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VOLUME 22 , ISSUE 5 ( May, 2021 ) > List of Articles

ORIGINAL RESEARCH

Influence of Bleaching Gel Peroxide Concentration on Color and Penetration through the Tooth Structure

Carlos RG Torres, Rayssa F Zanatta, Monique MM Godoy, Alessandra B Borges

Keywords : Bleaching, Color, Concentration, Peroxide

Citation Information : Torres CR, Zanatta RF, Godoy MM, Borges AB. Influence of Bleaching Gel Peroxide Concentration on Color and Penetration through the Tooth Structure. J Contemp Dent Pract 2021; 22 (5):479-483.

DOI: 10.5005/jp-journals-10024-3023

License: CC BY-NC 4.0

Published Online: 09-07-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim and objective: The purpose of this study was to assess the effect of hydrogen peroxide concentration on the bleaching efficacy and penetration through the tooth structure. Materials and methods: One hundred enamel/dentin specimens with cylindrical shape were obtained from bovine incisors. The surfaces were polished and the size standardized. They were divided into five groups (n = 20), following the concentration of hydrogen peroxide in the bleaching gels: 20, 25, 30, 35, and 40% (w/w). The specimens were placed over artificial pulpal chambers containing acetate buffer solution and bleached for 30 minutes (three applications of 10 minutes each). Aliquots of the acetate solution were collected, and the peroxide concentration was measured by an analytic spectrophotometer. The color of the samples was analyzed using a colorimetric spectrophotometer at the baseline and 7 days after the bleaching procedure. The color difference was calculated using the ΔEab formula. The data were analyzed by one-way ANOVA and Tukey\'s test (p <0.05). Results: The peroxide concentrations of 20–30% showed smaller bleaching effect than the higher concentrations (p = 0.001). The peroxide penetration was significantly higher (p = 0.001) for the more concentrated gels (35 and 40%). Conclusion: The higher peroxide concentrations enhance the bleaching efficacy, but also increased the peroxide penetration through the tooth structure. Clinical significance: In-office bleaching gels with higher concentrations of hydrogen peroxide (35 and 40%) present superior whitening efficacy. Nevertheless, they might also intensify the negative biological effects on the pulpal tissue, since they exhibit increased penetration potential.


