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VOLUME 21 , ISSUE 1 ( January, 2020 ) > List of Articles

ORIGINAL RESEARCH

Efficacy of Erbium, Chromium-doped Yttrium, Scandium, Gallium and Garnet Laser-activated Irrigation Compared with Passive Ultrasonic Irrigation, Conventional Irrigation, and Photodynamic Therapy against Enterococcus faecalis

Saime A Aydin, Tamer Taşdemir, Celal K Buruk, Davut Çelik

Keywords : Er,Cr:YSGG laser, Laser-activated irrigation, Passive ultrasonic irrigation, Photoactivated disinfection, Root canal disinfection

Citation Information : Aydin SA, Taşdemir T, Buruk CK, Çelik D. Efficacy of Erbium, Chromium-doped Yttrium, Scandium, Gallium and Garnet Laser-activated Irrigation Compared with Passive Ultrasonic Irrigation, Conventional Irrigation, and Photodynamic Therapy against Enterococcus faecalis. J Contemp Dent Pract 2020; 21 (1):11-16.

DOI: 10.5005/jp-journals-10024-2727

License: CC BY-NC 4.0

Published Online: 01-01-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: To compare the antimicrobial effects of two different irrigation solutions activated with erbium, chromium-doped yttrium, scandium, gallium and garnet (Er,Cr:YSGG) laser or an ultrasonic system and a photodynamic therapy (PDT) on Enterococcus faecalis (E. faecalis). Materials and methods: The root canals of 72 single-rooted human permanent incisors were prepared with ProTaper Universal rotary instruments and incubated with E. faecalis (ATCC 29212) for 4 weeks. Then the teeth were randomly divided into seven experimental groups with 10 specimens for canal disinfection procedures. Group I, standard needle irrigation (SNI) with 2.5% sodium hypochlorite (NaOCl); group II, SNI with 2% chlorhexidine gluconate (CHX); group III, laser-activated irrigation (LAI) by Er,Cr:YSGG of NaOCl; group IV, LAI of CHX; and group V, passive ultrasonic irrigation (PUI) of NaOCl; group VI, PUI of CHX; group VII, PDT. The remaining two teeth were used as the control group. After the disinfection procedures were completed, the root canals were filled with phosphate-buffered saline and bacterial samples were taken with sterile paper cones. The cultivation was performed on Mueller-Hinton agar (MHA) plates. The live bacteria were calculated by counting the colonies on these plaques. The statistical analysis was performed using Kruskal-Wallis H test and Miller\'s multiple comparison technique. Results: Both LAI and PUI of NaOCl and PUI of CHX were more successful than the PDT on root canal disinfection (p < 0.05). Conclusion: Within the limitation of the present study, the activation of NaOCl solution by Er,Cr:YSGG laser or an ultrasonic system can be useful in the elimination of the E. faecalis from the canal. The PUI of CHX also has similar results. Photodynamic therapy showed a lower performance compared to these methods. Clinical significance: The activation of the sodium hypochlorite with Er,Cr:YSGG laser or PUI may be useful for removal of the E. faecalis biofilm layer in the root canal.


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