The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 19 , ISSUE 8 ( 2018 ) > List of Articles

ORIGINAL RESEARCH

Effect of Radiotherapy on Cariogenic Organism Streptococcus sobrinus in Saliva in Head and Neck Cancer: A Clinical Study

Renu Batra, Sonali Kapoor, Shilpi R Daveshwar, Meena R Daveshwar, Vineet Agrawal

Keywords : Dental caries, Oral cancer, Polymerase chain reaction.

Citation Information : Batra R, Kapoor S, Daveshwar SR, Daveshwar MR, Agrawal V. Effect of Radiotherapy on Cariogenic Organism Streptococcus sobrinus in Saliva in Head and Neck Cancer: A Clinical Study. J Contemp Dent Pract 2018; 19 (8):929-932.

DOI: 10.5005/jp-journals-10024-2359

License: CC BY-NC 3.0

Published Online: 01-03-2018

Copyright Statement:  Copyright © 2018; The Author(s).


Abstract

Aim: Aim of the study was to assess salivary Streptococcus sobrinus in head and neck cancer using quantitative polymerase chain reaction (PCR). Materials and methods: Unstimulated saliva samples were collected from head and neck cancer patient preradiotherapy. Unstimulated saliva samples were collected from oral and laryngeal cancer patients after 6 weeks of radiotherapy (dose 60 Gy). The subjects were explained not to consume solids or liquids or carry out any dental hygiene activity 1 hour prior to saliva collection. Accumulated unstimulated saliva was collected in cylindrical tube through funnel. The collected saliva was then transferred to Eppendorf tube containing Tris–ethylenediaminetetraacetic acid (EDTA) (TE) buffer and was transported to lab for real-time PCR analysis. Results: Streptococcus sobrinus significantly increased postradiotherapy as compared with preradiotherapy in head and neck cancer patients. Conclusion: Within the limitation of this study, we conclude that amount of S. sobrinus increases postradiotherapy in head and neck cancer patients. Clinical significance: As radiation therapy has harmful effects on hard and soft tissues of oral cavity, dentists should provide motivation for oral health care to the patients.


PDF Share
  1. Nagler RM. The enigmatic mechanism of irradiationinduced damage to the major salivary glands. Oral Dis 2002 May;8(3):141-146.
  2. Walker MP, Wichman B, Cheng AL, Coster J, Williams KB. Impact of radiotherapy dose on dentition breakdown in head and neck cancer patients. Pract Radiat Oncol 2011;1(3):142-148.
  3. Tong HC, Gao XJ, Dong XZ. Non-mutans streptococci in patients receiving radiotherapy in the head and neck area. Caries Res 2003 Jul-Aug;37(4):261-266.
  4. van Houte J. Role of micro-organisms in caries etiology. J Dent Res 1994 Mar;73(3):672-681.
  5. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986 Dec;50(4):353-380.
  6. Beighton D. The complex oral microflora of high-risk individuals and groups and its role in the caries process. Community Dent Oral Epidemiol 2005 Aug;33(4):248-255.
  7. Leung WK, Jin LJ, Samaranayake LP, Chiu GK. Subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation. Oral Microbiol Immunol 1998 Feb;13(1):1-10.
  8. Valones MA, Guimarães RL, Brandão LA, de Souza PR, de Albuquerque Tavares Carvalho A, Crovela S. Principles and applications of polymerase chain reaction in medical diagnostic fields: a review. Braz J Microbiol 2009 Jan;40(1):1-11.
  9. D'Amario M, Barone A, Marzo G, Giannoni M. Caries-risk assessment: the role of salivary tests. Minerva Stomatol 2006 Jul-Aug;55(7-8):449-463.
  10. Selwitz RH, Ismail AI, Pitts NB. Dental caries. Lancet 2007 Jan;369(9555):51-59.
  11. Kang MS, Oh JS, Kim HJ, Kim HN, Lee IK, Choi HR, Kim OJ, Ko YJ, Lim WB, Park HJ, et al. Prevalence of oral microbes in the saliva of oncological patients. J Bacteriol Virol 2009 Dec;39(4):277-285.
  12. Okada M, Soda Y, Hayashi F, Doi T, Suzuki J, Miura K, Kozai K. PCR detection of Streptococcus mutans and S. sobrinus in dental plaque samples from Japanese pre-school children. J Med Microbiol 2002 May;51(5):443-447.
  13. Homer KA, Patel R, Beighton D. Effects of N-acetylglucosamine on carbohydrate fermentation by Streptococcus mutans NCTC 10449 and Streptococcus sobrinus SL-1. Infect Immun 1993 Jan;61(1):295-302.
  14. Bretz WA, Krahn DD, Drury M, Schork N, Loesche WJ. Effects of fluoxetine on the oral environment of bulimics. Oral Microbiol Immunol 1993 Feb;8(1):62-64.
  15. Lindquist B, Emilson CG. Distribution and prevalence of mutans streptococci in the human dentition. J Dent Res 1990 May;69(5):1160-1166.
  16. Emilson CG. Prevalence of Streptococcus mutans with different colonial morphologies in human plaque and saliva. Scand J Dent Res 1983 Feb;91(1):26-32.
  17. Zhang J, Liu H, Liang X, Zhang M, Wang R, Peng G, Li J. Investigation of salivary function and oral microbiota of radiation caries-free people with nasopharyngeal carcinoma. PLoS One 2015 Apr;10(4):e0123137.
  18. Hu YJ, Wang Q, Jiang YT, Ma R, Xia WW, Tang ZS, Zheng L, Jing PL, Huang ZW. Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing. Int J Oral Sci 2013 Mar;5(1):21-25.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.