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  1. Caneppele TM, Borges AB, Torres CRG. Effects of dental bleaching on the color, translucency and fluorescence properties of enamel and dentin. Eur J Esthet Dent 2013;8(2):200–212. PMID: 23712341.
  2. Torres CRG, Wiegand A, Sener B, et al. Influence of chemical activation of a 35% hydrogen peroxide bleaching gel on its penetration and efficacy-in vitro study. J Dent 2010;38(10):838–846. DOI: 10.1016/j.jdent.2010.07.002.
  3. Torres CRG, Guimarães CA, Ribeiro ZEA, et al. Influence of different types and concentrations of chemical catalysts on dental bleaching efficiency. J Contemp Dent Pract 2015;16(11):893–902. DOI: 10.5005/jp-journals-10024-1778.
  4. Borges AB, Zanatta RF, Barros ACSM, et al. Effect of hydrogen peroxide concentration on enamel color and microhardness. Oper Dent 2015;40(1):96–101. DOI: 10.2341/13-371-L.
  5. Reis A, Dalanhol AP, Cunha TS, et al. Assessment of tooth sensitivity using a desensitizer before light-activated bleaching. Oper Dent 2011;36(1):12–17. DOI: 10.2341/10-148-CR.
  6. Mena-Serrano AP, Parreiras SO, Nascimento EMS, et al. Effects of the concentration and composition of in-office bleaching gels on hydrogen peroxide penetration into the pulp chamber. Oper Dent 2015;40(2):E76-E82. DOI: 10.2341/13-352-L.
  7. Torres CRG, Crastechini E, Feitosa FA, et al. Influence of pH on the effectiveness of hydrogen peroxide whitening. Oper Dent 2014;39(6):E261–E268. DOI: 10.2341/13-214-L.
  8. Kwon SR, Pallavi FNU, Shi Y, et al. Effect of bleaching gel viscosity on tooth whitening efficacy and pulp chamber penetration: an in vitro study. Oper Dent 2018;43(3):326–334. DOI: 10.2341/17-099-L.
  9. do Carmo Públio J, Zeczkowski M, Burga-Sánchez J, et al. Influence of different thickeners in at-home tooth bleaching: a randomized clinical trial study. Clin Oral Investig 2019;23(5):2187–2198. DOI: 10.1007/s00784-018-2613-9.
  10. Ito Y, Otsuki M, Tagami J. Effect of pH conditioners on tooth bleaching. Clin Exp Dent Res 2019;5(3):212–218. DOI: 10.1002/cre2.172.
  11. Caneppele TMF, Torres CRG. Influence of surfactants on the effectiveness of bleaching gels. Clin Oral Investig 2011;15(1):57–64. DOI: 10.1007/s00784-009-0358-1.
  12. Magalhães JG, Marimoto ARK, Torres CRG, et al. Microhardness change of enamel due to bleaching with in-office bleaching gels of different acidity. Acta Odontol Scand 2012;70(2):122–126. DOI: 10.3109/00016357.2011.600704.
  13. Borges AB, Guimaraes CA, Bresciani E, et al. Effect of incorporation of remineralizing agents into bleaching gels on the microhardness of bovine enamel in situ. J Contemp Dent Pract 2014;15(2):195–201. DOI: 10.5005/jp-journals-10024-1514.
  14. Jurema ALB, de Souza MY, Torres CRG, et al. Effect of pH on whitening efficacy of 35% hydrogen peroxide and enamel microhardness. J Esthet Restor Dent 2018;30(2):E39–E44. DOI: 10.1111/jerd.12367.
  15. Borges AB, Batista GR, Arantes PT, et al. Influence of simulated pulpal pressure on efficacy of bleaching gels. J Contemp Dent Pract 2014;15(4):407–412. DOI: 10.5005/jp-journals-10024-1553.
  16. Torres CRG, Zanatta RF, Silva TJ, et al. Effect of calcium and fluoride addition to hydrogen peroxide bleaching gel on tooth diffusion, color, and microhardness. Oper Dent 2019;44(4):424–432. DOI: 10.2341/18-113-L.
  17. Lima LF, Alencar AHG, Decurcio DA, et al. Effect of dental bleaching on pulp oxygen saturation in maxillary central incisors - a randomized clinical trial. J Appl Oral Sci 2019;27(7):e20180442. DOI: 10.1590/1678-7757-2018-0442.
  18. Kina JF, Huck C, Riehl H, et al. Response of human pulps after professionally applied vital tooth bleaching. Int Endod J 2010;43(7):572–580. DOI: 10.1111/j.1365-2591.2010.01713.x.
  19. Vaz MM, Lopes LG, Cardoso PC, et al. Inflammatory response of human dental pulp to at-home and in-office tooth bleaching. J Appl Oral Sci 2016;24(5):509–517. DOI: 10.1590/1678-775720160137.
