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VOLUME 19 , ISSUE 8 ( 2018 ) > List of Articles

ORIGINAL RESEARCH

Effect of Radiotherapy on Cariogenic Organism Streptococcus sobrinus in Saliva in Head and Neck Cancer: A Clinical Study

Renu Batra, Sonali Kapoor, Shilpi R Daveshwar, Meena R Daveshwar, Vineet Agrawal

Keywords : Dental caries, Oral cancer, Polymerase chain reaction.

Citation Information : Batra R, Kapoor S, Daveshwar SR, Daveshwar MR, Agrawal V. Effect of Radiotherapy on Cariogenic Organism Streptococcus sobrinus in Saliva in Head and Neck Cancer: A Clinical Study. J Contemp Dent Pract 2018; 19 (8):929-932.

DOI: 10.5005/jp-journals-10024-2359

License: CC BY-NC 3.0

Published Online: 01-08-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: Aim of the study was to assess salivary Streptococcus sobrinus in head and neck cancer using quantitative polymerase chain reaction (PCR). Materials and methods: Unstimulated saliva samples were collected from head and neck cancer patient preradiotherapy. Unstimulated saliva samples were collected from oral and laryngeal cancer patients after 6 weeks of radiotherapy (dose 60 Gy). The subjects were explained not to consume solids or liquids or carry out any dental hygiene activity 1 hour prior to saliva collection. Accumulated unstimulated saliva was collected in cylindrical tube through funnel. The collected saliva was then transferred to Eppendorf tube containing Tris–ethylenediaminetetraacetic acid (EDTA) (TE) buffer and was transported to lab for real-time PCR analysis. Results: Streptococcus sobrinus significantly increased postradiotherapy as compared with preradiotherapy in head and neck cancer patients. Conclusion: Within the limitation of this study, we conclude that amount of S. sobrinus increases postradiotherapy in head and neck cancer patients. Clinical significance: As radiation therapy has harmful effects on hard and soft tissues of oral cavity, dentists should provide motivation for oral health care to the patients.


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