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VOLUME 15 , ISSUE 2 ( March-April, 2014 ) > List of Articles


In situ Assessment of Effects of the Bromide- and Fluoride-incorporating Adhesive Systems on Biofilm and Secondary Caries

Suyane Maria Luna Cruz de Vasconcelos, Mary Anne Sampaio de Melo, Joao Paulo Marques Saraiva Wenceslau, Iriana Carla Junqueira Zanin, Haroldo Cesar Pinheiro Beltrao, Carlos Augusto Oliveira Fernandes, Paulo Cesar de Almeida, Lidiany Karla Azevedo Rodrigues

Citation Information : de Vasconcelos SM, de Melo MA, Wenceslau JP, Zanin IC, Beltrao HC, Fernandes CA, de Almeida PC, Rodrigues LK. In situ Assessment of Effects of the Bromide- and Fluoride-incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014; 15 (2):142-148.

DOI: 10.5005/jp-journals-10024-1504

Published Online: 01-10-2014

Copyright Statement:  Copyright © 2014; The Author(s).



This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization.

Materials and methods

During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SETM (self-etch, fluoride/MDPB free adhesive, AB) and Clearfil Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S (ƒ¢S) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 ƒÊm from the restoration margin. Data were analyzed by ANOVA.


No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm.


All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research.

How to cite this article

de Vasconcelos SMLC, de Melo MAS, Wenceslau JPMS, Zanin ICJ, Beltrao HCP, Fernandes CAO, de Almeida PC, Rodrigues LKA. In situ Assessment of Effects of the Bromide- and Fluoride-Incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014;15(2):142-148.

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  1. Microtensile bond strengths of one-and two-step self-etch adhesives to bur-cut enamel and dentin. Am J Dent 2003;16:414-420.
  2. Clinical diagnosis of recurrent caries. J Am Dent Assoc 2005;136:1426-1433.
  3. Immediate performance of selfetch versus system adhesives with multiple light-activated restoratives. Dent Mater 2004;20:873-880.
  4. self-etch dentin primers containing Phenyl-P. J Dent Res 1994;73:1088-1095.
  5. Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 2003;19:449-457.
  6. In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. Dent Mater 2006;22:527-532.
  7. Incorporation of antibacterial monomer MDPB into dentin primer. J Dent Res 1997;76:768-772.
  8. Antibacterial activity of cured dental resin incorporating the antibacterial monomer MDPB and an adhesion-promoting monomer. J Biomed Mater Res 1998;39:511-515.
  9. Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater 2003;19:313-319.
  10. Incorporation of bacterial inhibitor into resin composite. J Dent Res 1994;73:1437-1443.
  11. Antibacterial activities of MDPB and fluoride in dentin bonding agents. Eur J Prosthodont Dent 2005;13:139-142.
  12. Antibacterial activity of dentine primer containing MDPB after curing. J Dent 1998;26:267-271.
  13. In situ anticariogenic potential of glass ionomer cement. Caries Res 1993;27:280-284.
  14. Effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms. Caries Res 2002;36:81-86.
  15. Active root surface caries converted into inactive caries as a response to oral hygiene. Scand J Dent Res 1986;94:281-284.
  16. Inhibition of artificial secondary caries by fluoride-releasing adhesives on root dentin. J Oral Rehabil 2002;29:523-527.
  17. Antibacterial activity of proprietary self-etch primers. Am J Dent 1998;11:106-108.
  18. Bactericidal effect of dentin primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB) against bacteria in human carious dentin. J Oral Rehabil 2001;28:314-319.
  19. Influence of human dentine on the antibacterial activity of self-etch adhesive systems against cariogenic bacteria. J Dent 2008;36:241-248.
  20. In situ mineral loss inhibition by CO2 laser and fluoride. J Dent Res 2006;85:617-621.
  21. Validity issues in split-mouth trials. J Clin Periodontol 1992;19:625-627.
  22. Effect of Plaque Fluoride Released from a Glass-Ionomer Cement on Enamel Remineralization in situ. Caries Res 2005;39:157-160.
  23. Caries progression and inhibition in human and bovine root dentine in situ. Caries Res 2003;37:339-344.
  24. Biochemical composition and cariogenicity of dental plaq ue formed in the presence of sucrose or glucose and fructose. Caries Res 2000;34:491-497.
  25. Correlation of transversal microradiography and microhardness on in situ-induced demineralization in irradiated and non-irradiated human dental enamel. Arch Oral Biol 1999;44:243-248.
  26. The short-term in situ model to evaluate the anticariogenic potential of ionomeric materials. J Dent 2005;33:491-497.
  27. Effect of Sucrose Containing Iron (II) on Dental Biofilm and Enamel Demineralization in situ. Caries Res 2005;39:123-129.
  28. The importance of fluoride dentifrices to the current dental caries prevalence in Brazil. Braz Dent J 2004;15:167-174.
  29. Dentifrices, mouthwashes, and remineralization/caries arrestment strategies. BMC Oral Health 2006;6:1-13.
  30. History of water fluoridation. Br Dent J 2005;8:1-4.
  31. Enamel demineralization in situ with various frequencies of carbohydrate consumption with and without fluoride toothpaste. J Dent Res 2001;80:1721-1724.
  32. In vitro evaluation of caries inhibition promoted by self-etch adhesive systems containing antibacterial agents. J Biomed Res B Appl Biomater 2005;75:122-127.
  33. Fluoride-induced enhancement of diffusion in streptococcal model plaque biofilms. Caries Res 1998;32:227-232.
  34. In vitro biofilm formation on the surface of resin-based dentine adhesive. Eur J Oral Sci 2006;114:243-249.
  35. Adhesive systems and secondary caries formation: Assessment of dentin bond strength, caries lesions depth and fluoride release. Dent Mater 2007;23:308-316.
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