  20. Torres CRG, Souza CS, Borges AB, et al. Influence of concentration and activation on hydrogen peroxide diffusion through dental tissues in vitro. Sci World J 2013:193241. DOI: 10.1155/2013/193241.
  21. Marson FC, Gonçalves RS, Silva CO, et al. Penetration of hydrogen peroxide and degradation rate of different bleaching products. Oper Dent 2015;40(1):72–79. DOI: 10.2341/13-270-L.
  22. Bauminger BB. Micro method for manual analysis of true glucose in plasma without deproteinization. J Clin Pathol 1974;27(12):1015–1017. DOI: 10.1136/jcp.27.12.1015.
  23. Hannig C, Zech R, Henze E, et al. Determination of peroxides in saliva-kinetics of peroxide release into saliva during home-bleaching with whitestrips and vivastyle. Arch Oral Biol 2003;48(8):559–566. DOI: 10.1016/s0003-9969(03)00102-x.
  24. Tay LY, Kose C, Loguercio AD, et al. Assessing the effect of a desensitizing agent used before in-office tooth bleaching. J Am Dent Assoc 2009;140(10):1245–1251. DOI: 10.14219/jada.archive.2009.0047.
  25. Kawamoto K, Tsujimoto Y. Effects of the hydroxyl radical and hydrogen peroxide on tooth bleaching. J Endod 2004;30(1):45–50. DOI: 10.1097/00004770-200401000-00010.
  26. Soares DG, Ribeiro APD, da Silveira Vargas F, et al. Efficacy and cytotoxicity of a bleaching gel after short application times on dental enamel. Clin Oral Investig 2013;17(8):1901–1909. DOI: 10.1007/s00784-012-0883-1.
  27. Soares DG, Basso FG, Pontes ECV, et al. Effective tooth-bleaching protocols capable of reducing H2O2 diffusion through enamel and dentine. J Dent 2014;42(3):351–358. DOI: 10.1016/j.jdent.2013.09.001.
  28. Soares DG, Basso FG, Hebling J, et al. Concentrations of and application protocols for hydrogen peroxide bleaching gels: effects on pulp cell viability and whitening efficacy. J Dent 2014;42(2):185–198. DOI: 10.1016/j.jdent.2013.10.021.
  29. Sulieman M, Addy M, MacDonald E, et al. The effect of hydrogen peroxide concentration on the outcome of tooth whitening: an in vitro study. J Dent 2004;32(4):295–299. DOI: 10.1016/j.jdent.2004.01.003.
  30. Dietschi D, Rossier S, Krejci I. In vitro colorimetric evaluation of the efficacy of various bleaching methods and products. Quintessence Int 2006;37(7):515–526. PMID: 16841599.
  31. Almeida LCAG, Soares DG, Gallinari MO, et al. Color alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocols. Clin Oral Investig 2015;19(3):673–680. DOI: 10.1007/s00784-014-1285-3.
  32. Barbosa JG, Benetti F, de Oliveira Gallinari M, et al. Bleaching gel mixed with MI Paste Plus reduces penetration of H2O2 and damage to pulp tissue and maintains bleaching effectiveness. Clin Oral Investig 2020;24(3):1299–1309. DOI: 10.1007/s00784-019-03009-5.
  33. Reis A, Kossatz S, Martins GC, et al. Efficacy of and effect on tooth sensitivity of in-office bleaching gel concentrations: a randomized clinical trial. Oper Dent 2013;38(4):386–393. DOI: 10.2341/12-140-C.
  34. Gonçalves MLL, da Silva Tavares AC, da Mota ACC, et al. In-office tooth bleaching for adolescents using hydrogen peroxide-based gels. Braz Dent J 2017;28(6):720–725. DOI: 10.1590/0103-6440201701516.
  35. Rezende M, Loguercio AD, Kossatz S, et al. Predictive factors on the efficacy and risk/intensity of tooth sensitivity of dental bleaching: a multi regression and logistic analysis. J Dent 2016;45:1–6. DOI: 10.1016/j.jdent.2015.11.003.
  36. Alomari Q, El Daraa E. A randomized clinical trial of in-office dental bleaching with or without light activation. J Contemp Dent Pract 2010;11(1):E017–E024. DOI: 10.5005/jcdp-11-1-17.
  37. Coldebella CR, Ribeiro APD, Sacono NT, et al. Indirect cytotoxicity of a 35% hydrogen peroxide bleaching gel on cultured odontoblast-like cells. Braz Dent J 2009;20(4):267–274. DOI: 10.1590/s0103-64402009000400001.
