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VOLUME 25 , ISSUE 9 ( September, 2024 ) > List of Articles

ORIGINAL RESEARCH

Surface Modifications and Antifungal Efficacy of Origanum Oil Incorporation in Denture-based Materials: An In Vitro Study

Maria ÁL Feitosa, Victório Poletto-Neto, Tamires T Maske, Rogerio A Freitag, Rafael G Lund, Tatiana Pereira-Cenci

Keywords : Acrylic resin, Biofilm model, Candidiasis, Denture liner, Origanum oil

Citation Information : Feitosa MÁ, Poletto-Neto V, Maske TT, Freitag RA, Lund RG, Pereira-Cenci T. Surface Modifications and Antifungal Efficacy of Origanum Oil Incorporation in Denture-based Materials: An In Vitro Study. J Contemp Dent Pract 2024; 25 (9):878-884.

DOI: 10.5005/jp-journals-10024-3760

License: CC BY-NC 4.0

Published Online: 20-12-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Abstract

Aim: This study aimed to assess if the addition of origanum oil to denture materials could decrease microorganisms counts and biofilm formation without changing their mechanical/surface properties. Materials and methods: A total of 66 resilient denture liner discs (SoftConfort, Dencril Comércio de Plásticos Ltda, SP, Brazil) were prepared with fixed dimensions of 10 × 3 mm for biofilm assay (n = 36) and 12 × 2 mm for sorption-solubility tests (n = 30) containing three oil concentrations – 0, 2.5 and 5%, thereby n = 12 per each group samples for biofilm assay and n = 10 per each group for sorption-solubility test respectively. While the microcosm biofilms of Streptococcus mutans, Candida species, and total microorganisms formed on denture liner were counted and expressed as colony-forming units per disc surface area, the water sorption (WS) and solubility (SL), was calculated by weighing the samples with an analytical balance at different intervals after storing them in a desiccator and distilled water alternatively. Data was recorded and statistically analyzed. Results: Surface roughness increased in all groups after biofilm formation (p < 0.001), with the most significant change observed in the 2.5% oil group. The tested oil concentrations did not result in sorption changes, but a 5% oil concentration resulted in higher solubility (p < 0.001). A reduction of total microorganisms and S. mutans was seen after 24 hours for all concentrations (p < 0.05). No significant reduction was found for Candida (C.) albicans after 24 hours, whilst 2.5% oil concentration presented lower counts of C. albicans in comparison to the 5% group after 24 hours (p < 0.05). Conclusions: Incorporating 2.5% origanum essential oil into resilient denture liners seems to reduce microorganisms count in a complex biofilm model. These results need to be confirmed in future studies. Clinical significance: The addition of natural products like origanum oil into denture-based materials can help manage biofilm onset and development while offering a simple and effective approach for maintaining denture hygiene. This strategy enhances the antimicrobial properties of denture liners without significantly altering their mechanical and surface characteristics, potentially improving patient outcomes.

