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VOLUME 9 , ISSUE 6 ( September, 2008 ) > List of Articles

RESEARCH ARTICLE

Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells

Fatih Ozan, Zübeyde Akin Polat, Bektaş Tepe, Kürşat Er

Citation Information : Ozan F, Polat ZA, Tepe B, Er K. Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells. J Contemp Dent Pract 2008; 9 (6):17-24.

DOI: 10.5005/jcdp-9-6-17

License: CC BY-NC 3.0

Published Online: 01-09-2008

Copyright Statement:  Copyright © 2008; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

The purpose of this study was to determine the ability of Salvia officinalis (S. officinalis) extracts to serve as a storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth.

Methods and Materials

PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultures were subjected to 4, 2.5, 1.5, and 0.5% S. officinalis solutions, Hank's balanced salt solution (HBSS), phosphate buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37°C for 1, 3, 6, 12 or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was performed by one-way analysis of variance (ANOVA) complemented by the Tukey's test. The level of significance was 5% (p< 0.05).

Results

The results showed 2.5% S. officinalis was a more effective storage medium than the other experimental solutions (p<0.05). Only at 1 hour and 3 hours was there found similar effect between 2.5% S. officinalis and HBSS. At 24 hours, 2.5% S. officinalis was found to be significantly better than the other solutions tested.

Conclusion

S. officinalis can be recommended as a suitable transport medium for avulsed teeth.

Clinical Significance

The findings of this study support the use of S. officinalis as another option for clinicians to use to store and transport avulsed teeth until reimplantation procedures can be done.

Citation

Özan F, Polat ZA, Tepe B, Er K. Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells. J Contemp Dent Pract 2008 September; (9)6:017-024.


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  1. Traumatic injuries. In: Cohen S, Burns RC, eds. Pathways of the pulp. 7th ed. St. Louis: CV Mosby, 1998.
  2. Recommended guidelines for the treatment of the avulsed permanent tooth. Chicago: American Association of Endodontists, 1995.
  3. Determination of periodontal ligament cell viability in long shelf-life milk. J Endod 2000; 26:699-702.
  4. Effect of propolis on survival of periodontal ligament cells: new storage media for avulsed teeth. J Endod 2007; 33:570-3.
  5. Flora of Turkey and the East Aegean Islands. Vol. 11 (supplement-II). Edinburgh University Press, Edinburgh, 2000.
  6. Herbal medicines. A Guide for health-care professionals. London: The pharmaceutical Press, 1996, pp. 231.
  7. Active-oxygen scavenging activity of plant extracts. Biol Pharm Bull 1995; 18:162-6.
  8. Protective effects of the aerial parts of Salvia officinalis, Melissa officinalis and Lavandula angustifolia and their constituents against enzyme-dependent and enzyme-independent lipid peroxidation. Planta Med 1999; 65:576-8.
  9. Antioxidant constituents of Rosmarinus officinalis and Salvia officinalis. IV. Determination of carnosic acid in different foodstuffs. Z Lebensm Unters Forsch 1995; 201:548-50.
  10. Antimicrobial, cytotoxic, and antiviral activities of Salvia fructicosa essential oil. J Agric Food Chem 1997; 45:3197-201.
  11. Antiviral diterpenes from Salvia officinalis. Phytochemistry 1994; 35:539-41.
  12. Antitumor Agents. 239. Isolation, structure elucidation, total synthesis, and anti-breast cancer activity of neotanshinlactone from Salvia miltiorrhiza. J Med Chem 2004; 47:5816-9.
  13. Antiinflammatory and ulcerogenic activities of Salvia triloba extracts. Fitoterapia 2006; 77:333-5.
  14. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem 2005;90:333–40.
  15. Oral and Maxillofacial Surgery Volume 3: Trauma. WB Saunders Co. Philadelphia, London, 2000.
  16. Effect of extra alveolar period and storage media upon periodontal and pulpla healing after replantation of mature permanent incicors in monkeys. Int J Oral Surg 1981; 10:43-53.
  17. Avulsed permanent teeth: a review of the literature and treatment guidelines. Endod Dent Traumatol 1997; 13:153-63.
  18. The effect of limited drying or removal of the periodontal ligament. Periodontal healing after replantation of mature permanent incisors in monkeys. Acta Odontol Scand 1981; 39:1-13.
  19. Relationship between surface and inflammatory resorption and changes in the pulp after replantation of permanent incisors in monkeys. J Endod 1981; 7:294-301.
  20. Replantation of 400 avulsed permanent incisors. Part 4. Factors related to periodontal ligament healing. Endod Dent Traumatol 1995; 11:76-89.
  21. Inflammatory and replacement resorption in reimplanted permanent incisor teeth: a study of the characteristics of 84 teeth. Endod Dent Traumatol 1999; 15:269-72.
  22. Variations in the presenting and treatment features in reimplanted permanent incisors in children and their effect on the prevalence of root resorption. Br Dent J 2000; 189:263-6.
  23. A prospective study of factors affecting survival of replanted permanent incisors in children. Int J Paediatr Dent 2000; 10:200-5.
  24. Human periodontal ligament cell viability in milk and milk substitutes. J Endod 2003; 29:184-6.
  25. Determination of periodontal ligament cell viability in long shelf-life milk. J Endod 2000; 26:699-702.
  26. Atlas of replantation and transplantation of teeth. Philadelphia, WB Saunders, 1992;242-56.
  27. Preserving avulsed teeth for replantation. JADA 1992; 123:80-8.
  28. Comparison of various transport media on human periodontal ligament cell viability. J Endod 1997; 23:676-79.
  29. Vitality of human lip fibroblasts in milk, hanks balanced salt solution and viaspan storage media. Endod Dent Traumatol 1991; 7:69-72.
  30. Oxygen tension regulates osteoblast function. Am J Orthod Dentofacil Orthop 1994; 105:457-63.
  31. Role of the endothelial cell in osteoclast control: new perspectives. Bone 1993; 14:97-102.
  32. Effect of catalase supplementation in storage media for avulsed teeth. Dent Traumatol 2003; 19:103-8.
  33. editors. Food phytochemicals for cancer prevention II: Chemical and Antioxidative Effects of Phenolic Compounds from Licorice, Tea and Compositae and Labiateae Herbs. Washington, DC: American Chemical Society; 1994, 132-43.
  34. Elucidation of the chemical structures of natural antioxidants isolated from rosemary. J Am Oil Chem Soc 1982; 59:339-45.
  35. Spices as antioxidants. Trends Food Sci Tech 1995; 6:271-7.
  36. Salvianolic acid L, a potent phenolic antioxidant from Salvia officinalis. Tetrahedron Letters 2001; 42:8223-5.
  37. The effect of systemic tetracycline on resorption of dried replanted dogs’ teeth. Endod Dent Traumatol 1998; 14:127-32.
  38. Effect of systemic tetracycline and amoxicillin on inflammatory root resorption of replanted dogs’ teeth. Endod Dent Traumatol 1998:14:216–220.
  39. Effect of topical application of doxycycline on pulp revascularization and periodontal healing in reimplanted monkey incisors. Endod Dent Traumatol 1990; 6:170-6.
  40. Effect of stannous fluoride and tetracycline on periodontal repair after delayed tooth replantation in dogs. Scand J Dent Res 1992; 100:200-3.
  41. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agric 1998; 77:140-6.
  42. Composition of the extract in dichloromethane of the aerial parts of a Spanish wild growing plant Thymus vulgaris L. Flavour Fragrance J 1998; 13:259-62.
  43. The in vitro antibacterial activity of Turkish plants. J Ethnopharm 1999;67:79-86.
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