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VOLUME 21 , ISSUE 6 ( June, 2020 ) > List of Articles


Effect of Intracanal Medicaments (Modified Triple Antibiotic Paste, Calcium Hydroxide, and Aloe Vera) on Microhardness of Root Dentine: An In Vitro Study

Vijay Parashar

Citation Information : Parashar V. Effect of Intracanal Medicaments (Modified Triple Antibiotic Paste, Calcium Hydroxide, and Aloe Vera) on Microhardness of Root Dentine: An In Vitro Study. J Contemp Dent Pract 2020; 21 (6):632-635.

DOI: 10.5005/jp-journals-10024-2813

License: CC BY-NC 4.0

Published Online: 04-11-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Aim: To compare the effect of three different intracanal medicaments, namely, modified triple antibiotic paste (MTAP), calcium hydroxide (Ca(OH)2), and aloe vera, on the root dentine microhardness. Materials and methods: A total of 50 extracted mandibular bicuspids were prepared using ProTaper Next rotary files. The roots of the bicuspids were alienated to three groups (n = 10 each) and one control group (untreated; n = 20). In three groups, the root canals were filled with MTAP, Ca(OH)2, and aloe vera medicaments. After 21 days, medicaments were removed by Endo activator. Mean Knoop hardness numbers were calculated after treatment and compared with the untreated control group. Data were evaluated using the Student's t test (paired), ANOVA (one-way) followed, and the post hoc test. Results: All treated groups except the aloe vera group had shown significant reduction (p < 0.05) in microhardness of the root dentin as compared with the untreated control group. The aloe vera group showed least reduction of microhardness and was statistically insignificant (p > 0.05). Conclusion: Aloe vera shows promising results in terms of fewer effects on microhardness of the root dentin compared to MTAP and Ca(OH)2. Clinical significance: Elimination of most of the bacterial infection from the root canal and very minimum to no effect on the microhardness of the dentin in the root part are the basics of success in any endodontic treatment. Further in vivo studies are required to compare the efficacy of these intracanal medicaments.

  1. Vijayaraghavan R, Mathian VM, Sundaram AM, et al. Triple antibiotic paste in root canal therapy. J Pharm Bioallied Sci 2012;4(2):230–233. DOI: 10.4103/0975-7406.100214.
  2. Yaduka P, Sharma S. Novel intracanal medicaments and its future scope. Int J Pharm Bio Sci 2014;4(3):65–69.
  3. Yoldas O, Dogan C, Seydaoglu G. The effect of two different calcium hydroxide combinations on root dentine microhardness. Int Endod J 2004;37(12):828–831. DOI: 10.1111/j.1365-2591.2004.00885.x.
  4. Bansal R, Jain A. Overview on the current antibiotic containing agents used in endodontics. North american. J Med Sci 2014;6(8):351–358.
  5. Athanassiadis B, Abbott PV, Walsh LJ. The use of calcium hydroxide, antibiotics and biocides as antimicrobial medicaments in endodontics. Aust Dent J 2007;52(1 Suppl):S64–S82. DOI: 10.1111/j.1834-7819.2007.tb00527.x.
  6. Abdel Hamid E, Abdel Aziz S, Sadek HS, et al. Effectiveness of triple antibiotic paste as an intra-canal medication for the root canal treatment of non-vital teeth with apical periodontitis: a systematic review. F1000Res 2018;7:1627. DOI: 10.12688/f1000research.16423.1.
  7. Zargar N, Rayat Hosein Abadi M, Sabeti M, et al. Antimicrobial efficacy of clindamycin and triple antibiotic paste as root canal medicaments on tubular infection: an in vitro study. Aust Endod J 2019;45(1):86–91. DOI: 10.1111/aej.12288.
  8. Rosenberg B, Murray PE, Namerow K. The effect of calcium hydroxide root filling on dentin fracture strength. Dent Traumatol 2007;23(1): 26–29. DOI: 10.1111/j.1600-9657.2006.00453.x.
  9. Andreasen JO, Farik B, Munksgaard EC. Long-term calcium hydroxide as a root canal dressing may increase risk of root fracture. Dent Traumatol 2002;18(3):134–137. DOI: 10.1034/j.1600-9657.2002.00097.x.
  10. Yassen GH, Platt JA. The effect of nonsetting calcium hydroxide on root fracture and mechanical properties of radicular dentine: a systematic review. Int Endod J 2013;46(2):112–118. DOI: 10.1111/j.1365-2591.2012.02121.x.
  11. Prather BT, Ehrlich Y, Spolnik K, et al. Effects of two combinations of triple antibiotic paste used in endodontic regeneration on root microhardness and chemical structure of radicular dentine. J Oral Sci 2014;56(4):245–251. DOI: 10.2334/josnusd.56.245.
  12. Kurian B, Swapna DV, Nadig RR, et al. Efficacy of calcium hydroxide, mushroom, and aloe vera as an intracanal medicament against enterococcus faecalis: an in vitro study. Endodontology 2016;28(2):137–142. DOI: 10.4103/0970-7212.195427.
  13. Prashar P, Gulati S, Koul V, et al. In vitro antibacterial activity of ethanolic extract of aloe vera against some bacterial and fungal species. J Adv Biotech 2011;11:3–8.
  14. Meena M, Figueiredo NR, Trivedi K. Aloe vera – an update for dentistry. Journal of Dentofacial Sciences 2013;2(4):1–4.
  15. Neena IE, Ganesh E, Poornima P, et al. An ancient herb aloevera in dentistry: A review. J Oral Res Rev 2015;7(1):25–30. DOI: 10.4103/2249-4987.160174.
  16. Akman M, Akbulut MB, Aydınbelge HA, et al. Comparison of different irrigation activation regimens and conventional irrigation techniques for the removal of modified triple antibiotic paste from root canals. J Endod 2015;41(5):720–724. DOI: 10.1016/j.joen.2015.01.001.
  17. Vasudeva A, Sinha DJ, Tyagi SP, et al. Disinfection of dentinal tubules with 2% chlorhexidine gel, calcium hydroxide and herbal intracanal medicaments against enterococcus faecalis: an in-vitro study. Singapore Dent J 2017;38:39–44. DOI: 10.1016/j.sdj.2017.06.001.
  18. Leiendecker AP, Qi YP, Sawyer AN, et al. Effects of calcium silicate-based materials on collagen matrix integrity of mineralized dentin. J Endod 2012;38(6):829–833. DOI: 10.1016/j.joen.2012.01.004.
  19. Sinha DJ, Jaiswal N, Vasudeva A, et al. Comparative evaluation of the effect of chlorhexidine and aloe barbadensis miller (aloe vera) on dentin stabilization using shear bond testing. J Conserv Dent 2016;19(5):406–409. DOI: 10.4103/0972-0707.190017.
  20. Bertolini PF, Biondi Filho O, Pomilio A, et al. Antimicrobial capacity of aloe vera and propolis dentifrice against streptococcus mutans strains in toothbrushes: an in vitro study. J Appl Oral Sci 2012;20(1):32–37. DOI: 10.1590/s1678-77572012000100007.
  21. Kudalkar MD, Nayak A, Bhat KS, et al. Effect of Azadirachta indica (neem) and aloe vera as compared to subantimicrobial dose doxycycline on matrix metalloproteinases (MMP)-2 and MMP-9: an in-vitro study. Ayu 2014;35(1):85–89. DOI: 10.4103/0974-8520.141947.
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