Salivary Immunoglobulin A and Streptococcus mutans Levels among Lebanese Preschool Children with Early Childhood Caries
Mona Nahas, Elia Sfeir
Early childhood caries, Immunoglobulin A, Prevention, Streptococcus mutans
Citation Information :
Nahas M, Sfeir E. Salivary Immunoglobulin A and Streptococcus mutans Levels among Lebanese Preschool Children with Early Childhood Caries. J Contemp Dent Pract 2020; 21 (9):1012-1017.
Aim and objective: We checked in this study the correlation between total immunoglobulin A (IgA) and Streptococcus mutans (SM) levels in saliva derived from Lebanese children with inappropriate eating habits and showing early childhood caries (ECC). Materials and methods: Sixty Lebanese preschool children with similar alimentation were included in this study and divided into two groups. Group I included children having 0 cavities where group II contained children having ECC. We measured the SM and IgA levels collected from saliva and dental plaque. Results: We observed a significant difference in SM levels between the two groups (p < 0.001). There was a marginal correlation between salivary total IgA and SM collected from dental plaque (r = 0.33, p = 0.077). However, no significant correlation was detected between total salivary IgA and salivary SM (p = 0.35). Conclusion: This study suggests the absence of significant relationship between salivary markers of immune system and the development of ECC. Clinical significance: Early childhood caries is a public dental health problem that has been affecting preschool children all over the world. Its prevention must be a priority for all professionals in the medical and dental community. This study highlights the absence of correlation between total IgA and SM collected from the saliva. This does not completely exclude the preventive role of salivary immune components, but further studies are required to better understand this relationship.
Shore RC, Kirkham J, Brookes SJ, et al. Distribution of exogenous proteins in caries lesions in relation to the pattern of demineralisation. Caries Res [Internet] 2000;34(2):188–193. DOI: 10.1159/000016588. Available from http://www.ncbi.nlm.nih.gov/pubmed/10773638.
El Tantawi M, Folayan MO, Mehaina M, et al. Prevalence and data availability of early childhood caries in 193 United Nations countries, 2007-2017. Am J Public Health [Internet] American Public Health Association; 2018;108(8):1066–1072. Available from https://ajph.aphapublications.org/doi/10.2105/AJPH.2018.304466.
Finlayson TL, Gupta A, Ramos-Gomez FJ. Prenatal maternal factors, Intergenerational transmission of disease, and child oral health outcomes. Dent Clin North Am [Internet] 2017;61(3):483–518. DOI: 10.1016/j.cden.2017.02.001. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28577633.
Ganesh A, Muthu MS, Mohan A, et al. Prevalence of early childhood caries in india – a systematic review. Indian J Pediatr [Internet] 2019;86(3):276–286. Available from http://link.springer.com/10.1007/s12098-018-2793-y.
Seow WK. Early childhood caries. Pediatr Clin North Am [Internet] 2018;65(5):941–954. DOI: 10.1016/j.pcl.2018.05.004. Available from http://www.ncbi.nlm.nih.gov/pubmed/30213355.
Warren JJ, Weber-Gasparoni K, Marshall TA, et al. Factors associated with dental caries experience in 1-year-old children. J Public Health Dent [Internet] 2008;68(2):70–75. Available from: http://doi.wiley.com/10.1111/j.1752-7325.2007.00068.x.
Chedid NR, Bourgeois D, Kaloustian H, et al. Caries Prevalence and caries risk in a sample of Lebanese preschool children. Odontostomatol Trop [Internet] 2011;34(134):31–45. Available from http://www.ncbi.nlm.nih.gov/pubmed/22003807.
Anil S, Anand PS. Early childhood caries: Prevalence, risk factors, and prevention. Front Pediatr [internet] Frontiers Media SA; 2017;5:157. DOI: 10.3389/fped.2017.00157. Available from http://www.ncbi.nlm.nih.gov/pubmed/28770188.
Tinanoff N. Introduction to the early childhood caries conference: initial description and current understanding. Commun Dent Oral Epidemiol [Internet] 1998;26(S1):5–7. Available from: http://doi.wiley.com/10.1111/j.1600-0528.1998.tb02089.x.
Caplan LS, Erwin K, Lense E, et al. The potential role of breast-feeding and other factors in helping to reduce early childhood caries. J Public Health Dent [Internet] 2008;68(4):238–241. DOI: 10.1111/j.1752-7325.2007.00080.x. Available from http://www.ncbi.nlm.nih.gov/pubmed/18384535.
Russell MW, Hajishengallis G, Childers NK, et al. Secretory immunity in defense against cariogenic Mutans Streptococci. Caries Res [Internet] 1999;33(1):4–15. DOI: 10.1159/000016490. Available from http://www.ncbi.nlm.nih.gov/pubmed/9831775.
