Citation Information :
Vanka A, Vishwakarma SK, Bhat MK, Vanka S, Khan AA. Osteo/odontogenic Differentiation of Human Mesenchymal Stem Cells with Platelet-rich Plasma and Mineral Trioxide Aggregate. J Contemp Dent Pract 2019; 20 (10):1171-1178.
Aim: Aim of the study was to investigate the effect of PRP and MTA individually and combined on in vitro human bone marrow mesenchymal stem cells’ (MSCs) proliferation and osteo/odontogenic differentiation potential.
Materials and methods: MSCs were cultured in vitro with MTA, 5% PRP, 10% PRP, MTA with 5%PRP, and MTA with 10% PRP. Fetal calf serum (FCS) was used as control. Cell viability and proliferative efficiency were tested with cell adhesion and MTT assay. Osteo/odontogenic differentiation was assessed and quantified with alizarin red staining.
Results: MTA alone, MTA with 5% PRP, and MTA with 10% PRP showed significantly high proliferation at day 7 and 14 when compared to the control group. Enhanced differentiation and the highest calcium deposition was observed in MTA with the 10% PRP group.
Conclusion: Within limitations of the in vitro environment, results imply an increased proliferation and induction of MSCs into osteo/odontogenic differentiation by the combination rather than a mere sealing of PRP by MTA.
Clinical significance: PRP and MTA have the potential for true regeneration of the pulp tissue. Moreover, the combination of PRP and MTA can be utilized to expand the MSCs to generate adequate numbers for clinical applications, without xenogenic contamination.
Murray PE, Garcia-Godoy F, Hargreaves KM. Regenerative endodontics: a review of current status and a call for action. J Endod 2007;33:377–390. DOI: 10.1016/j.joen.2006.09.013.
Thibodeau B, Trope M. Pulp revascularization of a necrotic infected immature permanent tooth: case report and review of the literature. Pediatr Dent 2007;1(29):47–50.
Shah N, Logani A, Bhaskar U, et al. Efficacy of revascularization to induce apexification/apexogenesis in infected, nonvital, immature teeth: a pilot clinical study. J Endod 2008;34:919–925. DOI: 10.1016/j.joen.2008.05.001.
Andreasen JO, Bakland LK. Pulp regeneration after non-infected and infected necrosis, what type of tissue do we want? A review. Dent Traumatol 2012;28:13–18. DOI: 10.1111/j.1600-9657.2011.01057.x.
Nosrat A, Homayounfar N, Oloomi K. Drawbacks and unfavorable outcomes of regenerative endodontic treatments of necrotic immature teeth: a literature review and report of a case. J Endod 2012;38:1428–1434. DOI: 10.1016/j.joen.2012.06.025.
Schipani E, Kronenberg HM. Adult mesenchymal stem cells-Stem Book. Cambridge (MA): Harvard Stem Cell Institute; 2008–2009 Jan 31.
Baksh D, Song L, Tuan RS. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med 2004;8:301–316. DOI: 10.1111/j.1582-4934.2004.tb00320.x.
Spees JL, Gregory CA, Singh H, et al. Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy. Mol Ther 2004;9:747–756. DOI: 10.1016/j.ymthe.2004.02.012.
Hwang YJ, Choi JY. Addition of mesenchymal stem cells to the scaffold of platelet-rich plasma is beneficial for the reduction of the consolidation period in mandibular distraction osteogenesis. J Oral Maxillofac Surg 2010;68:1112–1124. DOI: 10.1016/j.joms.2008.08.038.
Astori G, Amati E, Bambi F, et al. Platelet lysate as a substitute for animal serum for the ex vivo expansion of mesenchymal stem/stromal cells: present and future. Stem Cell Res Ther 2016;7:1–8. DOI: 10.1186/s13287-016-0352-x.
Martínez CE, González SA, Palma V, et al. Platelet-Poor and Platelet-Rich Plasma Stimulate Bone Lineage Differentiation in Periodontal Ligament Stem Cells. J Periodontol 2016;87:e18–e26. DOI: 10.1902/jop.2015.150360.
Martin G, Ricucci D, Gibbs JL, et al. Histological findings of revascularized/revitalized immature permanent molar with apical periodontitis using platelet-rich plasma. J Endod 2013;39:138–144. DOI: 10.1016/j.joen.2012.09.015.
Bezgin T, Yılmaz AD, Celik BN, et al. Concentrated platelet-rich plasma used in root canal revascularization: 2 case reports. Int Endod J 2014;1:41–49. DOI: 10.1111/iej.12144.
Torabinejad M, Faras H. A clinical and histological report of a tooth with an open apex treated with regenerative endodontics using platelet-rich plasma. J Endod 2012;38:864–868. DOI: 10.1016/j.joen.2012.03.006.
