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VOLUME 25 , ISSUE 4 ( April, 2024 ) > List of Articles

ORIGINAL RESEARCH

Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study

Hamed A Alshawkani, Mohamed Mansy, Mahmoud Al Ankily, Mohamed Shamel

Keywords : Bioceramic, Dental pulp stem cells, Odontogenic genes, Photobiomodulation, Vital pulp therapy

Citation Information : Alshawkani HA, Mansy M, Al Ankily M, Shamel M. Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study. J Contemp Dent Pract 2024; 25 (4):313-319.

DOI: 10.5005/jp-journals-10024-3676

License: CC BY-NC 4.0

Published Online: 14-06-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Abstract

Aims: This study aims to assess the synergistic effect of utilizing a bioceramic sealer, NeoPutty, with photobiomodulation (PBM) on dental pulp stem cells (DPSCs) for odontogenesis. Materials and methods: Dental pulp stem cells were collected from 10 premolars extracted from healthy individuals. Dental pulp stem cells were characterized using an inverted-phase microscope to detect cell shape and flow cytometry to detect stem cell-specific surface antigens. Three experimental groups were examined: the NP group, the PBM group, and the combined NP and PBM group. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) experiment was conducted to assess the viability of DPSCs. The odontogenic differentiation potential was analyzed using Alizarin red staining, RT-qPCR analysis of odontogenic genes DMP-1, DSPP, and alkaline phosphatase (ALP), and western blot analysis for detecting BMP-2 and RUNX-2 protein expression. An analysis of variance (ANOVA) followed by a post hoc t-test was employed to examine and compare the mean values of the results. Results: The study showed a notable rise in cell viability when NP and PBM were used together. Odontogenic gene expression and the protein expression of BMP-2 and RUNX-2 were notably increased in the combined group. The combined effect of NeoPutty and PBM was significant in enhancing the odontogenic differentiation capability of DPSCs. Conclusion: The synergistic effect of NeoPutty and PBM produced the most positive effect on the cytocompatibility and odontogenic differentiation potential of DPSCs. Clinical significance: Creating innovative regenerative treatments to efficiently and durably repair injured dental tissues.

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