The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 19 , ISSUE 3 ( March, 2018 ) > List of Articles


Evaluation of the Efficacy of Different Mixing Techniques and Disinfection on Microbial Colonization of Polyether Impression Materials: A Comparative Study

Rajni Sharma, Renu B Pachar, Youginder Singla, Sangeeta Poriya, Aalok Mishra, Anshu Garg

Citation Information : Sharma R, Pachar RB, Singla Y, Poriya S, Mishra A, Garg A. Evaluation of the Efficacy of Different Mixing Techniques and Disinfection on Microbial Colonization of Polyether Impression Materials: A Comparative Study. J Contemp Dent Pract 2018; 19 (3):296-300.

DOI: 10.5005/jp-journals-10024-2256

License: CC BY 3.0

Published Online: 01-07-2014

Copyright Statement:  Copyright © 2018; The Author(s).



This study aims to determine the role of mixing techniques of polyether impression materials and efficacy of disinfection on microbial colonization of these impression materials.

Materials and methods

Polyether impression material was mixed using two methods: First by hand mixing (group I) and second using an automixer (group II) with a total of 100 samples. Four microbial strains were studied, which included Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. After incubation, the bacterial colonies were counted, and then, disinfectant solution was applied. The effect of disinfection solution was evaluated for each specimen.


The surface of polyether impression materials mixed with an automixer has less number of voids and overall a smoother surface as compared with the hand-mixed ones. On comparing the disinfection procedures, i.e., specimens without any disinfection and specimens after disinfection, statistically highly significant difference was seen between all the groups.


We can conclude that impression mixing procedures are important in determining the surface characteristics of the impression and ultimately the colonization of bacteria and also determine the importance of disinfection on microbial colonization.

Clinical significance

This study emphasises the deleterious role of nosocomial infections and specific measures that should be taken regarding the prevention of such diseases. Dental impressions are proved to be a source of such infections and may lead to transmission of such diseases. Thus, proper measures should be taken right from the first step of impression taking to minimizing and preventing such kind of contaminations in clinical practice.

How to cite this article

Singla Y, Pachar RB, Poriya S, Mishra A, Sharma R, Garg A. Evaluation of the Efficacy of Different Mixing Techniques and Disinfection on Microbial Colonization of Polyether Impression Materials: A Comparative Study. J Contemp Dent Pract 2018;19(3):296-300.

  1. Antimicrobial activities of dental impression materials. Dent Mater 1998 Nov;14(6):399-404.
  2. Bactericidal effect of a disinfectant dental stone on irreversible hydrocolloid impressions and stone casts. J Prosthet Dent 1989 Nov;62(5):605-607.
  3. The presence and identification of organisms transmitted to dental laboratories. J Prosthet Dent 1990 Aug;64(2):235-237.
  4. ; Hobo, S.; Whitsett, LD.; Jacobi, R.; Brackett, SE. Fundamentals of fixed prosthodontics. 3rd ed. Canada: Quintessence Publisher; 1997. pp. 302-305.
  5. Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: assessment of antimicrobial efficacy. Rev Odonto 2010 Apr;25(2):182-187.
  6. Antimicrobial effect of 4 disinfectants on alginate, polyether, and polyvinyl siloxane impression materials. Int J Prosthodont 2007 May-Jun;20(3):299-307.
  7. Cross infection control of impressions: a questionnaire survey of practice among private dentists in Hong Kong. Hong Kong Dent J 2006 Jan;3:89-93.
  8. Clinical evaluation of the efficacy of removing microorganisms to disinfect patient-derived dental impressions. Int J Prosthodont 2008 Nov-Dec;21(6):531-538.
  9. Effect of mixing technique on surface characteristics of impression materials. J Prosthet Dent 1998 May;79(5):495-502.
  10. The effect of immersion disinfection procedures on dimensional stability of two elastomeric impression materials. J Oral Sci 2008 Dec;50(4):441-446.
  11. Carriage of oral flora on irreversible hydrocolloid and elastomeric impression materials. J Prosthet Dent 1991 Feb;65(2):244-249.
  12. Effect of mixing techniques on bacterial attachment and disinfection time of polyether impression material. Eur J Dent 2013 Sep;7(Suppl 1):S54-S59.
  13. Br Dent J 2007 Jan;202(1):E1, disussion 36-37.
  14. Evaluation of the clinical efficacy of quaternary ammonium components (QAC) as surface disinfectant. J Dent Tehran Univ Med Sci 2006 Jul;3(4):190-194.
  15. Hair follicles as a niche of Staphylococcus aureus in the nose; is a more effective decolonisation strategy needed? J Hosp Infect 2010 Nov;76(3):211-214.
  16. Microbiological evaluation of the efficacy of two new biodetergents on multidrug resistant nosocomial pathogens. Ann Clin Microbiol Antimicrob 2009 Dec;8:35.
  17. Bacterial contamination of stethoscopes used by health workers: public health implications. J Infect Dev Ctries 2010 Aug;4(7):436-441.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.