The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 24 , ISSUE 6 ( June, 2023 ) > List of Articles


Estimation of Gingival Crevicular Blood as Noninvasive Method to Determine the Blood Glucose Level: A Comparative Study

Kailash Chandra Dash, Pavithra K Ramanna, Lin Jacob Varghese, Shruthi Nambiar, Avani Patel

Keywords : Diabetes mellitus, Finger-prick blood, Gingival crevicular blood, Venous blood

Citation Information : Dash KC, Ramanna PK, Varghese LJ, Nambiar S, Patel A. Estimation of Gingival Crevicular Blood as Noninvasive Method to Determine the Blood Glucose Level: A Comparative Study. J Contemp Dent Pract 2023; 24 (6):381-384.

DOI: 10.5005/jp-journals-10024-3473

License: CC BY-NC 4.0

Published Online: 28-07-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Aim: The current study's aim was to assess gingival crevicular blood as a noninvasive method to measure blood glucose levels. Materials and methods: The current study comprised a total of 50 patients who had been diagnosed with chronic periodontitis and age was ≥30 years old. The study's procedures were carried out after receiving informed consent. For finger capillary blood collection method, a sterile lancet was used to prick the finger and a drop of blood was taken, for gingival crevicular blood collection method, blood was taken from the gingival margin of the chosen site, and for venous blood collection method with the aid of a disposable syringe, a venous blood sample was taken from the patient's antecubital fossa for determining blood glucose levels. One-way analysis of variance (ANOVA) was used to examine the differences between the three methodologies’ significance, and Karl Pearson's correlation (r) was used to determine their correlation. Results: The maximum glucose level was found in venous blood (187.78 ± 18.23), followed by finger capillary blood (181.88 ± 21.67) and gingival crevicular blood (169.04 ± 11.24). And there was no significant difference between the different blood collection methods (p > 0.05). The positive significant correlation was found between gingival crevicular blood and finger capillary blood (r = 0.912, p < 0.001). Correlation with gingival crevicular blood and venous blood showed a positive correlation (r = 0.898, p < 0.001). Correlation between venous blood and finger capillary blood also showed a strong positive correlation (r = 0.988, p < 0.001). Conclusion: In conclusion, the findings of the current study suggest that blood drawn from the gingival crevicular during a clinical examination may be a great source for glucometric analysis. The gingival crevicular blood may show to be a promising technique for routine dental office screening for diabetes mellitus in periodontal patients, even if capillary/venous blood samples used for diabetes mellitus screening are the gold standard. Clinical significance: Oral health is crucial for the early detection of many systemic disorders. As a result, dentists are crucial in the screening for systemic disorders. One of the prevalent chronic disorders is diabetes. Any systemic disease that is detected early enough can avoid long-term problems.

  1. Baynest HW. Classification, pathophysiology, diagnosis and management of diabetes mellitus. J Diabetes Metab 2015;6(5):01–09.
  2. Patel C, Shah V, Dave B, et al. Comparative evaluation of the efficacy of gingival crevicular blood with finger capillary blood and venous capillary blood to assess blood glucose levels for screening of diabetes mellitus in chronic periodontitis patients: A cross-sectional study. J Clin Diagn Res 2023;17(1):ZD11–ZD15. DOI: 10.7860/JCDR/2023/59578.17223.
  3. Rees TD. Periodontal management of the patient with diabetes mellitus. Periodontol 2000 2000;23:63–72. DOI: 10.1034/j.1600-0757.2000.2230105.x.
  4. Debnath P, Govila V, Sharma M, et al. Glucometric assessment of gingival crevicular blood in diabetic and non-diabetic patients: A randomized clinical trial. J Oral Biol Craniofac Res 2015;5(1):2–6. DOI: 10.1016/j.jobcr.2014.12.004.
  5. Ramachandran A. Epidemiology of type 2 diabetes in Indians. J Indian Med Assoc 2002;100(7):425–427. PMID: 12674166.
  6. Kishore J, Kumari R, Harsha M. Gingival crevicular blood in the diagnosis of blood glucose level. Int J Contemp Med Res 2020;7(2):B1–B4.
  7. Rajesh KS, Irshana R, Arun Kumar MS, et al. Effectiveness of glucometer in screening diabetes mellitus using gingival crevicular blood. Contemp Clin Dent 2016;7(2):182–185. DOI: 10.4103/0976-237X.183072.
  8. Harris MI, Eastman RC. Early detection of undiagnosed diabetes mellitus: A US perspective. Diabetes Metab Res Rev 2000;16(4):230–236. DOI: 10.1002/1520-7560(2000)9999:9999<::aid-dmrr122>;2-w.
  9. Kost J, Mitragotri S, Gabbay RA, et al. Transdermal monitoring of glucose and other analysis using ultrasound. Nat Med 2000;6(3):347–350. DOI: 10.1038/73213.
  10. Datta S, Devraj GC. Detection of blood glucose level through gingival crevicular blood – A pilot study. J Res Med Dent Sci 2015;3(1):69–72. DOI: 10.5455/jrmds.20153115.
  11. Strauss SM, Wheeler AJ, Russell SL, et al. The potential use of gingival crevicular blood for measuring glucose to screen for diabetes: An examination based on characteristics of the blood collection site. J Periodontol 2009;80(6):907–914. DOI: 10.1902/jop.2009.080542.
  12. Kaur H, Singh B, Sharma A. Assessment of blood glucose using gingival crevicular blood in diabetic and non-diabetic patients: A chair side method. J Clin Diagn Res 2013;7(12):3066–3069. DOI: 10.7860/JCDR/2013/7705.3854.
  13. Klonoff DC. Noninvasive blood glucose monitoring. Diabetes Care 1997;20(3):433–437. DOI: 10.2337/diacare.20.3.433.
  14. Stein GM, Nebbia AA. A chairside method of diabetic screening with gingival blood. Oral Surg Oral Med Oral Pathol 1969;27(5):607–612. DOI: 10.1016/0030-4220(69)90092-9.
  15. Beikler T, Kuczek A, Petersilka G, et al. In-dental-office screening for diabetes mellitus using gingival crevicular blood. J Clin Periodontol 2002;29(3):216–218. DOI: 10.1034/j.1600-051x.2002.290306.x.
  16. Shetty N, Shankarapillai R, Mathur LK, et al. Gingival crevicular blood: As a non-invasive screening tool for diabetes mellitus in dental clinics. J Indian Soc Periodontol 2013;17(4):472–477. DOI: 10.4103/0972-124X.118319.
  17. Partheeban I, Chaly P, Priyadarshni I, et al. Evaluation of gingival blood as a minimally invasive screening tool for diabetes mellitus among 40–59-year-old adults in dental clinics: A cross-sectional study. Indian J Dent Res 2017;28(2):144–150. DOI: 10.4103/ijdr.IJDR_304_16.
  18. Banerjee S, Ganguly R, Pal TK. Gingival crevicular blood: A noninvasive pathway to determine blood glucose level in periodontal patients. Indian J Dent Sci 2017;9(4):220–224. DOI: 10.4103/IJDS.IJDS_88_17.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.