The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 19 , ISSUE 11 ( November, 2018 ) > List of Articles

ORIGINAL ARTICLE

Assessment of Enamel Surface Microhardness with different Fluoride Varnishes–An In Vitro Study

Roshan Uthappa, Civy V Pulayath, Siraj P Ismail, Shabna Moyin, Sameer Punathil, Shashit S Bavabeedu

Keywords : Demineralization, Fluoride varnish, Remineralization, Surface microhardness

Citation Information : Uthappa R, Pulayath CV, Ismail SP, Moyin S, Punathil S, Bavabeedu SS. Assessment of Enamel Surface Microhardness with different Fluoride Varnishes–An In Vitro Study. J Contemp Dent Pract 2018; 19 (11):1317-1321.

DOI: 10.5005/jp-journals-10024-2425

License: CC BY-NC 4.0

Published Online: 01-10-2018

Copyright Statement:  Copyright © 2018; The Author(s).


Abstract

Aim: This study aimed to assess the microhardness of the enamel surface after fluoride varnish application. Materials and methods: Thymol of 0.1% in distilled water was used to store the collected healthy sixty teeth. The samples were divided into three groups randomly as per the different application of fluoride varnish. Group A: Fluor protector varnish (FIV) application, group B: Duraphat varnish application and group C: Bifluorid 10 varnish application. The present study followed the pH cycling protocol. Microhardness tester was used to test the microhardness of enamel surface and was expressed as microhardness measurements of Vickers hardness number (VHN) which was performed at baseline, on the 3rd day andon 7th day. Results: At baseline, group A samples mean SMH value was 230.64 ± 12.32 which was slightly more than group B with 229.45 ± 10.22 and group C with 230.10 ± 11.45. There was no significant difference showed with the analysis of variance between the groups. On the 3rd day, there was a slight increase in the mean SMH in group A with 235.39 ± 6.44 and no significant difference between the groups was seen statistically. On the 7th day, the group A showed high SMH value of 262.20 ± 4.89 compared to other groups which didn't show a significantly high statistical difference. Conclusion: On conclusion, post-application of fluorprotector varnish showed higher enamel surface microhardness compared to Duraphat and Bifluorid 10 varnishes. Clinical significance: In young children, fluoride varnishes are effectively used as a noninvasive, anti-caries agent in the treatment of initial caries. Therefore, in routine dental practice, the knowledge about different fluoride varnishes is of importance.


PDF Share
  1. Johansson I, Holgerson PL, Kressin NR, Nunn ME, Tanner AC. Snacking habits and caries in young children. Caries Res. 2010;44:421-430.
  2. Rao SK, Bhat GS, Aradhya S, Devi A, Bhat M. Study of the efficacy of toothpaste containing casein phosphopeptide in the prevention of dental caries: a randomized controlled trial in 12- to 15-year-old high caries risk children in Bangalore, India. Caries Res. 2009;43:430-435.
  3. Tajer DM and Al-Obaidi WA. Dental caries and salivary Interleukin-6 among preterm postpartum women in relation to baby birth weight (Comparative study). J Bagh College Dentistry 2013;25(Special Issue 1):174-177.
  4. Zero DT, Fontana M, Martinez-Mier EA, Ferreira-Zandona A, Ando M, Gonzalez-Cabezas C, and Bayne S. The biology, prevention, diagnosis and treatment of dental caries Scientific advances in the United States. JADA.2009; 140(9 suppl):25S-34S.
  5. Doddamani GM, Girish Babu KL. Effect of Fluoridated Varnishes on surface micro-hardness of Enamel. Int J Oral Health Med Res 2017;4(2):1-3.
  6. Delbem ACB, Bergamaschi M, Sassaki KT, Cunha RF. Effect of fluoridated varnish and silver diamine fluoride solution on enamel demineralization: pH-cycling study. J Appl Oral Sci. 2006;14(2):88-92.
  7. Beltran-Aguilar ED, Goldstein JW, Lockwood SA. Fluoride varnishes. A review of their clinical use, cariostatic mechanism, efficacy and safety. J Am Dent Assoc. 2000;131(5):589-596.
  8. Lata S, Varghese NO, Varughese JM. Remineralization potential of fluoride and amorphous calcium phosphate-casein phospho peptide on enamel lesions: An in vitro comparative evaluation. J Conserv Dent. 2010;13(1):42-46.
  9. Strohmenger L, Brambilla E. The use of fluoride varnishes in the prevention of dental caries: A short review. Oral Dis 2001;7:71.80.
  10. Majithia U, Venkataraghavan K, Choudhary P, Trivedi K, Shah S, Virda M. Comparative evaluation of application of different fluoride varnishes on artificial early enamel lesion: An in vitro study. Indian J Dent Res 2016;27:521-527.
  11. Casals E, Boukpessi T, McQueen CM, Eversole SL, Faller RV. Anticaries potential of commercial dentifrices as determined by fluoridation and remineralization efficiency. J Contemp Dent Pract. 2007;8(7):1-10.
  12. Molaasadolah F, Eskandarion S, Ehsani A, Sanginan M. In Vitro Evaluation of Enamel Microhardness after Application of Two Types of Fluoride Varnish. J Clin Diagn Res. 2017;11(8): ZC64.ZC66.
  13. Subramaniam P, Telegeti S. Effect of different concentrations of fluoride varnish on enamel surface microhardness: An in vitro randomized controlled study. J Indian Assoc Public Health Dent 2016;14:344-347.
  14. Featherstone JDB, O Reilly MM, Shariati M, Brugler S. Enhancement of remineralization in vitro and in vivo. In: Leach SA. Factors affecting de- and remineralization of the teeth. Oxford: IRL Press; 1986:23-34.
  15. Ten Cate JM, Duijsters PPE. Alternating demineralization and remineralization of artificial enamel lesions. Caries Res 1982;16:201-210.
  16. Munshi AK, Reddy NN, Shetty V. A comparative evaluation of three fluoride varnishes: An in vitro study. J Indian Soc Pedod Prev Dent 2001;19:92.102.
  17. Pinar Erdem A, Sepet E, Kulekci G, Trosola SC, Guven Y. Effects of two fluoride varnishes and one fluoride/chlorhexidine varnish on Streptococcus mutans and Streptococcus sobrinus biofilm formation in vitro. Int J Med Sci 2012;9:129.136.
  18. Attin T, Hartmann O, Hilgers RD, Hellwig E. Fluoride retention of incipient enamel lesions after treatment with a calcium fluoride varnish in vivo. Arch Oral Biol. 1995;40:169-174.
  19. Delbem AC, Brighenti FL, Oliveira FA, Pessan JP, Buzalaf MA, Sassaki KT. In vitro assessment of an experimental coat applied over fluoride varnishes. Journal of Applied Oral Science. 2009 Aug;17(4):280-283.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.