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VOLUME 24 , ISSUE 12 ( December, 2023 ) > List of Articles

ORIGINAL RESEARCH

Assessment of Toxicity of Green Synthesized Silver Nanoparticle-coated Titanium Mini-implants with Uncoated Mini-implants: Comparison in an Animal Model Study

Swapna Sreenivasagan, Aravind Kumar Subramanian, Karthik Ganesh Mohanraj, Rajesh S Kumar

Keywords : Antimicrobial, Mini-implant, Silver nanoparticle, Systemic toxicity, Temporary anchorage device

Citation Information : Sreenivasagan S, Subramanian AK, Mohanraj KG, Kumar RS. Assessment of Toxicity of Green Synthesized Silver Nanoparticle-coated Titanium Mini-implants with Uncoated Mini-implants: Comparison in an Animal Model Study. J Contemp Dent Pract 2023; 24 (12):944-950.

DOI: 10.5005/jp-journals-10024-3577

License: CC BY-NC 4.0

Published Online: 31-01-2024

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

Aim: To assess the potential for systemic toxicity when silver nanoparticle-coated mini-implants were implanted in Wistar albino rats conducted as a comparative study in the animal model by assessing the blood biochemistry, liver and kidney function, and histology of the implanted site. Materials and methods: The surface of the mini-implant was coated with a green-mediated silver nanoparticle. Uncoated mini-implants were placed in two groups of eight Wistar albino rats, and silver nanoparticle-coated mini-implants were placed in another eight rats. The bone's general conditions, blood biochemistry assessing for ALT, AST, GPT, GOT, and histological sections using H and E stain and Masson's Trichrome stain were examined at 7, 14, and 28-day intervals. Results: The creatinine, urea, ALP, and ALT showed no signs of systemic toxicity during the 28-day follow-up period in the Wistar rats both in the test and control groups. The histological evaluation, which was conducted using HE and MTS stain, revealed osteogenesis and adequate healing of the insertion site in the group where coated mini-implant was placed. The bone sample revealed no abnormalities in the control group with uncoated mini-implants. Conclusion: Green synthesized silver nanoparticle-coated mini-implant does not cause systemic toxicity as indicated by no abnormalities in the levels of creatinine, urea, ALT, ALP, GPT, and GOT. The bone histology indicates that the coated mini-implants placed in animal bone healed with adequate osteogenesis. Clinical significance: Silver nanoparticles have potential for antimicrobial activity. Mini-implants placed as temporary anchorage devices in orthodontics often fail due to inflammation and plaque. Silver nanoparticle-coated mini-implants would reduce the risk of mini-implant failure as it would have antimicrobial potential and eliminate this cause for failure of mini-implants.