  38. Markowitz K. Pretty painful: why does tooth bleaching hurt? Med Hypotheses 2010;74(5):835–840. DOI: 10.1016/j.mehy.2009.11.044.
  39. Cintra LTA, Benetti F, Ferreira LL, et al. Penetration capacity, color alteration and biological response of two in-office bleaching protocols. Braz Dent J 2016;27(2):169–175. DOI: 10.1590/0103-6440201600329.
  40. Costa CAS, Riehl H, Kina JF, et al. Human pulp responses to in-office tooth bleaching. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109(4):e59–e64. DOI: 10.1016/j.tripleo.2009.12.002.
  41. Bowles WH, Ugwuneri Z. Pulp chamber penetration by hydrogen peroxide following vital bleaching procedures. J Endod 1987;13(8):375–377. DOI: 10.1016/S0099-2399(87)80196-6.
  42. Benetti AR, Valera MC, Mancini MNG, et al. In vitro penetration of bleaching agents into the pulp chamber. Int Endod J 2004;37(2):120–124. DOI: 10.1111/j.0143-2885.2004.00761.x.
  43. Hanks CT, Fat JC, Wataha JC, et al. Cytotoxicity and dentin permeability of carbamide peroxide and hydrogen peroxide vital bleaching materials, in vitro. J Dent Res 1993;72(5):931–938. DOI: 10.1177/00220345930720051501.
  44. Benetti F, Gomes-Filho JE, Ferreira LL, et al. Concentration-dependent effect of bleaching agents on the immunolabelling of interleukin-6, interleukin-17 and CD5-positive cells in the dental pulp. Int Endod J 2018;51(7):789–799. DOI: 10.1111/iej.12891.
  45. Bowles WH, Thompson LR. Vital bleaching: the effects of heat and hydrogen peroxide on pulpal enzymes. J Endod 1986;12(3):108–112. DOI: 10.1016/S0099-2399(86)80300-4.
  46. Cintra LTA, Benetti F, Ferreira LL, et al. Evaluation of an experimental rat model for comparative studies of bleaching agents. J Appl Oral Sci 2016;24(1):95–104. DOI: 10.1590/1678-775720150393.
  47. Berger SB, Tabchoury CPM, Ambrosano GMB, et al. Hydrogen peroxide penetration into the pulp chamber and dental permeability after bleaching. Gen Dent 2013;61(3):e21–e25.
  48. Moncada G, Sepúlveda D, Elphick K, et al. Effects of light activation, agent concentration, and tooth thickness on dental sensitivity after bleaching. Oper Dent 2013;38(5):467–476. DOI: 10.2341/12-335-C.
  49. Ubaldini ALM, Baesso ML, Medina Neto A, et al. Hydrogen peroxide diffusion dynamics in dental tissues. J Dent Res 2013;92(7):661–665. DOI: 10.1177/0022034513488893.
  50. Briso ALF, Lima APB, Gonçalves RS, et al. Transenamel and transdentinal penetration of hydrogen peroxide applied to cracked or microabrasioned enamel. Oper Dent 2014;39(2):166–173. DOI: 10.2341/13-014-L.
  51. Kossatz S, Martins G, Loguercio AD, et al. Tooth sensitivity and bleaching effectiveness associated with use of a calcium-containing in-office bleaching gel. J Am Dent Assoc 2012;143(12):e81–e87. DOI: 10.14219/jada.archive.2012.0075.
  52. Acuña ED, Parreiras SO, Favoreto MW, et al. In-office bleaching with a commercial 40% hydrogen peroxide gel modified to have different pHs: color change, surface morphology, and penetration of hydrogen peroxide into the pulp chamber. J Esthet Restor Dent 2019;1–6. DOI: 10.1111/jerd.12453.
  53. Balladares L, Alegría-Acevedo LF, Montenegro-Arana A, et al. Effects of pH and application technique of in-office bleaching gels on hydrogen peroxide penetration into the pulp chamber. Oper Dent 2019;44(6):659–667. DOI: 10.2341/18-148-L.
  54. Camargo SEA, Valera MC, Camargo CHR, et al. Penetration of 38% hydrogen peroxide into the pulp chamber in bovine and human teeth submitted to office bleach technique. J Endod 2007;33(9):1074–1077. DOI: 10.1016/j.joen.2007.04.014.
  55. Benetti F, Gomes-Filho JE, Ferreira LL, et al. Hydrogen peroxide induces cell proliferation and apoptosis in pulp of rats after dental bleaching in vivo: effects of the dental bleaching in pulp. Arch Oral Biol 2017;81(6):103–109. DOI: 10.1016/j.archoralbio.2017.04.013.
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