Biological Impact of Alloplastic Bone Graft vs Bovine Xenograft and Allograft Materials in Bone Healing: An Experimental Study
Mohammed Ali Saleh Flifl, Hamdy Marzook, Mona Denewar, Heba Abo-Elfetouh Elsheikh
Allograft, Alloplast, Bone defect, Bone healing, Xenograft
Citation Information :
Flifl MA, Marzook H, Denewar M, Elsheikh HA. Biological Impact of Alloplastic Bone Graft vs Bovine Xenograft and Allograft Materials in Bone Healing: An Experimental Study. J Contemp Dent Pract 2022; 23 (5):482-491.
Aim: This study aims to compare the performance of beta-tricalcium phosphate with calcium sulfate (β-TCP/CS) vs a bovine xenograft, freeze-dried mineralized allograft, and spontaneous healing in surgically prepared bone defects in rabbit tibia.
Materials and methods: The grafting materials were implanted in three out of four standardized monocortical bony defects, 3-mm diameter and 3-mm deep, in rabbit tibia while one defect was left empty for spontaneous healing as a control group. Twelve rabbits were euthanized at 2 and 6 weeks after surgery. The bone tissue specimens were histologically evaluated using hematoxylin and eosin, Masson's trichrome and osteoprotegrin (OPG) immunohistochemical staining. Results were quantitatively evaluated.
Results: An enhancement of bone healing was noticed in the defects grafted with β-TCP/CS compared with all other groups at 2 and 6 weeks after surgery as it showed significant increase in OPG expression and a significant decrease in the amount of collagen at 6 weeks after surgery. The residual grafted particles were the least with β-TCP/CS at 6 weeks after surgery.
Conclusion: The β-TCP/CS grafting material is a promising bioactive alloplastic bone substitute as it proved to be biocompatible, osteoconductive, and bioresorbable bone substitute.
Clinical significance: The β-TCP/CS grafting material can be used for guided bone regeneration resulting in pronounced high-quality bone which aids in oral and maxillofacial reconstruction.
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