The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 19 , ISSUE 12 ( December, 2018 ) > List of Articles

ORIGINAL ARTICLE

An In Vitro Study to Evaluate and Compare the Hemocompatibility of Titanium and Zirconia Implant Materials after Sandblasted and Acid Etched Surface Treatment

Sujith Sivarajan, Arjun Rajan, Anjana S Nair, Shilpa Jayakumar, Arya S Pillai

Keywords : Acid etching, Hemocompatibility, Sandblasting, titanium, Zirconia

Citation Information : Sivarajan S, Rajan A, Nair AS, Jayakumar S, Pillai AS. An In Vitro Study to Evaluate and Compare the Hemocompatibility of Titanium and Zirconia Implant Materials after Sandblasted and Acid Etched Surface Treatment. J Contemp Dent Pract 2018; 19 (12):1449-1455.

DOI: 10.5005/jp-journals-10024-2448

License: CC BY-NC 4.0

Published Online: 01-12-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: This study was aimed to investigate the hemocompatibility of zirconia and titanium implant materials after surface treatment with sandblasting and acid etching (SLA). Materials and methods: Sixty specimens were procured from manufacturers of dimension 10mm x 3mm, thirty of each were prefabricated medical grade titanium (Ti-6Al-4V) and thirty of sintered zirconia. Silicon carbide grit papers of 240 to 1200ìm, was used to polish the specimen surface. The surfaces were rinsed with water to remove any remnant particles after polishing. Later ultrasonic cleaning was done for 5 minutes using distilled water. The control specimens included 15 specimens each from titanium (groups A1) and zirconia (groups B1). The remaining 15 specimens (groups A2 and B2) were sandblasted using alumina particles of 150 microns particle size and using 20% hydrochloric acid, acid etching was done for 30 seconds. The specimens were scanned under electron microscope after surface treatment for analysis purpose and evaluated for surface characteristics. Before the exposure of specimens to blood, percentage hemolysis, prothrombin, platelet aggregation and activation, and thrombin time values were calculated. 1 ml of blood was added to each specimen for testing. The values before and after the exposure of specimens to blood were noted. Using a t-test, the values noted were statistically evaluated. Results: A1 (polished titanium) showed highest mean values after exposure, in platelet count (184.67 ± 1.29), Leucocyte count (7.27 ± 0.08), and Thrombin time (10.15 ± 0.34) while Prothrombin time\'s highest mean value after exposure were showed by A2 (SLA treated titanium) with a mean value of 10.04 ± 0.24. Conclusion: Surface treatment with sandblasting and acid etching (SLA) using 150 microns alumina particles and 20% hydrochloric acid increased the surface roughness of the titanium and zirconia implant materials and polished titanium showed maximum hemocompatibility. Clinical significance: The implant\'s success depends on its biocompatibility and its property of osseointegration. The adverse interaction between blood and the artificial surface is detected by the hemocompatibility test for medical materials, to know if the surface can activate or destruct the blood components. The success of implant placement also depends on the interaction between the blood and the specimen.


PDF Share
  1. Cochran DL, Schenk RK, Lussi A, Higginbottom FL, Buser D. Bone response to unloaded and loaded titanium implants with a sandblasted and acid-etched surface: A histometric study in the canine mandible. J Biomed Mater Res. 1998; 40:1-11.
  2. Maeztu MA, Alava JI, Escoda CG. Ion implantation: Surface treatment for improving the bone integration of titanium and Ti6Al4V dental implants. Clin Oral Impl Res. 2003; 14:57-62.
  3. Mitsuru Takemoto, Fujibayashi S, Neo M, Suzuki J, Matsushita T, Kokubo T, Nakamura T. Osteoinductive porous titanium implants: Effect of sodium removal by dilute HCl treatment. Biomaterials. 2006; 27:2682-2691.
  4. Bhavanchand Y, Ranzani R, Annapoorani H. Evaluation of hemocompatibility of titanium after various surface treatments: An in vitro Study. Int J Prosthodont Restor Dent. 2012; 2(4):136-142.
  5. Murray PE, Godoy CG, Godoy FG. How is the biocompatibilty of dental biomaterials evaluated? Med Oral Patol Oral Cir Bucal. 2007; 12:258-66.
  6. Sanak M, Jakieła B, Wêgrzyn W. Assessment of hemocompatibility of materials with arterial blood flow by platelet functional tests. B POL ACAD SCI-TECH. 2010; 58(2):317-322.
  7. Sul YT, Johansson CB, Petronis S, Krozer A, Jeong Y, Wennerberg A, et.al. Characteristics of the surface oxides on turned and electrochemically oxidized pure titanium implants up to dielectric breakdown: the oxide thickness, micropore configurations, surface roughness, crystal structure and chemical composition. Biomaterials. 2002; 23:491-501.
  8. Nan H, Ping Y, Xuan C, Yongxang L, Xiaolan Z, Guangjun C et.al. Blood compatibility of amorphous titanium oxide films synthesized by ion beam enhanced deposition. Biomaterials. 1998; 19:771-776.
  9. Gahlert M, Rohling S, Wieland M, Eichhorn S, Kuchenhoff H, Kniha H. A Comparison Study of the Osseointegration of Zirconia and Titanium Dental Implants. A Biomechanical Evaluation in the Maxilla of Pigs. Clin Implant Dent Relat Res. 2010; 12(4):297-305.
  10. Hyeongil Kim, Choi SH, Ryu JJ, Koh YS, Park JH, Lee IS. The biocompatibility of SLA-treated titanium implants. Biomed. Mater. 2008; 3:1-6
  11. Packham MA. The behavior of platelets at foreign surfaces. Proc Soc Exp Biol Med. 1988; 189:261-274
  12. Kang SJ, Kim BM, Lee YJ, Hong SH, Chung HW. Titanium dioxide nanoparticles induce apoptosis through the JNK/ p38-caspase-8-Bid pathway in phytohemagglutinin-stimulated human lymphocytes. Biochem. Cell Biol. 2009;386: 682-687
  13. Schreiber BG, Neubert A, Muller WD, Hopp M, Griepentrog M, Lange KP. Fibroblast growth on surface-modified dental implants: An in vitro study. J Biomed Mater Res. 2003; 64:591-599.
  14. Depprich R, Zipprich H, Ommerborn M, Mahn E, Lammers L, Handschel J et.al. Osseointegration of zirconia implants: an SEM observation of the bone-implant interface. Head Face Med. 2008; 4(25):1-7.
  15. Li Y, Zou S, Wang D, Feng G, Bao C, Hu J. The effect of hydrofluoric acid treatment on titanium implant osseointegration in ovariectomized rats. Biomaterials. 2010; 31:3266-3273.
  16. Zinelis S, Thomas A, Syres K, Silikas N, Eliadesa G. Surface characterization of zirconia dental implants. Dent Mater. 2010; 26:295-305.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.