Aim: This study intended to comprehend the effects of injectable platelet-rich fibrin (i-PRF) on anchor loss and space closure rates during the retraction phase of orthodontic treatment. Materials and methods: Twenty-four participants with malocclusion, necessitating extractions and space closure during orthodontic treatment, were enrolled and divided into two groups (n = 12 participants) group A: the experimental group was administered i-PRF on the maxilla/mandible, while group B: the control group did not. Measurements of the rate of space closure, anchor loss, and salivary enzyme activity were done before retraction (T0), after three weeks (T1), after six weeks (T2), and after nine weeks (T3). The Mann–Whitney and the independent Student's t-test were used to compare continuous variables among groups. Results: Four participants were lost to follow-up in each group with eight participants remaining in the respective groups. The rate of space closure in the maxillary arch was 1.4 ± 1.9 mm at T3 intervals with the baseline value (T0) 10.8 ± 3.01 mm, and the rate of anchor loss was 0.57 mm at T3 intervals for group A. In group B, space closure at T0 was 11.1 ± 2.0 mm and 4.9 ± 1.5 mm at T3 while anchor loss of 0.57 mm at T3 intervals, respectively. In the mandibular arch, the rate of space closure was 2.6 ± 2.0 mm at T3 intervals, with the baseline value (T0) 9.5 ± 2.5 mm, and the rate of anchor loss was 0.325 mm at T3 for group A. In group B, space closure at T0 (baseline) were 10.0 ± 2.7 mm and 4.7 ± 2.3 at T3 mm, and anchor loss was 0.37 mm at T3 intervals, respectively. Space closure rate in the maxilla is significant statistically at intervals T2 and T3 and in the mandible is significant statistically at intervals T2, the anchor loss in both the arch is statistically insignificant. Conclusion: In both maxillary and mandibular arches, the experimental group showed accelerated tooth movement compared to the control group, although statistical significance was not achieved in the mandible. There were no apparent differences in anchor loss between the two groups. Clinical significance: The use of i-PRF in orthodontic treatment significantly enhances the rate of space closure and reduces the overall treatment. Injectable platelet-rich fibrin can be a safe adjunct to orthodontic treatment, providing benefits without compromising anchorage stability and aiming to optimize orthodontic treatment efficiency.