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  1. Albouy JP, Abrahamsson I, Berglundh T. Spontaneous progression of experimental peri-implantitis at implants with different surface characteristics: An experimental study in dogs. Journal of clinical periodontology 2012;39(2):182–187. DOI: 10.1111/j.1600-051X.2011.01820.x.
  2. Durán N, Durán M, de Jesus MB, et al. Antimicrobial activity mechanisms of silver nanoparticles: An overview. Nanomedicine 2016;12:789–799. DOI: 10.1016/j.nano.2015.11.016.
  3. Levard C, Hotze EM, Lowry GV, et al. Environmental transformations of silver nanoparticles: Impact on stability and toxicity. Environmental science & technology 2012;46(13):6900–6914. DOI: 10.1021/es2037405.
  4. Sreenivasagan S, Subramanian AK, Rajeshkumar S. Assessment of antimicrobial activity and cytotoxic effect of green mediated silver nanoparticles and its coating onto mini-implants. Annals of Phytomedicine 2020;9(1):207–212. DOI: 10.21276/ap.2020.9.1.27.
  5. Venugopal A, Muthuchamy N, Tejani H, et al. Incorporation of silver nanoparticles on the surface of orthodontic microimplants to achieve antimicrobial properties. The korean journal of orthodontics 2017;47(1):3–10. DOI: 10.4041/kjod.2017.47.1.3.
  6. Bottero JY, Auffan M, Rose J, et al. Manufactured metal and metal-oxide nanoparticles: properties and perturbing mechanisms of their biological activity in ecosystems. Comptes Rendus Geoscience 2011;343(2–3):168–176. DOI: 10.1016/j.crte.2011.01.001.
  7. Yang X, Gondikas AP, Marinakos SM, et al. Mechanism of silver nanoparticle toxicity is dependent on dissolved silver and surface coating in Caenorhabditis elegans. Environmental science & technology 2012;46(2):1119–1127. DOI: 10.1021/es202417t.
  8. Sengupta P. The laboratory rat: Relating its age with human's. Int J Prev Med 2013;4:624–630. PMID: 23930179.
  9. Xiu ZM, Ma J, Alvarez PJ. Differential effect of common ligands and molecular oxygen on antimicrobial activity of silver nanoparticles versus silver ions. Environmental science & technology 2011;45(20):9003–9008. DOI: 10.1021/es201918f.
  10. Loeschner K, Hadrup N, Qvortrup K, et al. Distribution of silver in rats following 28 days of repeated oral exposure to silver nanoparticles or silver acetate. Particle and fibre toxicology 2011;8:1–4. DOI: 10.1186/1743-8977-8-18.
  11. El Mahdy MM, Eldin TA, Aly HS, et al. Evaluation of hepatotoxic and genotoxic potential of silver nanoparticles in albino rats. Experimental and toxicologic pathology 2015;67(1):21–29. DOI: 10.1016/j.etp.2014.09.005.
  12. Sarhan OM, Hussein RM. Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat. International journal of nanomedicine 2014:1505–1517. DOI: 10.2147/IJN.S56729.
  13. Ahmed SM, Abdelrahman SA, Shalaby SM. Evaluating the effect of silver nanoparticles on testes of adult albino rats (histological, immunohistochemical and biochemical study). J Mol Histol 2017;48:9–27. DOI: 10.1007/s10735-016-9701-4.
  14. Tiwari DK, Jin T, Behari J. Dose-dependent in-vivo toxicity assessment of silver nanoparticle in Wistar rats. Toxicology mechanisms and methods 2011;21(1):13–24. 10.3109/15376516.2010.529184.
  15. Hassanen EI, Khalaf AA, Tohamy AF, et al. Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats. International journal of nanomedicine 2019:4723–4739. DOI: 10.2147/IJN.S207644.
  16. Kim YS, Kim JS, Cho HS, et al. Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhalation toxicology 2008;20(6):575–583. DOI: 10.1080/08958370701874663.
  17. Lee JH, Kim YS, Song KS, et al. Biopersistence of silver nanoparticles in tissues from Sprague–Dawley rats. Particle and fibre toxicology 2013;10(1):1–4. DOI: 10.1186/1743-8977-10-36.
  18. Van der Zande M, Vandebriel RJ, Van Doren E, et al. Distribution, elimination, and toxicity of silver nanoparticles and silver ions in rats after 28-day oral exposure. ACS nano 2012;6(8):7427–7442. DOI: 10.1021/nn302649p.
  19. Tang J, Xiong L, Wang S, et al. Distribution, translocation and accumulation of silver nanoparticles in rats. Journal of nanoscience and nanotechnology 2009;9(8):4924–4932. DOI: 10.1166/jnn.2009.1269.
  20. Park K, Park EJ, Chun IK, et al. Bioavailability and toxicokinetics of citrate-coated silver nanoparticles in rats. Archives of pharmacal research 2011;34:153–158. DOI: 10.1007/s12272-011-0118-z.
  21. Wen H, Dan M, Yang Y, et al. Acute toxicity and genotoxicity of silver nanoparticle in rats. PloS one 2017;12(9):e0185554. DOI: 10.1371/journal.pone.0185554.
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