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VOLUME 19 , ISSUE 7 ( 2018 ) > List of Articles

ORIGINAL RESEARCH

Comparative Evaluation of Cytotoxicity of Root Canal Sealers on Cultured Human Periodontal Fibroblasts: In vitro Study

Pritesh Jagtap, Arpita Agarwalla, Parag Wani, Karan Bhargava, Santosh Martande

Keywords : 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Cytotoxic, Periodontal ligament fibroblasts, Root canal sealer

Citation Information : Jagtap P, Agarwalla A, Wani P, Bhargava K, Martande S. Comparative Evaluation of Cytotoxicity of Root Canal Sealers on Cultured Human Periodontal Fibroblasts: In vitro Study. J Contemp Dent Pract 2018; 19 (7):847-852.

DOI: 10.5005/jp-journals-10024-2346

License: CC BY-NC 3.0

Published Online: 01-03-2018

Copyright Statement:  Copyright © 2018; The Author(s).


Abstract

Aim: To evaluate and compare the cytotoxic effects of different types of root canal. Materials and methods: The sealers were eluted with culture medium for 1 hour, 7 days, and 14 days. Cell viability was estimated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and trypan blue exclusion method on human periodontal ligament (PDL) fibroblast cells. Sealers used are mineral trioxide aggregate (MTA)-based sealer (MTA Fillapex, Angelus), calcium hydroxide-based sealer (Apexit Plus, Ivoclar Vivadent), resin-based sealer (AH Plus, Dentsply), and zinc oxide eugenol-based sealer (Tubli Seal, SybronEndo). Results: The order of cytotoxicity through MTT assay, at the end of the second week, was observed as MTA Fillapex> Tubli Seal> Apexit Plus > AH Plus. The percentage cell viability obtained after trypan blue exclusion method decreased in the order of Apexit Plus> Tubli Seal> AH Plus> MTA Fillapex, which was similar to the reported cytotoxicity from the MTT assay after 1 hour. Conclusion: Each type of sealer showed moderate-to-severe cytotoxic response when compared with the control. The MTA Fillapex was found to be the most cytotoxic sealer. Use of resin-based material as a root canal sealer may result in a more favorable response to PDL fibroblasts. Clinical significance: Having knowledge of the cytotoxicity of various sealers will help in increasing patient's comfort.

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