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VOLUME 25 , ISSUE 3 ( March, 2024 ) > List of Articles


Salivary Proteinase 3 as a Biomarker for Caries Severity in Children: A Cross-sectional Study

S Karthika, Sageena George, Anandaraj Soman, Shiana Jo, Fahanna Beegum, Mohammed A Habibullah

Keywords : Biomarker, Caries severity, Dental caries, Proteinase 3

Citation Information : Karthika S, George S, Soman A, Jo S, Beegum F, Habibullah MA. Salivary Proteinase 3 as a Biomarker for Caries Severity in Children: A Cross-sectional Study. J Contemp Dent Pract 2024; 25 (3):236-240.

DOI: 10.5005/jp-journals-10024-3648

License: CC BY-NC 4.0

Published Online: 19-04-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Aim: This study aims to evaluate the relation between salivary proteinase 3 (PR3) concentration and caries severity in children. Materials and methods: Six-to-eight-year age group children, from the Outpatient Department of Pediatric and Preventive dentistry at PMS Dental College were selected for the study. From these children, three groups each consisting of 28 children were selected according to the dental caries severity. Three groups were: (1) No Dental Caries group, (2) Low Dental Caries group with DMFT/DEFT score of 1–4, and (3) High Dental Caries group with DMFT/DEFT score of 5–15. Thus, a total of 84 children who satisfied the inclusion criteria were selected. The concentration of PR3 in saliva of the donors were analyzed using an ELISA kit. One way ANOVA was used for finding the relation of salivary PR3 concentration with caries severity. Pairwise comparison of PR3 concentration and caries severity were analyzed using post hoc Tukey test. Results: Severity of caries and concentration of salivary PR3 showed an inverse relation. As the caries severity increases there was a decrease in PR3 concentration and vice versa. Conclusion: The children with high caries severity showed lower concentration of PR3 in their saliva compared with those with lower caries severity which indicates that PR3 can be used as a biomarker for assessing caries severity and also paves way to use PR3 as a caries vaccine in future. Nowadays, interest toward noninvasive and personalized dentistry has been increased. Molecular assays using salivary biomarkers can be an effective tool in detecting the caries in earlier stages and assessing a patient's caries risk. Clinical significance: Salivary PR3 can be used as prognostic biomarker for assessing caries severity and after treatment the value of PR3 can be used as a assessment tool to confirm its relation with caries.

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  1. Byun R, Carlier JP, Jacques NA, et al. Veillonella denticariosi sp. nov., isolated from human carious dentine. Int J of Syst and Evoln Micro 2007;57:2844–2848. DOI: 10.1099/ijs.0.65096-0.
  2. Smith GA, Riedford K. Epidemiology of early childhood caries: Clinical application. J Pediatric Nurs 2013;28(4):369–373. DOI: 10.1016/j.pedn.2012.04.005.
  3. Ng MW, Ramos-Gomez F, Lieberman M, et al. Disease management of early childhood caries: ECC Collaborative Project. Int J Dent 2014;2014:327801. DOI: 10.1155/2014/327801.
  4. Ng MW, Ramos-Gomez F. Disease prevention and management of early childhood caries. J Mass Dent Soc 2012;61(3):28–32. PMID: 23311042.
  5. Ai JY, Smith B, Wong DT. Bioinformatics advances in saliva diagnostics. Int J Oral Sci 2012;4(2):85–87. DOI: 10.1038/ijos.2012.26.
  6. Garrett JR, Ekstrom J, Anderson LC. Glandular mechanism of salivary secretion Basel: Karger-Switzerland; 1998. DOI: 10.1159/isbn.978-3-318-00266-9.
  7. Chiappin S, Antonelli G, Gatti R, et al. Saliva specimen: A new laboratory tool for diagnostic and basic investigation. Clin Chim Acta 2007;383:30–40. DOI: 10.1016/j.cca.2007.04.011.
  8. Korkmaz B, Lesner A, Letast S, et al. Neutrophil proteinase 3 and dipeptidyl peptidase I (cathepsin C) as pharmacological targets in granulomatosis with polyangiitis (Wegener granulomatosis). Semin Immunopathol 2013;35(4):411–421. DOI: 10.1007/s00281-013- 0362-z.
  9. Guarino C, Legowska M, Epinette C, et al. New selective peptidyl di(chlorophenyl) phosphonate esters for visualizing and blocking neutrophil proteinase 3 in human diseases. J Biol Chem 2014;289(46):31777–31791. DOI: 10.1074/jbc.M114.591339.
  10. Schrijver G, Schalkwijk J, Robben JC, et al. Antiglomerular basement membrane nephritis in beige mice. Deficiency of leukocytic neutral proteinases prevents the induction of albuminuria in the heterologous phase. J Exp Med 1989;169(4):1435–1448. DOI: 10.1084/jem.169.4.1435.
  11. Liu Z, Shapiro SD, Zhou X, et al. A critical role for neutrophil elastase in experimental bullous pemphigoid. J Clin Invest 2000;105(1):113–123. DOI: 10.1172/JCI3693.
  12. Yang TY, Zhou WJ, Du Y, et al. Role of saliva proteinase 3 in dental caries. Int J Oral Sci 2015;7(3):174–178. DOI: 10.1038/ijos.2015.8.
  13. Moses J, Rangeeth BN, Gurunathan D. Prevalence of dental caries, socio-economic old school going children of chid ambaram status and treatment needs among 5- to 15-year-old school going children Of Chidambaram. J Clin Dign Res 2011;5:146–151. DOI:
  14. Malamud D, Isaac R, Chavez R. Saliva as a diagnostic fluid. Dent Clin North Am 2011;55(1):59–178. DOI:
  15. Livny A, Assali R, Sgan-Cohen H. Early childhood caries among a Bedouin community residing in the eastern outskirts of Jerusalem. BMC Public Health 2007;7:167. DOI: 10.1186/1471-2458-7-167.
  16. Gilks CF, Balbirnie E. Testing sputum samples for HIV antibodies using a rapid, saliva-card test system. Ann Trop Med Parasitol 1997;91: 115–117. DOI: 10.1080/00034983.1997.11813119.
  17. Lima DP, Diniz DG, Moimaz SA, et al. Saliva: Reflection of the body. Int J Infect Dis 2010;14(3):e184–e188. DOI: 10.1016/j.ijid.2009.04.022.
  18. Sánchez-García S, Gutiérrez-Venegas G, Juárez-Cedillo T, et al. Simplified caries risk test in stimulated saliva from elderly patients. Gerodontology 2008;25:26–33. DOI: 10.1111/j.1741-2358.2007.00184.x.
  19. Brinkmann V, Reichard U, Goosmann C, et al. Neutrophil extracellular traps kill bacteria. Science 2004;303(5663):1532–1535. DOI: 10.1126/science.1092385.
  20. Helmerhorst EJ, Oppenheim FG. Saliva: A dynamic proteome. J Dent Res 2007;86(8):680–693. DOI: 10.1177/154405910708600802.
  21. Alamoudi A, Alamoudi R, Gazzaz Y, et al. Role of salivary biomarkers in diagnosis and detection of dental caries: A systematic review. diagnostics (Basel) 2022;12(12):3080. DOI: 10.3390/diagnostics12123080.
  22. Biria M, Satari M, Iranparvar P, et al. Relationship between salivary concentration of PR3 and interleukins-8 and severe childhood caries. Dent Med Problm 2023;60(4):577–582. DOI: 10.17219/dmp/132517.
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