The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 17 , ISSUE 11 ( November, 2016 ) > List of Articles

RESEARCH ARTICLE

Assessment of Myeloperoxidase and Nitric Levels around Dental Implants and Natural Teeth as a Marker of Inflammation: A Comparative Study

Gayithri H Kulkarni, Prashant Jadhav, Kiran Kulkarni, Sachin V Shinde, Yojana B Patil

Citation Information : Kulkarni GH, Jadhav P, Kulkarni K, Shinde SV, Patil YB. Assessment of Myeloperoxidase and Nitric Levels around Dental Implants and Natural Teeth as a Marker of Inflammation: A Comparative Study. J Contemp Dent Pract 2016; 17 (11):934-938.

DOI: 10.5005/jp-journals-10024-1957

Published Online: 01-11-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction

Dental implants form the mainstay of dental treatment involving rehabilitation of missing teeth. One of the major concerns for the clinicians doing dental implants is the postsurgical failure of dental implants. Success of dental implants is dependent upon the skills of the surgeon and the amount and quality of the bone remaining at the edentulous area where dental implant has to be placed. Myeloperoxidase (MPO) and nitrites are few of the enzymes and molecules which are said to be altered in inflammation. However, their exact role in the inflammatory processes around natural tooth and dental implant is still unclear. Hence we comparatively evaluated the levels of MPO and nitrites in the areas around the dental implants and natural teeth.

Materials and methods

The present study comprises 42 patients who underwent prosthetic rehabilitation by dental implants from 2011 to 2014. Depth of probing value (DP), score of plaque index (SPI), gingival index (GI), and index of gingival bleeding time (GBT) were evaluated for the assessment of the periimplant soft tissue changes. Assessment of inflammation around the dental implant surface and around natural tooth was done based on the readings of these parameters. For the measurement of the MPO levels, spectrophotometric MPO assay was used. All the results were analyzed by Statistical Package for the Social Sciences (SPSS) software.

Results

The mean plaque index values were 1.56 and 0.97 in periodontitis cases of natural teeth and inflamed cases of dental implants respectively. While comparing mean plaque index, mean probing depth, and mean gingival bleeding index in between the two groups, significant difference was obtained. Mean MPO concentration in periodontitis and gingivitis cases in natural teeth were 0.683 and 0.875 U/μL, while in inflamed dental implant cases, the mean value was 0.622 U/μL. While comparing the total MPO levels, total nitrite levels, and total nitrite concentration in between two study groups, significant difference was obtained. On comparing the healthy and periodontitis cases in natural teeth, significant difference was obtained.

Conclusion

In the inflammatory processes occurring around dental implant and natural teeth, MPO and NO make some amount of significant contribution.

Clinical significance

The present study enforces on the role of MPO and nitrite as diagnostic and prognostic marker.

How to cite this article

Kulkarni GH, Jadhav P, Kulkarni K, Shinde SV, Patil YB, Kumar M. Assessment of Myeloperoxidase and Nitric Levels around Dental Implants and Natural Teeth as a Marker of Inflammation: A Comparative Study. J Contemp Dent Pract 2016;17(11):934-938.