Stefopoulos S, Tsatsas DV, Kerezoudis NP, et al. Comparative in vitro study of the sealing efficiency of white vs grey ProRoot mineral trioxide aggregate formulas as apical barriers. Dent Traumatol 2008;24:207–213. DOI: 10.1111/j.1600-9657.2007.00516.x.
Mozayeni MA, Milani AS, Marvasti LA. Cytotoxicity of calcium enriched mixture cement compared with mineral trioxide aggregate and intermediate restorative material. Aust Dent J 2012;38:70–75. DOI: 10.1111/j.1747-4477.2010.00269.x.
D'Antò V, Di Caprio MP, Ametrano G, et al. Effect of mineral trioxide aggregate on mesenchymal stem cells. J Endod 2010;36:1839–1843. DOI: 10.1016/j.joen.2010.08.010.
Bausset O, Giraudo L, Veran J, et al. Formulation and storage of platelet-rich plasma homemade product. Biores Open Access 2012;1:115–123. DOI: 10.1089/biores.2012.0225.
Amit V, Vishwakarma SK, Vanka GS, et al. In vitro proliferation of MSCs using mineral trioxide aggregate: a most recent material for in situ stem cells mobilisation. Int J Adv Res 2014;2:561–567.
Qian Y, Han Q, Chen W, et al. Platelet-rich plasma derived growth factors contribute to stem cell differentiation in musculoskeletal regeneration. Front Chem 2017;5:89. DOI: 10.3389/fchem.2017. 00089.
Dhurat R, Sukesh MS. Principles and methods of preparation of platelet-rich plasma: a review and author's perspective. J Cutan Aesthet Surg 2014;7(4):189. DOI: 10.4103/0974-2077.150734.
Mazzucco L, Balbo V, Cattana E, et al. Not every PRP-gel is born equal. Evaluation of growth factor availability for tissues through four PRP-gel preparations: Fibrinet, Regen PRP-Kit, Plateltex and one manual procedure. Vox Sang 2009;97:110–118. DOI: 10.1111/j.1423-0410.2009.01188.x.
Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leukocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 2009;27:158–167. DOI: 10.1016/j.tibtech.2008.11.009.
Perut F, Filardo G, Mariani E. Preparation method and growth factor content of platelet concentrate influence the osteogenic differentiation of bone marrow stromal cells. Cytotherapy 2013;15:830–839. DOI: 10.1016/j.jcyt.2013.01.220.
Hass R, Kasper C, Böhm S, et al. Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal 2011;9:12. DOI: 10.1186/1478-811X-9-12.
Nombela-Arrieta C, Ritz J, Silberstein LE. The elusive nature and function of mesenchymal stem cells. Nat Rev Mol Cell Biol 2011;12:126. DOI: 10.1038/nrm3049.
Avinash K, Malaippan S, Dooraiswamy JN. Methods of isolation and characterization of stem cells from different regions of oral cavity using markers: a systematic review. Int J Stem Cells 2017;10:12. DOI: 10.15283/ijsc17010.
Vogl M, Fischer J, Jäger M, et al. Can thrombin-activated platelet releasate compensate the age-induced decrease in cell proliferation of MSC? J Orthop Res 2013;11:1786–1795. DOI: 10.1002/jor. 22433.
Mishra A, Tummala P, et al. Buffered platelet-rich plasma enhances mesenchymal stem cell proliferation and chondrogenic differentiation. Tissue Eng Part C Methods 2009;15:431–435. DOI: 10.1089/ten.tec.2008.0534.
Cho HS, Song IH, Park SY, et al. Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells. Korean J Lab Med 2011;31:212–218. DOI: 10.3343/kjlm.2011.31.3.212.
Chen CL, Huang TH, Ding SJ, et al. Comparison of calcium and silicate cement and mineral trioxide aggregate biologic effects and bone markers expression in MG63 cells. J Endod 2009;35:682–685. DOI: 10.1016/j.joen.2009.02.002.
Takita T, Hayashi M, Takeichi O, et al. Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells. Int Endod J 2006;39:415–422. DOI: 10.1111/j.1365-2591.2006.01097.x.
Maeda H, Nakano T, Tomokiyo A, et al. Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells. J Endod 2010;36:647–652. DOI: 10.1016/j.joen.2009.12.024.
Kwon JY, Lim SS, Baek SH, et al. The effect of mineral trioxide aggregate on the production of growth factors and cytokine by human periodontal ligament fibroblasts. J Korean Acad Conserv Dent 2007;32:191–197. DOI: 10.5395/JKACD.2007.32.3.191.
Tavakolinejad S, Khosravi M, Mashkani B, et al. The effect of human platelet-rich plasma on adipose-derived stem cell proliferation and osteogenic differentiation. Iran Biomed J 2014;18:151.