Citation Information :
Vikhe DM, Mastud CS, Mantri T, Mhaske PN, Mastud SP. Staining the Ground Section of the Tooth Using an Innovative Plant Stain Found in the Pravara Region, India. J Contemp Dent Pract 2020; 21 (10):1113-1116.
Aims: To assess the staining efficacy of the innovative “Pravara” stain on ground sections of human teeth under a stereomicroscope. In this study, the ground sections were stained with the innovative stain. The stain is obtained from a leafless tree called Terminalia chebula. Terminalia chebula, commonly known as Harad, is found in South Asia from India. Few species are found in the Pravara region of Maharashtra, India. Materials and methods: A total of 40 extracted teeth were collected and ground sections of each tooth were prepared. The samples were randomly divided into two groups: group I—control (without stain), group II—study group (the samples in this group were stained with the help of innovative “Pravara” stain). The sections were graded from I to III according to the differentiation and contrast of tissues. Results: The ground sections in group II stained with Pravara stain demonstrated better contrast for structures within sections under a microscope in comparison with group I (control group). Conclusion: The study demonstrates the efficacy of a new innovative Pravara stain, which is a simple, economical, quick, and effective staining method for the ground sections of the teeth. Clinical significance: This innovative Pravara stain is cost-effective, saves time, ecofriendly, and highlights the anatomical structures more effectively and does not fade easily with time.
Tan N, Thentamil A, Jacob M. Hard tissue architecture of tooth—no strain with stain. J Acad Dent Educ 2017;3(1):5–9. DOI: 10.18311/jade/2017/16448.
Lakshmi TA, Sumitra V, Victor R. Application of MV10B stain for paraffin sections of teeth. Int J Health Sci Res 2013;3(8):17–21.
Brewer HE, Shellhamer RH. Stained ground sections of teeth and bone. Stain Technol 1956;31(3):111–114. DOI: 10.3109/10520295609113787.
“Terminalia chebula Retz”. Germplasm Resources Information Network (GRIN). Agricultural Research Service (ARS), United States Department of Agriculture (USDA). Retrieved 6 Aug 2016.
Charles DK, Condon K, Cheverud JM, et al. Cementum annulation and age determination in homo sapiens. I. tooth variability and observer error. Am J Physical Anthropol 1986;71(3):311–320. DOI: 10.1002/ajpa.1330710306.
Gibb T, Oseto C. Arthropod Collection and Identification: Laboratory and Field Techniques. San Diego, California: Elsevier Academic Press Publications; 2006. p. 75.
Shukla D, Vinuth DP, Sowmya SV, et al. Cementum made more Visual. J Forensic Odontostomatol 2012;30(1):29–37.
Yadav SM, Wakode R, Kumar S, et al. Ground sections of teeth: histopathological study modality. Int J Res Med Sci 2019;7(4): 1384–1387. DOI: 10.18203/2320-6012.ijrms20191359.
Prasad BK, Kumar P, Tyagi YK. Age determination: in relation to specific demands of forensic practice. Anil Aggrawal's internet. J Forensic Med Toxicol 2003;4(2):4–7.
Charles DK, Condon K, Cheverud JM, et al. Estimating age at death from growth layer groups in cementum. In: Iscan MY, Kennedy KAR. Age Markers in the Human Skeleton. Springfield: Charles C. Thomas; 1989. pp. 277–316.
Robbins G, Lukacs JR. Cementum annulations and age estimation in an early holocene population. J Odondol 2004;13:212–227.
Little MF, Cueto CS, Rowley J. Chemical and physical properties of altered and sound enamel. I.ASH, ca, P, CO2, N, water, microradiolucency and density. Arch Oral Biol 1962;7:173–184. DOI: 10.1016/0003-9969(62)90005-5.
Alturkistani HA, Tashkandi FM, Mohammedsaleh ZM. Histological stains: a literature review and case study. Glob J Health Sci 2015;8(3):72–79. DOI: 10.5539/gjhs.v8n3p72.
Lillie RD, Pizzolato P, Donaldson PT. Hematoxylin substitutes. A study of phenocyanin TC and the use of after chromemordanting in histology. Am J Clin Pathol 1975;63(6):876–885.
Lillie RD, Pizzolato P, Welsh RA, et al. A consideration of substitutes for alum hematoxylin in routine histologic and cytologic diagnostic procedures. Am J Clin Path 1973;60(6):817–819. DOI: 10.1093/ajcp/60.6.817.
Lillie RD, Pizzolato P, Donaldson PT. Hematoxylin substitutes: a survey of mordant dyes tested and consideration of the relation of their structure to performance as nuclear stains. Stain Technol 1976;51(1):25–41. DOI: 10.3109/10520297609116665.
Lillie RD, Pizzolato P, Donaldson PT. Hematoxylin substitutes: fluorone black and methyl fluoroneblack (9-phenyl- and 9-methyl-2,3,7-trihydroxy-6-fluorone) as metachrome iron alum mordantdyes. Stain Technol 1975;50(2):127–131. DOI: 10.3109/10520297509117046.
Hogg RM, Simpson R. An evaluation of solochrome cyanine R.S. as a nuclear stain similar to haematoxylin. Med Lab Technol 1975;32(4):301–306.
Llewellyn BD. Mordant blue 3: a readily available substitute for hematoxylin in the routine hematoxylin and eosin stain. Stain Technol 1974;49(6):347–349. DOI: 10.3109/10520297409117010.
Insira Sarbeen J, Jayaraj G. Light microscopic study of cementum under different histological stains. J Pharm Sci Res 2015;7(9): 720–723.
Ramamoorthy A, Ravi S, Jeddy N, et al. Natural alternatives for chemicals used in histopathology lab: a literature review. J Clin Diagn Res 2016;10(11):EE01–EE04. DOI: 10.7860/JCDR/2016/23420. 8860.
Tewari D, Mocan A, Parvanov ED, et al. Ethnopharmacological approaches for therapy of jaundice: part II. highly used plant species from Acanthaceae, Euphorbiaceae, Asteraceae, Combretaceae, and Fabaceae families. Front Pharmacol 2017;8:519. DOI: 10.3389/fphar.2017.00519.
Han Q, Song J, Qiao C, et al. “Preparative isolation of hydrolysable tannins chebulagic acid and chebulinic acid from Terminalia chebula by high-speed counter-current chromatography” (PDF). J Sep Sci 2006;29(11):1653–1657. DOI: 10.1002/jssc.200600089.