and dental crowding: a cross-sectional study in a contemporary sample of children. J Physiol Anthropol 2013;32(1):22. DOI: 10.1186/1880-6805-32-22.
Cobourne MT. The genetic control of early odontogenesis. Br J Orthod 1999;26(1):21–28. DOI: 10.1093/ortho/26.1.21.
Feng J, Yang G, Yuan G. Abnormalities in the enamel in BMP2-deficient mice. Cells Tissues Organs 2011;194(2–4):216–221. DOI: 10.1159/000324644.
Matsumoto T, Yamada A, Aizawa R, et al. BMP-2 induced expression of Alx3 that is a positive regulator of osteoblast differentiation. PLoS One 2013;8(6):e68774. DOI: 10.1371/journal.pone.0068774.
Miyoshi K, Nagata H, Horiguchi T, et al. BMP2-induced gene profiling in dental epithelial cell line. J Med Invest 2008;55(3–4):216–226. DOI: 10.2152/jmi.55.216.
Dolnik D. DNA sequencing by capillary electrophoresis (review). J Biochem Biophys Methods 1999;41(2–3):103–119. DOI: 10.1016/S0165-022X(99)00041-X.