Michalek SM, Katz J, Childers NK. A vaccine against dental caries. BioDrugs [Internet] 2001;15(8):501–508. DOI: 10.2165/00063030-200115080-00002. Available from http://www.ncbi.nlm.nih.gov/pubmed/11543691.
Gonçalves de Farias D, Barreto Bezerra AC. Salivary antibodies, amylase and protein from children with early childhood caries. Clin Oral Investig [Internet] 2003;7(3):154–157. DOI: 10.1007/s00784-003-0222-7. Available from http://www.ncbi.nlm.nih.gov/pubmed/12915963.
Alaluusua S. Longitudinal study of salivary IgA in children from 1 to 4 years old with reference to dental caries. Eur J Oral Sci [Internet]. John Wiley & Sons, Ltd 1983;91(3):163–168. Available from: http://doi.wiley.com/10.1111/j.1600-0722.1983.tb00796.x.
Rose PT, Gregory RL, Gfell LE, et al. IgA antibodies to Streptococcus mutans in caries-resistant and -susceptible children. Pediatr Dent [Internet] 2020;16(4):272–275. Available from: http://www.ncbi.nlm.nih.gov/pubmed/7937259.
Vadiakas G. Case definition, aetiology and risk assessment of early childhood caries (ECC): a revisited review. Eur Arch Paediatr Dent [Internet] 2008;9(3):114–125. DOI: 10.1007/BF03262622. Available from http://www.ncbi.nlm.nih.gov/pubmed/18793593.
Kaste LM, Drury TF, Horowitz AM, et al. An Evaluation of NHANES III estimates of early childhood caries. J Public Health Dent [Internet] 1999;59(3):198–200. Available from: http://doi.wiley.com/10.1111/j.1752-7325.1999.tb03269.x.
Folayan M, Olatubosun S. Early childhood caries - a diagnostic enigma. Eur J Paediatr Dent [Internet] 2018;19(2):88. DOI: 10.23804/ejpd.2018.19.02.00. Available from http://www.ncbi.nlm.nih.gov/pubmed/30063145.
Kirthiga M, Murugan M, Saikia A, et al. Risk factors for early childhood caries: a systematic review and Meta-analysis of case control and cohort studies. Pediatr Dent [Internet] 2019;41(2):95–112. Available from http://www.ncbi.nlm.nih.gov/pubmed/30992106.
Miyaji H, Azuma T, Ito S, et al. Helicobacter pylori infection occurs via close contact with infected individuals in early childhood. J Gastroenterol Hepatol 2000;15(3):257–262. DOI: 10.1046/j.1440-1746.2000.02070.x.
Sun HB, Zhang W, Zhou XB. Risk factors associated with early childhood caries. Chin J Dent Res [Internet] 2017;20(2):97–104. Available from http://www.ncbi.nlm.nih.gov/pubmed/28573263.
Schroth RJ, Cheba V. Determining the prevalence and risk factors for early childhood caries in a community dental health clinic. Pediatr Dent [Internet] 2007;29(5):387–396. Available from http://www.ncbi.nlm.nih.gov/pubmed/18027773.
Tiberia MJ, Milnes AR, Feigal RJ, et al. Risk factors for early childhood caries in Canadian preschool children seeking care. Pediatr Dent [Internet] 2007;29(3):201–208. Available from http://www.ncbi.nlm.nih.gov/pubmed/17688016.
Seki M, Karakama F, Terajima T, et al. Evaluation of mutans streptococci in plaque and saliva: correlation with caries development in preschool children. J Dent [Internet] 2003;31(4):283–290. DOI: 10.1016/s0300-5712(03)00033-2. Available from http://www.ncbi.nlm.nih.gov/pubmed/12735923.
Tedjosasongko U, Kozai K. Initial acquisition and transmission of Mutans Streptococci in children at day nursery. ASDC J Dent Child [Internet] ASDC J Dent Child 2002;69(3). Available from: https://pubmed.ncbi.nlm.nih.gov/12613313/?from_term=Tedjosasongko+U&from_pos=3.
SJ W, GZ W. Variables influencing Streptococcus Mutans testing. Pediatr Dent [Internet]. Pediatr Dent 1990;12(5). Available from: https://pubmed.ncbi.nlm.nih.gov/2095541/?from_term=Weinberger+SJ&from_cauthor_id=2095541&from_pos=1.
