The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 22 , ISSUE 3 ( March, 2021 ) > List of Articles


Does Cavity Disinfectant Affect Sealing Ability of Universal Self-etch Adhesive?

Prasanti Kumari Pradhan, Kanhu C Sahoo, Khushboo Ghosh, S Lata, Gaurav Patri, Subhasmita Bhol

Keywords : Caries, Cavity disinfectant, Chitosan, Chlorhexidine, Octenidine dihydrochloride

Citation Information : Pradhan PK, Sahoo KC, Ghosh K, Lata S, Patri G, Bhol S. Does Cavity Disinfectant Affect Sealing Ability of Universal Self-etch Adhesive?. J Contemp Dent Pract 2021; 22 (3):273-278.

DOI: 10.5005/jp-journals-10024-3052

License: CC BY-NC 4.0

Published Online: 27-01-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Aim: The purpose of this study was to evaluate 2% chlorhexidine disinfectant (CHX), chitosan, and octenidine dihydrochloride (as cavity disinfectants) on microleakage in cavities restored with universal self-etch adhesive. Materials and methods: Eighty extracted human permanent premolars were selected. Class V cavities were prepared on the facial surface of each tooth. The teeth were then divided into four groups of 20 teeth each. For the control group after cavity preparation, no disinfectant was applied. The other 3 groups were treated with 0.1% chitosan, 2% CHX, and 0.1% octenidine dihydrochloride (OCT). All the groups were restored with universal adhesive followed by composite resin. The teeth were then immersed in 1% methylene blue dye and were sectioned buccolingually. Microleakage was checked under a stereomicroscope on both occlusal and gingival margins. Result: Among all the groups chitosan-treated cavities showed the least microleakage. Chlorhexidine treated cavities showed less leakage as compared to control, OCT group at both the margins. Conclusion: Chitosan as a cavity disinfectant improves the sealing ability of the self-etch adhesive. Furthermore, in vivo studies need to be conducted to examine the interaction and long-term effect of chitosan with the other self-etch adhesive systems. Clinical significance: Chitosan a natural polysaccharide can be used as a cavity disinfectant as it improves the sealing ability of self-etch adhesive.

