Aim: This study compared the tissue reaction of 80 wt% of White Portland cement (WPC) mixed with 20 wt% of three radiopacifying agents: Bismuth oxide/Iodoform/Zirconium oxide with MTA in rat subcutaneous connective tissue.

Materials and methods: The study was performed in 18 albino rats by implanting the WPC mixed with radiopacifying agents loaded in a polyethylene tube. Empty tubes were used as a control. At the end of 7, 30 and 60 days excisional biopsy of the implant along with surrounding tissues was done and sent for histological examination.

Results: In the 7 days experimental period there was no significant difference between groups in terms of the tissue response. In 30 and 60 days period significant difference was seen between the control (empty tube) and the other groups. But there was no significant difference between WPC mixed with radiopacifiers BiO/Iodoform/ZrO₂ and MTA.

Conclusion: The tissue reaction of the tested materials, White Portland cement (WPC) + Bismuth oxide, WPC + Iodoform, and WPC + Zirconium dioxide were similar to MTA (Pro Root MTA) in all experimental periods 7 days, 30 days and 60 days.