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  1. Qiu J, Roza MP, Colli KG, et al. Candida-associated denture stomatitis: Clinical, epidemiological, and microbiological features. Braz J Microbiol 2023;54(2):841–848. DOI: 10.1007/s42770-023-00952-0.
  2. Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont 2011;20(4):251–260. DOI: 10.1111/j.1532-849X.2011.00698.x.
  3. Bellon-Fontaine MN, Mozes N, van der Mei HC, et al. A comparison of thermodynamic approaches to predict the adhesion of dairy microorganisms to solid substrata. Cell Biophys 1990;17(1):93–106. DOI: 10.1007/BF02989805.
  4. Busscher HJ, Cowan MM, van der Mei HC. On the relative importance of specific and non-specific approaches to oral microbial adhesion. FEMS Microbiol Rev 1992;8(3–4):199–210. DOI: 10.1111/j.1574-6968.1992.tb04988.x.
  5. Sun X, Cao Z, Yeh CK, et al. Antifungal activity, biofilm-controlling effect, and biocompatibility of poly(N-vinyl-2-pyrrolidinone)-grafted denture materials. Colloids Surf B Biointerfaces 2013;110:96–104. DOI: 10.1016/j.colsurfb.2013.04.043.
  6. Holban AM, Farcasiu C, Andrei OC, et al. Surface modification to modulate microbial biofilms—Applications in dental medicine. Materials 2021;14(22):6994. DOI: 10.3390/ma14226994.
  7. Mendonça e Bertolini Md, Cavalcanti YW, Bordin D, et al. Candida albicans biofilms and MMA surface treatment influence the adhesion of soft denture liners to PMMA resin. Braz Oral Res 2013;28(1):61–66. DOI: 10.1590/S1806-83242013005000025.
  8. Machado AL, Giampaolo ET, Vergani CE, et al. Weight loss and changes in surface roughness of denture base and reline materials after simulated toothbrushing in vitro. Gerodontology 2012;29(2):e121–e127. DOI: 10.1111/j.1741-2358.2010.00422.x.
  9. Urban VM, De Souza RF, Galvao Arrais CA, et al. Effect of the association of nystatin with a tissue conditioner on its ultimate tensile strength. J Prosthodont 2006;15(5):295–299. DOI: 10.1111/j.1532-849X.2006.00130.x.
  10. Catalán A, Pacheco JG, Martínez A, et al. In vitro and in vivo activity of Melaleuca alternifolia mixed with tissue conditioner on Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105(3):327–332. DOI: 10.1016/j.tripleo.2007.08.025.
  11. Arbeláez MIA, Vergani CE, Barbugli PA, et al. Long-term effect of daily chemical disinfection on surface topography and Candida albicans biofilm formation on denture base and reline acrylic resins. Oral Health Prev Dent 2020;18(1):999–1010. DOI: 10.3290/j.ohpd.a45521.
  12. Zhang K, Zhang S, Shi Y, et al. Effects of disinfectants on physical properties of denture base resins: A systematic review and meta-analysis. J Prosthet Dent 2024;131(5):841–858. DOI: 10.1016/j.prosdent.2022.03.020.
  13. Kumar PS. The influence of Azadirachta indica, Melaleuca alternifolia, and Cocos nucifera on Candida albicans strain in tissue conditioner at varying time intervals. J Indian Prosthodont Soc 2020;20(2):171–179. DOI: 10.4103/jips.jips_366_19.
  14. Barreto JO, do Nascimento FBSA, Fonseca SG da C, et al. Microbiological evaluation of an experimental denture cleanser containing essential oil of Lippia sidoides. Biofouling 2021;37(1):117–130. DOI: 10.1080/08927014.2021.1885649.
  15. Silva PM, Chappuis Chocano AP, Venante HS, et al. Beneficial effects of three natural products for the treatment of denture stomatitis: A randomized clinical trial. Archives of Dental Science/Arquivos em Odontologia. 2021;57:141–148. DOI: 10.35699/2178-1990.2021. 25765.
  16. Niculescu AG, Grumezescu AM. Natural compounds for preventing ear, nose, and throat-related oral infections. Plants 2021;10(9):1847. DOI: 10.3390/plants10091847.
  17. Mohsen L, Jaber H, Kamel WM. Antibacterial activity of the essential oil isolated from Origanum vulgare L.(Lamiaceae) against multi-drug resistant bacteria. International Journal of Drug Delivery Technology 2022;12(1):81–84. DOI: 10.25258/ijddt.12.1.15.
  18. Sharifi-Rad M, Berkay Yılmaz Y, Antika G, et al. Phytochemical constituents, biological activities, and health-promoting effects of the genus Origanum. Phytother Res 2021;35(1):95–121. DOI: 10.1002/ptr.6785.
  19. Escobar A, Perez M, Romanelli G, et al. Thymol bioactivity: A review focusing on practical applications. Arab J Chem 2020;13(12):9243–9269. DOI: 10.1016/j.arabjc.2020.11.009.
