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VOLUME 16 , ISSUE 9 ( September, 2015 ) > List of Articles

RESEARCH ARTICLE

Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens

Zoubeida AL Yahfoufi, Wahib Hadchiti, Antoine Berberi

Citation Information : Yahfoufi ZA, Hadchiti W, Berberi A. Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens. J Contemp Dent Pract 2015; 16 (9):727-732.

DOI: 10.5005/jp-journals-10024-1748

Published Online: 01-09-2015

Copyright Statement:  Copyright © 2015; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Background

In clinical microbiology several techniques have been used to identify bacteria. Recently, Deoxyribonucleic acid (DNA)-based techniques have been introduced to detect human microbial pathogens in periodontal diseases. Deoxyribonucleic acid probes can detect bacteria at a very low level if we compared with the culture methods. These probes have shown rapid and cost-effective microbial diagnosis, good sensitivity and specificity for some periodontal pathogens in cases of severe periodontitis.

Materials and methods

Eighty-five patients were recruited for the study. Twenty-one subjects ranging between 22 and 48 years of age fulfilled the inclusion and exclusion criteria. Seventy-eight samples became available for DNA probe analysis from the deepest pockets in each quadrant.

Results

All 21 patients showed positive results for Prevotella intermedia; also, Prevotella gingivalis was identified in 19 subjects, Aggregatibacter actinomycetemcomitans in 6 subjects. P. intermedia was diagnosed positive in 82% of the subgingival samples taken, 79% for P. gingivalis, and 23% for A. actinomycetemcomitans.

Conclusion

This study shows a high frequency of putative periodontal pathogens by using DNA probe technology, which is semi-quantitative in this study. Deoxyribonucleic acid probes can detect bacteria at very low level about 103 which is below the detection level of culture methods. The detection threshold of cultural methods.

Clinical significance

The three types of bacteria can be detected rapidly with high sensitivity by using the DNA probe by general practitioners, and thus can help in the diagnosis process and the treatment.

How to cite this article

Yahfoufi ZAL, Hadchiti W, Berberi A. Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens. J Contemp Dent Pract 2015;16(9): 727-732.


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