The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 18 , ISSUE 4 ( April, 2017 ) > List of Articles


Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation

Carlos Estrela, Rafaella M Chaves, Paula C Cardoso, Terezinha de JE Barata, João B de Souza, Érica M de Torres, Cyntia RA Estrela, Ana PR Magalhães, Lawrence G Lopes

Citation Information : Estrela C, Chaves RM, Cardoso PC, de JE Barata T, de Souza JB, de Torres ÉM, Estrela CR, Magalhães AP, Lopes LG. Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation. J Contemp Dent Pract 2017; 18 (4):265-269.

DOI: 10.5005/jp-journals-10024-2029

Published Online: 01-08-2017

Copyright Statement:  Copyright © 2017; The Author(s).



This study aims to assess the effect of ozone gas on dentin exposed to Streptococcus mutans biofilm by evaluation of mineral content [log calcium-to-phosphorus (Ca/P)] using energy-dispersive X-ray (EDX) spectroscopy.

Materials and methods

Five human third molars were sectioned into four slices of dentin and distributed in four groups: I – control (no treatment); II – ozone therapy; III – biofilm development; IV – ozone therapy followed by biofilm development. Mineral content (log Ca/P) was evaluated by EDX. Data were analyzed by analysis of variance and Tukey's test (p < 0.05).


Results showed that the mineral content of control group (I) was similar to ozone group (II), and was statistically higher than biofilm (III) and ozone + biofilm (IV). The lowest log Ca/P was determined in biofilm group (III).


It can be concluded that ozone gas did not grant preventive effects of demineralization by S. mutans biofilm on dentin surface.

Clinical significance

Ozone gas therapy may be an alternative noninvasive treatment aiming to reduce the levels of caries-associated microorganisms. This therapy may, thereby, be an alternative and/or complementary treatment strategy in preventive dentistry.

How to cite this article

Chaves RM, Estrela C, Cardoso PC, de JE Barata T, de Souza JB, de Torres EM, Estrela CRA, Magalhães APR, Lopes LG. Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation. J Contemp Dent Pract 2017;18(4):265-269.

PDF Share
  1. Antibacterial effect of ozone on cariogenic bacterial species. J Dent 2009 Jun;37(6):449-453.
  2. Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. Eur J Oral Sci 2007 Feb;115(1):77-80.
  3. ; Lynch, E.; Grootveld, M. The use of ozone for the management of primary root carious lesions. In: Albrektsson, TO.; Bratthall, D.; Glantz, PI.; Lindhe, JT., editors. Tissue preservation and caries treatment. Surrey: Quintessence Publishing Company; 2001. p. 49-67.
  4. Effect of ozone treatment on different cariogenic microorganisms in vitro. Swed Dent J 2008;32(3):139-147.
  5. The inability of Streptococcus mutans and Lactobacillus acidophilus to form a biofilm in vitro on dentine pretreated with ozone. Aust Dent J 2008 Dec;53(4):349-353.
  6. The impact of ozone treatment on enamel physical properties. Am J Dent 2006 Feb;19(1):67-72.
  7. Protection offered by root-surface restorative materials against biofilm challenge. J Dent Res 2007 May;86(5):431-435.
  8. The application of ozone in dentistry: a systematic review of literature. J Dent 2008 Feb;36(2):104-116.
  9. Assessment of the ozone-mediated killing of bacteria in infected dentine associated with non-cavitated occlusal carious lesions. Caries Res 2007;41(5):337-341.
  10. Clinical reversal of root caries using ozone: 6-month results. Am J Dent 2007 Aug;20(4):203-208.
  11. Antimicrobial efficacy of ozonated water, gaseous ozone, sodium hypochlorite and chlorhexidine in infected human root canals. Int Endod J 2007 Feb;40(2):85-93.
  12. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endod J 2005 Jan;38(1):22-29.
  13. Using ozone to treat dental caries. J Esthet Restor Dent 2007;19(6):303-305.
  14. Synergistic antimicrobial action of chlorhexidine and ozone in endodontic treatment. Biomed Res Int 2014;2014:592423.
  15. Mitochondrial reticulum network dynamics in relation to oxidative stress, redox regulation, and hypoxia. Int J Biochem Cell Biol 2009 Oct;41(10):1790-1804.
  16. Comparison of the immediate effects of gaseous ozone and chlorhexidine gel on bacteria in cavitated carious lesions in children in vivo. Clin Oral Investig 2009 Sep;13(3):287-291.
  17. Clinical reversal of root caries using ozone, double-blind, randomised, controlled 18-month trial. Gerodontology 2003 Dec;20(2):106-114.
  18. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci 2006 Aug;114(4):349-353.
  19. Ozone as Janus: this controversial gas can be either toxic or medically useful. Mediators Inflamm 2004 Feb;13(1):3-11.
  20. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006 May;37(4):425-435.
  21. Ozone therapy in medicine and dentistry. J Contemp Dent Pract 2008 May;9(4):75-84.
  22. The use of ozone in dentistry and maxillofacial surgery: a review. Quintessence Int 2006 May;37(5):353-359.
  23. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 2004 Nov;30(11):778-781.
  24. DNA degradation with ozone. Int J Biol Macromol 2006 May;38(3-5):248-254.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.