Smith DJ, King WF, Akita H, et al. Association of salivary immunoglobulin A antibody and initial Mutans Streptococcal infection. Oral Microbiol Immunol [Internet] Oral Microbiol Immunol 1998;13(5). Available from: https://pubmed.ncbi.nlm.nih.gov/9807119/?from_term=Smith+DJ&from_cauthor_id=9807119&from_pos=1.
Bosch JA, Ring C, de Geus EJ, et al. Stress and secretory immunity. Int Rev Neurobiol [Internet] Int Rev Neurobiol 2002;52:213–253. DOI: 10.1016/s0074-7742(02)52011-0. Available from: https://pubmed.ncbi.nlm.nih.gov/12498106/?from_term=Bosch+JA&from_cauthor_id=12498106&from_pos=1.
Tanabe Y, Park JH, Tinanoff N, et al. Comparison of chairside microbiological screening systems and conventional selective media in children with and without visible dental caries. Pediatr Dent [Internet] 2006;28(4):363–368. Available from http://www.ncbi.nlm.nih.gov/pubmed/16903447.
Becker MR, Paster BJ, Leys EJ, et al. Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol [Internet] 2002;40(3):1001–1009. Available from http://jcm.asm.org/cgi/doi/10.1128/JCM.40.3.1001-1009.2002.
van Everdingen T, Eijkman MA, Hoogstraten J. Parents and nursing-bottle caries. ASDC J Dent Child [Internet] 63(4):271–274. Available from http://www.ncbi.nlm.nih.gov/pubmed/8893981.
Febres C, Echeverri EA, Keene HJ. Parental awareness, habits, and social factors and their relationship to baby bottle tooth decay. Pediatr Dent [Internet] 19(1):22–27. Available from http://www.ncbi.nlm.nih.gov/pubmed/9048409.
Elarabi S, Elhamid A, Harchaoui J, et al. La polycarie infantile: facteurs de risque. J Dent du Québec 2001;37:363–371.
Occhi-Alexandre IGP, Cruz PV, Bendo CB, et al. Prevalence of dental caries in preschool children born preterm and/or with low birth weight: A systematic review with meta-analysis of prevalence data. Int J Paediatr Dent [Internet] 2020;30(3):265–275. DOI: 10.1111/ipd.12610. Available from: http://www.ncbi.nlm.nih.gov/pubmed/31854043.
Letieri A, dos S, Freitas-Fernandes LB, et al. Longitudinal evaluation of salivary Iga-S in children with early childhood caries before and after restorative treatment. J Clin Pediatr Dent [Internet] 2019 43(4):239–243. Available from http://jocpd.org/doi/10.17796/1053-4625-43.4.3.
Kirstilä V, Häkkinen P, Jentsch H, et al. Longitudinal analysis of the association of human salivary antimicrobial agents with caries increment and cariogenic micro-organisms: a two-year cohort study. J Dent Res [Internet] 1998;77(1):73–80. Available from: http://journals.sagepub.com/doi/10.1177/00220345980770011101.
Muthu M, Amarlal D, Prabhu VR, et al. Quantitative assessment of IgA levels in the unstimulated whole saliva of caries-free and caries-active children. J Indian Soc Pedod Prev Dent [Internet] 2008;26(4):158. DOI: 10.4103/0970-4388.44031. Available from http://www.ncbi.nlm.nih.gov/pubmed/19008624.
Sundaresan C, Mathai V, Khosla E, et al. A comparative study of salivary buffering capacity, flow rate, resting pH, and salivary immunoglobulin A in children with rampant caries and caries-resistant children. J Indian Soc Pedod Prev Dent [Internet] 2013;31(2):69. DOI: 10.4103/0970-4388.115697. Available from http://www.ncbi.nlm.nih.gov/pubmed/23886715.
Childers NK, Greenleaf C, Li F, et al. Effect of age on immunoglobulin A subclass distribution in human parotid saliva. Oral Microbiol Immunol [Internet] 2003;18(5):298–301. DOI: 10.1034/j.1399-302x.2003.00084.x. Available from http://www.ncbi.nlm.nih.gov/pubmed/12930521.
Kirtaniya B, Chawla H, Tiwari A, et al. Natural prevalence of antibody titers to glucostltransferase of Streptococcus mutans in serum in high and low caries active children. J Indian Soc Pedod Prev Dent [Internet] 2010;28(2):91. DOI: 10.4103/0970-4388.66744. Available from http://www.ncbi.nlm.nih.gov/pubmed/20660974.
Thaweboon S, Thaweboon B, Nakornchai S, et al. Salivary secretory IgA, pH, flow rates, mutans streptococci and Candida in children with rampant caries. Southeast Asian J Trop Med Public Health [Internet] 2008 39(5):893–899. Available from http://www.ncbi.nlm.nih.gov/pubmed/19058586.