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VOLUME 21 , ISSUE 11 ( November, 2020 ) > List of Articles


Effect of Calcium Hydroxide Combinations with Green Tea Extract and Cocoa Pod Husk Extract on p38 MAPK and Reparative Dentine

Tamara Yuanita, Irma Drismayanti, Deavita Dinari, Lailatun Tedja

Citation Information : Yuanita T, Drismayanti I, Dinari D, Tedja L. Effect of Calcium Hydroxide Combinations with Green Tea Extract and Cocoa Pod Husk Extract on p38 MAPK and Reparative Dentine. J Contemp Dent Pract 2020; 21 (11):1238-1244.

DOI: 10.5005/jp-journals-10024-2950

License: CC BY-NC 4.0

Published Online: 01-04-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Aim and objective: The aim of this research is to analyze the effect of calcium hydroxide combinations with green tea extract and the combination of calcium hydroxide with cocoa pod husk extract on the activation of p38 MAPK and wide area of reparative dentin in mice dental. Materials and methods: This study used 36 rats that were randomly divided into three treatment groups: positive control group was applied calcium hydroxide and aquades (group I), the test group was applied calcium hydroxide combined with cocoa pod husk extract (group II), and the next test group was applied using calcium hydroxide combined with green tea extract (group III); all the cavities were restored with RMGIC. On day 7 and 28, experimental animals from each treatment group were killed by peritoneal injection to see the activation of p38 MAPK, while reparative dentin was only seen on day 28. Results: The result of data analysis using Multiple Comparison Tukey HSD test showed significant difference between the positive control group and the test groups for the average p38 MAPK activation value on day 7 and 28. But there was no significant difference between two test groups. The same thing was obtained in the calculation of the average area of reparative dentin, where group I had the lowest value compared to groups II and III on day 28 with a significant difference. There was no significant difference between groups II and III. Conclusion: The use of combination calcium hydroxide with green tea extract and combination calcium hydroxide with cocoa pod husk extract have significant effect on p38 MAPK activation and wide area of reparative dentin in mice dental. Clinical significance: The use of combination calcium hydroxide with green tea extract and combination calcium hydroxide with cocoa pod husk extract have been proven to activate more p38 and form a wider reparative dentin.

  1. Inflammation and regeneration in the dentin-pulp complex: a double-edged sword. J Endod 2014;40(4):S46–S51. DOI: 10.1016/j.joen.2014.01.021.
  2. Ability of stem and progenitor cells in the dental pulp to hard tissue form. Japan Dent Sci Rev 2015;51(3):75–83. DOI: 10.1016/j.jdsr.2015.03.002.
  3. Activation and signaling of the p38 MAP kinase pathway. Cell Res 2005;15(1):11–18. DOI: 10.1038/
  4. A comparative histological analysis of human pulp following direct pulp capping with propolis mineral trioxide aggregate and Dycal. Austral Dent J 2010;55(1):59–64. DOI: 10.1111/j.1834-7819.2009.01179.x.
  5. Keys to clinical success with pulp capping: a review of the literature. Oper Dent 2009;34(5):615–625. DOI: 10.2341/09-132-0.
  6. Mineral trioxide aggregate or calcium hydroxide direct pulp capping: An analysis of the clinical treatment outcome. J Endod 2010;36(5):806–813. DOI: 10.1016/j.joen.2010.02.024.
  7. Induction of reparative dentin formation on exposed dental pulp by dentin phosphophoryn/collagen composite. Bio Med Res Int 2014;2014:40–49. 745139 10.1155/2014/745139.
  8. Why honey is effective as a medicine. Part 2. The scientific explanation of its effects. Bee World 2001;82(1):22–40. DOI: 10.1080/0005772X.2001.11099498.
  9. The potential of cocoa (Theobroma cacao L.) pods extract in periodontal dressing to rabbit gingival wound healing. 2015;10:24–25.
  10. Physicochemical and antimicrobial properties of cocoa pod husk pectin intended as a versatile pharmaceutical excipient and nutraceutical. J Pharmaceut 2016. 7608693. DOI:
  11. Cocoa polyphenols and their potential benefits for human health. Oxidat Med Cell Longev 2012. 906252. DOI: 10.1155/2012/906252.
  12. Effects of cocoa products and its polyphenolic constituents on exercise performance and exercise-induced muscle damage and inflammation: a review of clinical trials. Nutrients 2019;11(7):1471. DOI: 10.3390/nu11071471.
  13. Antioxidant activity and phenolic content of different pod tissues of five selected cocoa hybrid lines. J Food Agricult 2012;5(1-2):5–12. DOI: 10.4038/jfa.v5i1-2.5177.
  14. The immunomodulatory and anti-inflammatory role of polyphenols. Nutrients 2018;10(11):1618. DOI: 10.3390/nu10111618.
  15. Treatment outcome of vital pulp treatment. Endodont Top 2002;2(1):24–34. DOI: 10.1034/j.1601-1546.2002.20103.x.
  16. Cocoa polyphenols prevent inflammation in the colon of azoxymethane-treated rats and in TNF-α-stimulated Caco-2 cells. Br J Nutrit 2013;110(2):206–215. DOI: 10.1017/S0007114512004862.
  17. Cocoa polyphenols suppress TNF-α-induced vascular endothelial growth factor expression by inhibiting phosphoinositide 3-kinase (PI3K) and mitogenactivated protein kinase kinase-1 (MEK1) activities in mouse epidermal cells. Br J Nutrit 2010;104(7):957–964. DOI: 10.1017/S0007114510001704.
  18. Cocoa (Theobroma cacao L) pod husk: renewable source of bioactive compounds. Trends Food Sci Technol 2018;81:172–184. DOI:
  19. Antioxidant activity of phenolic extracts from mangosteen (Garcinia mangostana L.) J Mathemat Nat Sci 2012.
  20. Green tea (−)-epigallocatechin gallate inhibits the growth of human villous trophoblasts via the ERK, p38, AMP-activated protein kinase, and protein kinase B pathways. Am J Physiol Cell Physiol 2016;311(2):C308–C321. DOI: 10.1152/ajpcell.00003.2016.
  21. Insulin-like growth factor 1 receptor and p38 mitogen-activated protein kinase signals inversely regulate signal transducer and activator of transcription 3 activity to control human dental pulp stem cell quiescence, propagation, and differentiation. Stem Cells Develop 2014;23(8):839–851. DOI: 10.1089/scd.2013.0400.
  22. Magnetic nanocomposite scaffold-induced stimulation of migration and Odontogenesis of human dental pulp cells through Integrin signaling pathways. PLoS ONE 2015;10(9):e0138614. DOI: 10.1371/journal.pone.0138614.
  23. Use of 0.4-Tesla static magnetic field to promote reparative dentine formation of dental pulp stems cells through activation of p38 MAPK signaling pathway. Int Endodon J 2018. DOI: 10.1111/iej.12962.
  24. IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling. Scient Rep 2017;7(1):40681. DOI: 10.1038/srep40681.
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