Aim: To assess the coronal and apical microleakage of mineral trioxide aggregate (MTA), Biodentine, and Bioceramic putty when used as coronal and apical seals in endodontically treated teeth. Materials and methods: The study was conducted on 90 mandibular premolar teeth. The groups were divided into two subgroups. One group assessed the coronal seal (n = 45), and the other group assessed the apical seal (n = 45). Coronal and apical 3 mm of the root filling was removed and replaced randomly with MTA, Biodentine, and Bioceramic putty in the three experimental subgroups (n = 15). All teeth were immersed in methylene blue and after incubation, the degree of dye penetration was assessed under a stereomicroscope of ×10 magnification. Statistical analysis was done using the Kruskal–Wallis test with a p-value set at 0.05. Results: The mean depth of dye penetration for coronal sealing in the MTA group was 2.91 ± 0.66, in the Biodentine group was 2.12 ± 0.50 and in the Bioceramic putty group was 1.51 ± 0.46. The mean depth of dye penetration for root-end sealing in the MTA group was 0.75 ± 0.55, in the Biodentine group was 1.94 ± 0.88 and in the Bioceramic putty group was 0.29 ± 0.20. Conclusion: Within the limitations of this study, it can be concluded that Bioceramic putty exhibited better sealing ability in both coronal and apical barriers of root canals. Clinical Significance: The sealing ability of a material vastly influences the treatment outcome of nonsurgical and surgical root therapy. The study evaluates the coronal and apical seal of three physical variants of Bioceramic material.