PDF Share
  1. Dental implants in periodontal therapy. J Periodontol 2000 Dec;71(12):1934-1942.
  2. Analysis of gingival crevicular fluid intracytoplasmic enzyme activity in patients with adult periodontitis and rapidly progressive periodontitis. A longitudinal study model with periodontal treatment. J Periodontol 1998 Oct;69(10):1155-1163.
  3. Peri-implantitis. Dent Clin North Am 2005 Jul;49(3):661-676.
  4. Treatment of periimplant infections: a literature review. J Clin Periodontol 2003 Jun;30(6):467-485.
  5. Myeloperoxidase level around dental implants as an indicator of an inflammatory process. Indian J Dent 2015 Jan-Mar;6(1):2-6.
  6. Dimensions and relations of dentogingival junction in humans. J Periodontol 1961 Jul;32(3):261-267.
  7. Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 1964 Feb;22:121-135.
  8. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand 1963 Dec;21: 533-551.
  9. The gingival bleeding time index. J Periodontol 1981 May;52(5):260-262.
  10. Measurement of nitrate and nitrite in extracellular fluids: a window to systemic nitric oxide metabolism. Methods 1995 Feb;7(1):84-89.
  11. Assay method for myeloperoxidase in human polymorphonuclear leukocytes. Anal Biochem 1983 Jul;132(2):345-352.
  12. Ligature-induced periimplant infection in cynomolgus monkeys. I. Clinical and radiographic findings. Clin Oral Implants Res 1993 Mar;4(1):2-11.
  13. Experimental breakdown of periimplant and periodontal tissues. A study in the beagle dog. Clin Oral Implants Res 1992 Mar;3(1):9-16.
  14. Ligature-induced marginal inflammation around osseointegrated implants and ankylosed teeth: Stereologic and histologic observations in cynomolgus monkeys (Macaca fascicularis). J Periodontol 1993 Jun;64(6):529-537.
  15. Ligature-induced marginal inflammation around osseointegrated implants and ankylosed teeth. Clinical and radiographic observations in cynomolgus monkeys (Macaca fascicularis). Clin Oral Implants Res 1993 Mar;4(1):12-22.
  16. Nature 1998 Jan;391(6665):393-397.
  17. Crevicular fluid myeloperoxidase at healthy, gingivitis and periodontitis sites. J Clin Periodontol 1989 Jan;16(1):17-20.
  18. Myeloperoxidase and cardiovascular disease. Arterioscler Thromb Vasc Biol 2005 Jun;25(6):1102-1111.
  19. The importance of data presentation regarding gingival crevicular fluid myeloperoxidase and elastase-like activity in periodontal disease and health status. J Periodontol 2000 Mar;71(3):460-467.
  20. Crevicular fluid enzymes from endosseous dental implants and natural teeth. Int J Oral Maxillofac Implants 1996 May-Jun;11(3):322-330.
  21. A review on peri-implant crevicular fluid assays potential in monitoring and predicting periimplant tissue responses. J Int Acad Periodontol 2002 Apr;4(2):49-59.
  22. Correlation of peri-implant health and aspartate aminotransferase levels: a cross-sectional clinical study. Int J Oral Maxillofac Implants 2000 Jul-Aug;15(4):500-504.
  23. Correlation of peri-implant health and myeloperoxidase levels: a cross-sectional clinical study. Clin Oral Implants Res 2004 Oct;15(5):546-552.
  24. Analysis of the possible impact of inflammation severity and early and delayed loading on nitric oxide metabolism around dental implants. Int J Oral Maxillofac Implants 2005 Jul-Aug;20(4):547-556.
  25. Cathepsin B/L-, elastase-, tryptase-, trypsin and dipeptidyl peptidase IV-like activities in gingival crevicular fluid: Correlation with clinical parameters in untreated chronic periodontitis patients. J Periodontal Res 1992 Jan;27(1):62-69.
  26. Crevicular fluid level of beta-glucuronidase in relation to clinical periodontal parameters and putative periodontal pathogens in early-onset periodontitis. J Clin Periodontol 1998 Aug;25(8):630-639.
  27. Analysis of the inflammatory process around endosseous dental implants and natural teeth: Myeloperoxidase level and nitric oxide metabolism. Int J Oral Maxillofac Implants 2007 Nov-Dec;22(6):969-979.
  28. Myeloperoxidase as a measure of polymorphonuclear leukocyte response in inflammatory status around immediately and delayed loaded dental implants: a randomized controlled clinical trial. Clin Implant Dent Relat Res 2008 Mar;10(1):30-39.
  29. Analysis of cathepsin-K levels in biologic fluids from healthy or diseased natural teeth and dental implants. Int J Oral Maxillofac Implants 2011 Sep-Oct;26(5):991-997.
  30. Analysis of cathepsin-K activity at tooth and dental implant sites and the potential of this enzyme in reflecting alveolar bone loss. J Periodontol 2012 Apr;83(4):498-505.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.