Evaluation of Inflammatory Response to Endodontic Sealers in a Bone Defect Animal Model
Miriam Z Scelza, Carlos AM Campos, Pantaleo Scelza, Caroline SR Adeodato, Igor B Barbosa, Fernando de Noronha, Victor Montalli, Marcelo Napimoga, Vera C de Araújo,1, Gutemberg G Alves
Citation Information :
Scelza MZ, Campos CA, Scelza P, Adeodato CS, Barbosa IB, de Noronha F, Montalli V, Napimoga M, de Araújo,1 VC, Alves GG. Evaluation of Inflammatory Response to Endodontic Sealers in a Bone Defect Animal Model. J Contemp Dent Pract 2016; 17 (7):536-541.
The aim of this study was to evaluate the inflammatory response to MTA Fillapex, AH Plus, and Pulp Canal Sealer Extensive Work Time (EWT), in a murine bone defect grafting model.
Materials and methods
Bilateral mandibular critical defects were produced in 45 Wistar rats with a trephine bur#2 and filled with the endodontic sealers. After 7, 14, and 28 days, the rats were euthanized and their jaws were histologically prepared.
Results
For the 7-day group, no statistical significance was observed among all studied groups (p > 0.05), and high levels of inflammatory infiltrate were detected. After 14 and 28 days, Pulp Canal Sealer EWT showed statistically lower inflammatory response in comparison to other sealers (p < 0.05) except for the control group (no sealers).
Conclusion
Pulp Canal Sealer EWT presented the lowest levels of inflammatory response. The critical defect grafting model was an effective method to detect differences among differences on the biological response to endodontic sealers.
Clinical significance
Knowing the biocompatibility of endodontics sealers that will be used in filling the root canal.
How to cite this article
Scelza MZ, Campos CAM, Scelza P, Adeodato CSR, Barbosa IB, de Noronha F, Montalli V, Napimoga M, de Araújo VC, Alves GG. Evaluation of Inflammatory Response to Endodontic Sealers in a Bone Defect Animal Model. J Contemp Dent Pract 2016;17(7):536-541.
Biocompatibility of an experimental glass-ionomer cement sealer in rat mandibular bone. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003 Oct;96(4):458-465.
Cytotoxicity evaluation of various resin based root canal sealers. Int Endod J 2010 Feb;43(2):148-153.
Materials used for root canal obturation: technical, biological and clinical testing. Endod Topics 2005 Nov;12(1):25-38.
ASTM Standard Test Method F 0813-07 ed. 2007.
Dentistry – Preclinical evaluation of biocompatibility of medical devices used in dentistry – Test methods for dental materials. ISO 7405:. ISO 7405: 2008;2008.
Biological evaluation of medical devices – Part 1: evaluation and testing within a risk management process. ISO 10993-1 ed.; 2009.
Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod 2009 Jul;35(7):1051-1055.
A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts. Int Endod J 2012 Jan;45(1):12-18.
In vitro osteogenic potential of an experimental calcium silicate-based root canal sealer. J Endod 2010 Jul;36(7):1163-1169.
Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J 2005 Sep;38(9):604-609.
Long-term radiographic and histological observations of endodontically treated teeth. J Endod 1999 Dec;25(12):818-822.
Biological evaluation of medical devices – Part 6: tests for local effects after implantation. ISO 10993-6 ed.; 2007.
Evaluation of inflammatory response of EDTA, EDTA-T, and citric acid in animal model. J Endod 2010 Mar;36(3):515-519.
The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res 1986 Apr;(205):299-308.
Study of growth factors and receptors in carcinoma ex pleomorphic adenoma. J Oral Pathol Med 2010 Aug;39(7):540-547.
Increased mucin 1 expression in recurrence and malignant transformation of salivary gland pleomorphic adenoma. Histopathology 2011 Feb;58(3):377-382.
In vitro evaluation of macrophage viability after incubation in orange oil, eucalyptol, and chloroform. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006 Sep;102(3):e24-e27.
Cytotoxicity of endodontic materials over 6-weeks ex vivo. Int Endod J 2008 Dec;41(12):1072-1078.
Stability of silorane dental monomers in aqueous systems. J Dent 2006 Jul;34(6):405-410.
Biological aspects of root canal filling materials – histocompatibility, cytotoxicity, and mutagenicity. Clin Oral Investig 1997 Mar;1(1):5-11.
Release of formaldehyde by 4 endodontic sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999 Aug;88(2):221-225.
Comparison of the intraosseous biocompatibility of AH Plus, EndoREZ, and Epiphany root canal sealers. J Endod 2006 Jul;32(7):656-662.
Cytotoxicity and genotoxicity of root canal sealers based on mineral trioxide aggregate. J Endod 2012 Apr;38(4):495-500.
Cytocompatibility of the ready-to-use bioceramic putty repair cement iRoot BP Plus with primary human osteoblasts. Int Endod J 2012 Jun;45(6):508-513.
Mineral trioxide aggregate-based sealer: analysis of tissue reactions to a new endodontic material. J Endod 2010 Jul;36(7):1174-1178.
Calcium salicylate-mediated apoptosis in human HT-1080 fibrosarcoma cells. Cell Prolif 2006 Aug;39(4):249-260.
Toxicity of endodontic filling materials. Endod Topics 2005 Nov;12(1):39-43.
