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VOLUME 19 , ISSUE 10 ( 2018 ) > List of Articles

ORIGINAL RESEARCH

Establishment of Role of IL-2, IL-10 and IL-12 in Patients with Recurrent Aphthous Stomatitis—A Clinical Study

Satish S Bhosale, Bhoopendra S Rajput, Harsh Takkar, Sachin V Bhagat, Rajesh M Vagger, Mohd. Ibrahim K Shaikh

Keywords : Interleukins, Recurrent aphthous stomatitis, Stress

Citation Information : Bhosale SS, Rajput BS, Takkar H, Bhagat SV, Vagger RM, Shaikh MI. Establishment of Role of IL-2, IL-10 and IL-12 in Patients with Recurrent Aphthous Stomatitis—A Clinical Study. J Contemp Dent Pract 2018; 19 (10):1242-1245.

DOI: 10.5005/jp-journals-10024-2411

License: CC BY-NC 3.0

Published Online: 00-10-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Background: Recurrent aphthous stomatitis is a vesiculobullous disease characterized by painful ulcers in the oral cavity. The role of interleukins such as IL-2, IL-10 and IL-12 in initiating disease demands careful assessment. The present study was conducted to determine the level of IL-2, IL-10 and IL-12 in patients with recurrent aphthous stomatitis. Materials and methods: The present study was conducted on 40 patients diagnosed with recurrent aphthous stomatitis. An equal number of age and gender-matched subjects (40) was included as a control. They were divided into 2 age groups from 20 to 40 years and 40 to 60 years. All were made to sit comfortably on a dental chair, and 1 ml of unstimulated saliva was collected in a sterile tube to assess the level of IL-2, IL-10 and IL-12 using ELISA. The level of IL-2, IL-10 and IL-12 was measured in pg/mL. Results: Each group had 10 males and 10 females. The difference was non-significant (P-1). Age group 20-40 years comprised of 14 patients in group I (eight males and six females) and 12 in group II (five males and seven females). Age group 40-60 years had six patients in group I (two males and four females) and 8 patients in group II (five males and three females). The difference was significant (p < 0.05). The most common form was minor (82%) followed by herpetiform (13%) and major (5%). In group I, the mean value of IL-2 level was 32.24 pg/mL, IL-10 was 1.24± 0.6 and IL-12 was 28.34± 4.04 and in group II, mean value of IL-2 level was 12.10 pg/mL, IL-10 was 2.56± 1.12 and IL-12 was 23.16± 4.16. The difference was significant (p < 0.05). Conclusion: Age group 20 to 40 years showed higher prevalence. The level of IL-2 and IL-12 is highly increased while IL-10 is decreased in patients. Clinical significance: Role of interleukins as a precipitating factor along with stress is well established. With the thorough knowledge of the disease process, the newer treatment modality specific against interleukins may be proven useful in controlling the disease.


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  1. Rogers RS. Recurrent aphthous stomatitis in the diagnosis of Behcet's disease. Yonsei Med J 1997;38:370-379.
  2. Scully C, Gorsky M, Lozada Nur F. The diagnosis and management of recurrent aphthous stomatitis: A consensus approach. J Am Dent Assoc 2003; 134:200-207.
  3. Porter SR, Hegarty A, Kaliakatsou F, Hodgson TA, Scully C. Recurrent aphthous stomatitis. Clin Dermatol 2000; 18:569-578.
  4. Jurge S, Kuffer R, Scully C, Porter SR. Mucosal disease series. Number VI. Recurrent aphthous stomatitis. Oral Dis 2006;12:1-21.
  5. Preethi L, Magesh K, Rajkumar K, Karthik R. Recurrent aphthous stomatitis. J Oral Maxillofac Pathol 2011;15:252- 256.
  6. Nowak M, Gorska R. Comparison of IL-2 concentration in peripheral blood and stimulated saliva found in individuals suffering from recurrent aphthous stomatitis (RAS) and in healthy ones. Czas Stomatol 2008;61:387-394.
  7. Amador-Pattaroyo MJ, Rodriguez-Rodriguez A, Montoya Ortiz G How does age at onset influence the outcome of autoimmune diseases. Autoimmune Dis 2012;2:517-530.
  8. Gorska R. Epidemiologic studies of oral mucosa changes occurring in children, adolescents, and adults 13–24 years of age in Warsaw. Przegl Epidemiol 1997;51:339-347.
  9. Albanidou-Farmaki E, Markopoulos AK, Kalogerakou F et al. Detection, enumeration and characterization of T helper cells secreting type 1 and type 2 cytokines in patients with recurrent aphthous stomatitis. Tohoku J Exp Med 2007; 212:101-105.
  10. Buno IJ, Huff C, Weston WL et al. Elevated levels of interferon gamma, tumor necrosis factor a, interleukins 2, 4, and 5, but not interleukin 10, are present in recurrent aphthous stomatitis. Arch Dermatol 1998;134:827-831.
  11. Miyamoto NT Jr, Borra RC, Abreu M et al. Immune expression of HSP27 and IL-10 in recurrent aphthous ulceration. J Oral Pathol Med 2008;37:462-467.
  12. Lewkowicz N, Banasik M, Tchorzewski H et al. Predominance of production of Th1 type cytokines in recurrent aphthous ulceration. Dent Med Probl 2004;42:655-660.
  13. Guimaraes AL, Correia-Silva Jde F et al. Investigation of functional gene polymorphisms IL-1b, IL-6, IL-10 and TNFalpha in individuals with recurrent aphthous stomatitis. Arch Oral Biol 2007;52:268-272.
  14. Huling LB, Baccaglini L, Choquette L et al. Effect of stressful life events on the onset and duration of recurrent aphthous stomatitis. J Oral Pathol Med 2012;41:149-152.
  15. Keenan AV, Spivakovksy S Stress associated with onset of recurrent aphthous stomatitis. Evid Based Dent 2013; 14:25-27.
  16. Natah SS, Hayrinen-Immonen R, Hietanen J et al. Immunolocalization of tumor necrosis factor-alpha expressing cells in recurrent aphthous ulcer lesions (RAU). J Oral Pathol Med 2000;29:19-25.
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