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VOLUME 9 , ISSUE 5 ( July, 2008 ) > List of Articles


Smear Layer Removal and Collagen Fiber Exposure Using Tetracycline Hydrochloride Conditioning

Andrea Abi Rached Dantas, Eduardo de Paula Ishi, Luiz Henrique Carvalho Batista, Mirian Aparecida Onofre, José Eduardo C. Sampaio

Citation Information : Dantas AA, de Paula Ishi E, Batista LH, Onofre MA, Sampaio JE. Smear Layer Removal and Collagen Fiber Exposure Using Tetracycline Hydrochloride Conditioning. J Contemp Dent Pract 2008; 9 (5):25-33.

DOI: 10.5005/jcdp-9-5-25

License: CC BY-NC 3.0

Published Online: 01-01-2010

Copyright Statement:  Copyright © 2008; The Author(s).



Smear layer removal and collagen fiber exposure may improve periodontal treatment and regeneration. This in vitro study assessed smear layer removal and collagen fiber exposure after tetracycline hydrochloride (TTC) application on root surfaces using scanning electron microscopy (SEM).

Methods and Materials

Root cementum was removed with diamond burs followed by scaling and root planning. Four hundred fifty samples were divided into ten groups: a control (saline application) and nine different TTC concentrations were applied at doses of 10, 25, 50, 75, 100, 125, 150, 200, and 250 mg/ml. The TTC application was performed in all groups in three different ways (passive, brushing, and burnishing) and at three different periods of conditioning (1, 2, and 3 minutes). A previously trained, calibrated, and blind examiner evaluated photomicrographs of the samples using Sampaio's index (2005). Statistical analysis was performed using the Kruskal-Wallis’ and Dunn's tests.


The concentrations of 50 mg/mL and 75 mg/mL applied by burnishing were the most effective in smear layer removal and collagen fiber exposure. Both the passive mode of application (p=0.0001) and 1 minute period of application (p=0.002) were the least effective.


The concentrations of 50 mg/mL and 75 mg/mL applied by burnishing during 2 or 3 minutes were the most effective.

Clinical Significance

These parameters may be applied in periodontal procedures involving TTC root conditioning to optimize results.


Ishi EP, Dantas AAR, Batista LHC, Onofre MA, Sampaio JEC. Smear Layer Removal and Collagen Fiber Exposure Using Tetracycline Hydrochloride Conditioning. J Contemp Dent Pract 2008 July; (9)5:025-033.

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