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VOLUME 23 , ISSUE 8 ( August, 2022 ) > List of Articles


Quantitative Microbial Leakage Evaluation of Restorative Materials with/without Antibacterial Primer as an Intracoronal Barrier: An Ex Vivo Study

Ambalavanan Parthasarathy, Janarthan Thangadurai, Konsam Bidya Devi, Sahana Maben, Mirza Muzaamill Baig

Keywords : Antibacterial, Clearfil, Intracoronal orifice barrier, Microleakage, Primer

Citation Information : Parthasarathy A, Thangadurai J, Devi KB, Maben S, Baig MM. Quantitative Microbial Leakage Evaluation of Restorative Materials with/without Antibacterial Primer as an Intracoronal Barrier: An Ex Vivo Study. J Contemp Dent Pract 2022; 23 (8):813-818.

DOI: 10.5005/jp-journals-10024-3357

License: CC BY-NC 4.0

Published Online: 29-11-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Aim: Aim of this research was to assess the microbial leakage of restorative materials with/without antibacterial primer as an intracoronal barrier. Materials and methods: Fifty-five extracted single-rooted teeth were included in this study. The canals were cleaned, shaped, and obturated with gutta-percha and AH plus sealer at the established working length. After removing 2 mm of coronal gutta-percha, the teeth were incubated for 24 hours. The teeth were divided into groups according to the materials used as intracoronary orifice barriers as follows: • Group I: Clearfil Protect Bond/Clearfil AP-X • Group II: Xeno IV/Clearfil AP-X • Group III: Chemflex (glass ionomer) • Group IV: Positive control (no barrier) • Group V: Negative control (no barrier and inoculated with sterile broth) Sterile 2 chambers bacterial technique was used to assess the microleakage and Enterococcus faecalis was considered as a microbial marker. The percentage of samples leaked, the time taken for leakage, and the number of colony-forming units (CFUs) in the leaked samples were calculated and analyzed statistically. Results: There was no statistically significant difference found in bacterial penetration among the three investigated materials after 120 days of use as an intracoronal orifice barrier. This study can also infer that the leaked sample from the Clearfil Protect Bond showed the least mean number of CFUs (43 CFUs) followed by Xeno IV (61 CFUs) and glass ionomer cement (GIC) (63 CFUs). Conclusion: This study concluded that all three experimental antibacterial primers performed better as intracoronal barrier. However, Clearfil Protect Bond with an antibacterial primer showed promising results as an intracoronal orifice barrier in reducing the number of bacterial leakages. Clinical significance: The significance of intracoronal orifice barriers in the success of endodontic treatment depends on the ability of the materials to prevent microleakage. This helps clinicians to provide successful antibacterial therapy against endodontic anaerobes.

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