  1. Gürgan S, Bolay S, Kiremitçi A. Effect of disinfectant application methods on the bond strength of composite to dentin. J Oral Rehabil 1999;26(10):836–840. DOI: DOI: 10.1046/j.1365-2842.1999.00458.x.
  2. Sharma V, Rampal P, Kumar S. Shear bond strength of composite resin to dentin after application of cavity disinfectants—SEM study. Contemp Clin Dent 2011;2(3):155–159. DOI: 10.4103/0976-237X.86438.
  3. Meiers JC, Kresin JC. Cavity disinfectants and dentin bonding. Oper Dent 1996;21(4):153–159.
  4. Singla M, Aggarwal V, Kumar N. Effect of chlorhexidine cavity disinfection on microleakage in cavities restored with composite using a self-etching single bottle adhesive. J Conserv Dent 2011;14(4):374–377. DOI: 10.4103/0972-0707.87201.
  5. Hebling J, Pashley DH, Tjäderhane L, et al. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 2005;84(8):741–746. DOI: 10.1177/154405910508400811.
  6. Goy RC, de Britto D, Assis OBG. A review of the antimicrobial activity of chitosan. Polímeros 2009;19(3):241–247. DOI: 10.1590/S0104-14282009000300013.
  7. Bae K, Jun EJ, Lee SM, et al. Effect of water-soluble reduced chitosan on Streptococcus mutans, plaque regrowth and biofilm vitality. Clin Oral Invest 2006;10(2):102–107. DOI: 10.1007/s00784-006-0038-3.
  8. Aliasghari A, Rabbani Khorasgani M, Vaezifar S, et al. Evaluation of antibacterial efficiency of chitosan and chitosan nanoparticles on cariogenic streptococci: an in vitro study. Iran J Microbiol 2016;8(2):93–100.
  9. Ururahy MS, Curylofo-Zotti FA, Galo R, et al. Wettability and surface morphology of eroded dentin treated with chitosan. Arch Oral Biol 2017;75:68–73. DOI: 10.1016/j.archoralbio.2016.11.017.
  10. Sedlock DM, Bailey DM. Microbicidal activity of octenidine hydrochloride, a new alkanediylbis[pyridine] germicidal agent. Antimicrob Agents Chemother 1985;28(6):786–790. DOI: 10.1128/aac.28.6.786.
  11. Munro GA, Hilton TJ, Hermesch CB. In vitro microleakage of etched and rebonded class V composite resin restorations. Oper Dent 1996;21(5):203–208.
  12. Siso HS, Kustarci A, Göktolga EG. Microleakage in resin composite restorations after antimicrobial pre-treatments: effect of KTP laser, chlorhexidine gluconate and Clearfil Protect Bond. Oper Dent 2009;34(3):321–327. DOI: 10.2341/08-96.
  13. Ramasetty PA, Tripathi AP, Sugandhan S, et al. Nanotechnology in Dentin Disinfection: Can We Preserve the Bond? Int J Clin Pediatr Dent 2018;11(6):468–473. DOI: 10.5005/jp-journals-10005-1559.
  14. Gupta A, Tavane P, Gupta PK, et al. Evaluation of microleakage with total etch, self etch and universal adhesive systems in class V restorations: an in vitro study. J Clin Diagn Res 2017;11(4):ZC53–ZC56. DOI: 10.7860/JCDR/2017/24907.9680.
  15. Van Meerbeek B, De Munck J, Yoshida Y, et al. Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent 2003;28(3):215–235.
  16. Fawzy AS, Nitisusanta LI, Iqbal K, et al. Chitosan/riboflavin-modified demineralized dentin as a potential substrate for bonding. J Mech Behav Biomed Mater 2013;17:278–289. DOI: 10.1016/j.jmbbm.2012.09.008.
  17. Silva PV, Guedes DF, Pécora JD, et al. Time-dependent effects of chitosan on dentin structures. Braz Dent J 2012;23(4):357–361. DOI: 10.1590/s0103-64402012000400008.
  18. Cardoso MV, de Almeida Neves A, Mine A, et al. Current aspects on bonding effectiveness and stability in adhesive dentistry. Aust Dent J 2011;56 Suppl 1:31–44. DOI: 10.1111/j.1834-7819.2011.01294.x.
  19. Owens BM, Johnson WW. Effect of single step adhesives on the marginal permeability of Class V resin composites. Oper Dent 2007;32(1):67–72. DOI: 10.2341/06-17.
  20. Stanislawczuk R, Amaral RC, Zander-Grande C, et al. Chlorhexidine-containing acid conditioner preserves the longevity of resin-dentin bonds. Oper Dent 2009;34(4):481–490. DOI: 10.2341/08-016-L.
  21. Pilo R, Cardash HS, Oz-Ari B, et al. Effect of preliminary treatment of the dentin surface on the shear bond strength of resin composite to dentin. Oper Dent 2001;26(6):569–575.
  22. Carrilho MR, Carvalho RM, de Goes MF, et al. Chlorhexidine preserves dentin bond in vitro. J Dent Res 2007;86(1):90–94. DOI: 10.1177/154405910708600115.
  23. Biermann CD, Kribs A, Roth B, et al. Use and cutaneous side effects of skin antiseptics in extremely low birth weight infants—a retrospective survey of the German NICUs. Klin Padiatr 2016;228(4):208–212. DOI: 10.1055/s-0042-104122.
  24. Cherian B, Gehlot PM, Manjunath MK. Comparison of the antimicrobial efficacy of octenidine dihydrochloride and chlorhexidine with and without passive ultrasonic irrigation—an invitro study. J Clin Diagn Res 2016;10(6):ZC71–ZC77. DOI: 10.7860/JCDR/2016/17911.8021.
  25. Chum JD, Lim DJZ, Sheriff SO, et al. In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system. Restor Dent Endod 2019;44(1):e8. DOI: 10.5395/rde.2019.44.e8.
  26. Kaushik M, Sheoran K, Reddy P, et al. Comparison of the effect of three different irrigants on the contact angle of an epoxy resin sealer with intraradicular dentin. Saudi Endod J 2015;5(3):166–170. DOI: 10.4103/1658-5984.163627.
  27. Rosales-Leal JI. Microleakage of class V composite restorations placed with etch-and-rinse and self-etching adhesives before and after thermocycling. J Adhes Dent 2007;9 Suppl 2:255–259.
  28. Chandra PVR, Harikumar V, Ramkiran D, et al. Microleakage of class V resin composites using various self-etching adhesives: an in vitro study. J Contemp Dent Pract 2013;14(1):51–55. DOI: 10.5005/jp-journals-10024-1269.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.