Comparison of the Antibacterial Activity of Different Self-etching Primers and Adhesives
Yonca Korkmaz, Nuray Attar, Meral Ozalp
Citation Information :
Korkmaz Y, Attar N, Ozalp M. Comparison of the Antibacterial Activity of Different Self-etching Primers and Adhesives. J Contemp Dent Pract 2008; 9 (7):57-64.
The aim of this study was to evaluate the antibacterial effects of different one-step and two-step selfetching primer/adhesives on Streptococcus mutans (S. mutans), Lactobacillus casei (L. casei), and Lactobacillus acidophilus (L. acidophilus).
Methods and Materials
The antibacterial effects of Clearfil Protect Bond Primer and Bonding agent; AdheSE Primer and Bonding agent; Adper Prompt L-Pop; Futurabond NR; Clearfil Tri S Bond; and Cervitec (positive control, 1% chlorhexidine varnish) were tested against standard strains of S. mutans, L. Casei, and L. acidophilus using the disk diffusion method. Standard filter paper disks (n=5) impregnated with 20 microL of each material were prepared. After incubation at 37°C for 48 hours in a 5-10% CO2 atmosphere, the diameter of inhibition zones were measured in millimeters. Data were analyzed using one way analysis of variance (ANOVA) and multivariate analysis of variance (MANOVA). Duncan's Multiple Range Test was used for pairwise comparison.
Results
The size of inhibition zones produced by primer/adhesives varied among the brands. AdheSE Primer: S. mutans (20.6±1.51); L. casei (14.8±1.78); L. acidophilus (11.4±0.54). Adper Prompt L-Pop: S. mutans (19.6±1.51); L. casei (13.8±1.64); L. acidophilus (13.8±1.09). Cervitec: S. mutans (23±0.00); L. casei (27±0.70); L. acidophilus (22.4±0.54). Clearfil Protect Bond Primer: S. mutans (17±0.00); L. casei (17.6±0.54); L. acidophilus (22.4±0.54). Futurabond NR was found effective only against S. mutans (14.6±1.67). Of all the materials tested, AdheSE Bonding agent, Clearfil Protect Bond Bonding agent, and Clearfil Tri S Bond exhibited no inhibition zone (-) for all bacteria tested.
Conclusion
Among the adhesives tested Clearafil Protect Bond Primer based upon monomer methacryloyloxydodecylpyridiniium bromide (MDPB) was found to be the most potent material against L. acidophilus and L. casei. AdheSE Primer and Adper Prompt L-Pop are highly effective against S. mutans.
Clinical Significance
Compared with other adhesive systems, Clearfil Protect Bond Primer (containing MDPB) showed a high antibacterial effect against all microorganizms tested. Two-step, self-etching primer/ adhesive system Clearfil Protect Bond might be a suitable choice under minimally invasive restorations. The recently developed one-step, self-etching system Clearfil Tri S Bond showed no antibacterial effect against microorgazims tested.
Citation
Korkmaz Y, Ozalp M, Attar N. Comparison of the Antibacterial Activity of Different Self-etching Primers and Adhesives. J Contemp Dent Pract 2008 November; (9)7:057-064.
Effect of multi-step dentin bonding systems and resin-modified glass ionomer cement liner on marginal quality of dentin-bonded resin composite Class II restorations. Clin Oral Investig 1998; 2:130-136.
Microbial microleakage and pulpal inflammation: a review. Endod Dent Traumatol 1986; 2:177-183.
Frequency of secondary caries at various anatomical locations. Oper Dent 1985; 10:88-92.
Histobacteriological analysis of acid red dye-stainable dentin found beneath intact amalgam restorations. Oper Dent 1994; 19:65-69.
An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact dentin. J Adhes Dent 2000; 2:83-98.
Influence of additional acid etch treatment on resin cement dentin infiltration. J Prosthodont 2000; 9:77-81.
Practitioner variability effects on dentin bonding with an acetone-based one-bottle adhesive. J Adhes Dent 1999; 1:311-314.
“No-bottle” vs “multi-bottle” dentin adhesives-a microtensile bond strength and morphological study. Dent Mater 2001; 17:373-380.
Bonding to root caries by a self-etching adhesive system containing MDPB. Am J Dent. 2004; 17:89-93.
Antibacterial activity of proprietary self-etching primers. Am J Dent 1998; 11:106-108.
Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 2003; 19:449-457.
Comparison of antibacterial activity of simplified adhesive systems. Am J Dent 2002; 5:356-360.
(formerly NCCLS) Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, Ninth Ed.,M2-A9, 2006 Villanova PA.
Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent 2003; 28:215-235.
Clinical performance of self-etch adhesives at 18 months, Am J Dent 2005; 18: 2135-2140.
The reason for replacement and the age of failed restorations in general dental practice. Acta Odontol Scand. 1997; 55:58-63.
In vivo antibacterial effects of dentin primer incorporating MDPB. Oper Dent 2004; 29(4):369-375.
Mutans streptococci in plaque from margins of amalgam, composite, and glass-ionomer restorations. J Dent Res 1990; 69:861-864.
Clinical and oral microbiology. Washington Hemisphere Publishing, 1998; 3:474-478.
Bacterial penetration, pulpal reaction and the inner surface of Concise enamel bond. Composite fillings in etched and unetched cavities. J Dent Res 1978; 57:3-10.
Growth and acid tolerance of human dental plaque bacteria. Arch Oral Biol 1984; 29:843-848.
Effect of dentin on the antibacterial activity of dentin bonding agents. J Endod 2004; 30:352-358.
Susceptibility of various microorganizms to chlorhexidine. Scand J Dent Res 1977; 85:255-265.
Antibacterial activity of two adhesive systems using various microbiological methods. J Adhes Dent 2005; 7:315-320.
Antimicrobial properties of self-etching primer and bonding systems. Oper Dent 2003;28:143-148.