  20. Imran M, Aslam M, Alsagaby SA, et al. Therapeutic application of carvacrol: A comprehensive review. Food Sci Nutri 2022;10(11):3544–3561. DOI: 10.1002/fsn3.2994.
  21. Chami N, Chami F, Bennis S, et al. Antifungal treatment with carvacrol and eugenol of oral candidiasis in immunosuppressed rats. Braz J Infect Dis 2004;8(3):217–226. DOI: 10.1590/S1413-86702004000300005.
  22. Guo N, Liu J, Wu X, et al. Antifungal activity of thymol against clinical isolates of fluconazole-sensitive and-resistant Candida albicans. J Med Microbiol 2009;58(8):1074–1079. DOI: 10.1099/jmm.0.008052-0.
  23. Zhang Z, Wang W, Li J, et al. Carvacrol: Antibacterial activity, bone repair, and prevention and treatment in oral diseases. Chinese Journal of Tissue Engineering Research 2022;26(26):4252–4257. DOI: 10.12307/2022.831.
  24. Brondani LP, da Silva Neto TA, Freitag RA, et al. Evaluation of anti-enzyme properties of Origanum vulgare essential oil against oral Candida albicans. J Mycol Med 2018;28(1):94–100. DOI: 10.1016/j.mycmed.2017.12.001.
  25. Najafloo R, Behyari M, Imani R, et al. A mini-review of Thymol incorporated materials: Applications in antibacterial wound dressing. Journal of Drug Delivery Science and Technology 2020;60:101904. DOI: 10.1016/j.jddst.2020.101904.
  26. Srivatstava A, Ginjupalli K, Perampalli NU, et al. Evaluation of the properties of a tissue conditioner containing origanum oil as an antifungal additive. J Prosthet Dent 2013;110(4):313–319. DOI: 10.1016/S0022-3913(13)60381-9.
  27. Volety S, Shetty PP, Kumar K, et al. Antifungal effects of herbal extracts and fluconazole on heat-polymerized acrylic denture base resin as denture cleanser: An in vitro study. J Contemp Dent Pract 2021;22(2):162–165. DOI: 10.5005/jp-journals-10024-3042.
  28. Eltamimi K, Mohamed S. Antifungal action of thyme and basil extracts on different denture base materials. Egypt Dent J 2021;67(4):3643–3650. DOI: 10.21608/EDJ.2021.91647.1760.
  29. Blanc AR, Sortino MA, Butassi E, et al. Synergistic effects of Thymus vulgaris essential oil in combination with antifungal agents and inhibition of virulence factors of Candida albicans. Phytomed Plus 2023;3(4):100481. DOI: 10.1016/j.phyplu.2023.100481.
  30. Filoche SK, Soma KJ, Sissons CH. Caries-related plaque microcosm biofilms developed in microplates. Oral Microbiol Immunol 2007;22(2):73–79. DOI: 10.1111/j.1399-302X.2007.00323.x.
  31. De Moraes AP, Barwaldt CK, Nunes TZ, et al. Effect of triazine derivative added to denture materials on a microcosm biofilm model. J Biomed Mater Res B Appl Biomater 2012;100B(5):1328–1333. DOI: 10.1002/jbm.b.32699.
  32. Luo G, Samaranayake LP. Candida glabrata, an emerging fungal pathogen, exhibits superior relative cell surface hydrophobicity and adhesion to denture acrylic surfaces compared with Candida albicans. APMIS 2002;110(9):601–610. DOI: 10.1034/j.1600-0463.2002.1100902.x.
  33. Oniga I, Pus C, Silaghi-Dumitrescu R, et al. Origanum vulgare ssp. vulgare: Chemical composition and biological studies. Molecules 2018;23(8):2077. DOI: 10.3390/molecules23082077.
  34. Zinno P, Guantario B, Lombardi G, et al. Chemical composition and biological activities of essential oils from Origanum vulgare genotypes belonging to the carvacrol and thymol chemotypes. Plants 2023;12(6):1344. DOI: 10.3390/plants12061344.
  35. Alqutaibi A, Baik A, Almuzaini S, et al. Polymeric denture base materials: A review. Polymers 2023;15(15):3258. DOI: 10.3390/polym15153258.
  36. Susewind S, Lang R, Hahnel S. Biofilm formation and Candida albicans morphology on the surface of denture base materials. Mycoses 2015;58(12):719–727. DOI: 10.1111/myc.12420.
  37. Valentini F, Luz MS, Boscato N, et al. Surface roughness changes in denture liners in denture stomatitis patients. Int J Prosthodont 2017;30(6):561–564. DOI: 10.11607/ijp.5108.
  38. Ponde NO, Lortal L, Ramage G, et al. Candida albicans biofilms and polymicrobial interactions. Crit Rev Microbiol 2021;47(1):91–111. DOI: 10.1080/1040841X.2020.1843400.
  39. Pereira-Cenci T, Del Bel Cury AA, Crielaard W, et al. Development of Candida-associated denture stomatitis: New insights. J Appl Oral Sci 2008;16(2):86–94. DOI: 10.1590/S1678-775720080002 00002.
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