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VOLUME 8 , ISSUE 7 ( November, 2007 ) > List of Articles


Effect of Water Storage on Bond Strength of Self-etching Adhesives to Dentin

Rubens Nazareno Garcia, Mario Fernando de Goes, Marcelo Giannini

Citation Information : Garcia RN, de Goes MF, Giannini M. Effect of Water Storage on Bond Strength of Self-etching Adhesives to Dentin. J Contemp Dent Pract 2007; 8 (7):46-53.

DOI: 10.5005/jcdp-8-7-46

License: CC BY-NC 3.0

Published Online: 01-01-2009

Copyright Statement:  Copyright © 2007; The Author(s).



The objective of this study was to evaluate the bond strength of self-etching adhesive systems one week and one year after storage in water.

Methods and Materials

Fragments from the buccal surfaces of 45 bovine teeth were prepared (12 mm in length X 5 mm in width X 1.0 mm in thickness). Dentin surfaces were wet-abraded with 600-grit SiC paper to create a standardized smear layer. Samples were randomly assigned to 18 experimental groups (n=5), according to nine adhesive systems tested (Single Bond; Adper Prompt L-Pop; iBond; One-Up Bond F; Xeno III; Clearfil SE Bond; Optibond Solo Plus SE; Tyrian SPE/One-Step Plus; and UniFil Bond) and two waterstorage times (one week and one year). Adhesives were applied according to the manufacturers’ instructions. Z250 composite was applied into the molds to fill up the internal diameter volume of a Tygon tubing mold (1.0 mm high / 0.7 mm internal diameter). Micro-shear bond strengths were determined using an apparatus attached to an Instron Universal Testing Machine (0.5 mm/min). Data were statistically analyzed using a twoway analysis of variance (ANOVA) and the Tukey's test (5%).


One year after water storage the dentin bond strength of all adhesive systems reduced significantly, except for One-Up Bond F.


Water-storage time decreased the bond strength for most dentin bonding agents tested.


Garcia RN, de Goes MF, Giannini M. Effect of Water Storage on Bond Strength of Self-etching Adhesives to Dentin. J Contemp Dent Pract 2007 November; (8)7:046-053.

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  1. Self-etching dentin primers containing Phenyl-P. J Dent Res. 1994;73:1088-95.
  2. Bonding to ground dentin by a phenyl-P self-etching primer. J Dent Res. 1994;73:1212-20.
  3. Composition, wetting properties and bond strength with dentin of 6 new dentin adhesives. Dent Mater. 1985;1:170-6.
  4. The four generations of dentin bonding. Am J Dent. 1995;8:88-92.
  5. Current concepts on adhesion to dentin. Crit Rev Oral Biol Med. 1997;8:306-35.
  6. Hybridization of dental hard tissues. 1st ed. Tokyo : Quintessence Publishing Co; 1998.
  7. Adhesion to enamel and dentin: currents status and future challenges. Oper Dent. 2003;28:215-35.
  8. A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res. 2005;84:118-32.
  9. Agressiveness of self-etch adhesives on unground enamel. Oper Dent. 2004;29:309-16.
  10. Single-step adhesives are permeable membranes. J Dent. 2002;30:371-82.
  11. Have dentin adhesives become too hydrophilic? J Can Dent Assoc. 2003;69:726-31.
  12. An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact dentin. J Adhes Dent. 2000;2:83-98.
  13. Aggressiveness of contemporary self-etching systems. I: Depth of penetration beyond dentin smear layers. Dent Mater. 2001;17:296-308.
  14. Effect of resin hydrophilicity and water storage on resin strength. Biomaterials. 2004;25:5789-96.
  15. Monomer solvent phase separation in one-step self-etch adhesives. J Dent Res. 2005;84:183-8.
  16. Dentin/enamel adhesives: review of the literature. Pediatr Dent. 2002; 24:456-61.
  17. The effect of organic solvents on one-bottle adhesives bond strength to enamel and dentin. Oper Dent. 2003;28:689-94.
  18. Micro-shear bond strength of dual-cured resin cement to glass ceramics. Dent Mat. 2002;8:380-8.
  19. Long-term durability of dentin bonds made with a self-etching primer, in vivo. J Dent Res. 1999;78:906-11.
  20. In vivo degradation of resin dentin bonds in human over 1 to 3 years. J Dent Res. 2000;79:1385-91.
  21. Six-month storage-time evaluation of one-bottle adhesive systems to dentin. J Esthet Restor Dent. 2003;15:43-8.
  22. Ultramorphological analysis of resin-dentin interfaces produced with water-based single-step and two-step adhesives: nanoleakage expression. J Biomed Mater Res. 2004:71(B):90-8.
  23. Effects of pre- and post-bonding hydration on bond strength to dentin. J Adhes Dent. 2004;6:13-7.
  24. Effects of HEMA on water evaporation from water-HEMA mixtures. Dent Mater. 1998;14:6-10.
  25. Solvation of dried matrix by water and other polar solvents. Am J Dent. 2002;15:97-102.
  26. Water sorption of several bonding resins. Am J Dent. 1999;12:295-8.
  27. Technique sensitivity in bonding to vital, acid-etched dentin. Oper Dent. 2003;28:3-8.
  28. Aging affects two modes of nanoleakage expression in bonded dentin. J Dent Res. 2003;82:537-41.
  29. Correlation of dentin bond durability with water absorption of bonding layer. Dent Mater J. 1999;18:11-8.
  30. Importance of water sorption and solubility studies for couple bonding agent/resin-based filling material. Oper Dent. 2004;29:669-76.
  31. In vivo and in vitro permeability of One-step self-etch adhesives. J Dent Res. 2004;83:459-64.
  32. Interfacial micromorphology and shear bond strength of singlebottle primer/adhesives. Dent Mater. 1997;13:316-24.
  33. Bovine teeth as possible human teeth substitutes. J Dent Res. 1983;62:1076-81.
  34. Comparision of microtensile bond strength of human, bovine and porcine teeth. J Adhes Dent. 2004; 6:117-21.
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