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VOLUME 13 , ISSUE 3 ( May-June, 2012 ) > List of Articles


Blood Cells Attachment after Root Conditioning and PRP Application: An in vitro Study

Fabio Renato Manzolli Leite, José Eduardo Cezar Sampaio, Lucas Amaral Fontanari, Eduardo de Paula Ishi, Daniela Leal Zandim, Andrea Abi Rached Dantas, Ricardo Samih Georges Abi Rached

Citation Information : Leite FR, Sampaio JE, Fontanari LA, de Paula Ishi E, Zandim DL, Dantas AA, Rached RS. Blood Cells Attachment after Root Conditioning and PRP Application: An in vitro Study. J Contemp Dent Pract 2012; 13 (3):332-338.

DOI: 10.5005/jp-journals-10024-1147

License: CC BY-NC 3.0

Published Online: 01-06-2012

Copyright Statement:  Copyright © 2012; Jaypee Brothers Medical Publishers (P) Ltd.



Root conditioning is aimed at smear layer removal and at dental matrix collagen exposure, which may promote periodontal regeneration. This in vitro study assessed smear layer removal, collagen fiber exposure and the influence of PRP (platelet-rich plasma) application on adhesion of blood cells to the root surface using scanning electron microscopy (SEM).

Materials and methods

Scaled root samples (n = 160) were set in five groups and conditioned with: group I – control group (saline solution); group II (EDTA 24%); group III (citric acid 25%); group IV (tetracycline hydrochloride 50 mg/ml); group V (sodium citrate 30%). Eighty samples were assessed using the root surface modification index (RSMI). The other eighty samples were set in two groups. The first group (n = 40) received PRP gel application with a soft brush and the second group (n = 40) received PRP application and then a blood drop. The fibrin clot formation was assessed in the first group and the blood cells adhesion was assessed in the second group using the BEAI (blood elements adhesion index). A previously trained, calibrated, and blind examiner evaluated photomicrographs. Statistical analysis was performed using the Kruskal-Wallis's and Dunn's tests.


Group III attained the best results for RSMI and BEAI. Moreover, it was the only group showing fibrin clot formation.


Citric acid was the most efficient conditioner for smear layer removal, collagen fiber exposure and blood cell adhesion. Moreover, it was the only group showing fibrin clot formation after PRP application.

Clinical significance

This study demonstrated that root conditioning followed by PRP application may favor blood cell adhesion on root surface which may optimize periodontal healing.

How to cite this article

Dantas AAR, Fontanari LA, Ishi EP, Leite FRM, Zandim DL, Abi Rached RSG, Sampaio JEC. Blood Cells Attachment after Root Conditioning and PRP Application: An in vitro Study. J Contemp Dent Pract 2012;13(3):332-338.

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