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VOLUME 21 , ISSUE 4 ( April, 2020 ) > List of Articles

ORIGINAL RESEARCH

An In-Vitro Assessment of Surface Roughness, Tensile Bond Strength and Antifungal Activity of Grape Seed Extract-modified Soft Liner

Keywords : Antifungal activity, Grape seed extract, Soft liner, Surface roughness, Tensile bond strength

Citation Information : An In-Vitro Assessment of Surface Roughness, Tensile Bond Strength and Antifungal Activity of Grape Seed Extract-modified Soft Liner. J Contemp Dent Pract 2020; 21 (4):353-358.

DOI: 10.5005/jp-journals-10024-2810

License: CC BY-NC 4.0

Published Online: 01-04-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: This study was conducted to evaluate the grape seed extract (GSE)-modified soft liner regarding surface roughness, tensile bond strength to the denture base material, and the antifungal activity. Materials and methods: The GSE powder was blended with the soft liner powder in ratios of 5 and 10% w/w, and three groups were employed: I, control; II, 5% w/w GSE-modified soft liner; III, 10% w/w GSE-modified soft liner. Evaluation parameters included surface roughness, tensile bond strength to the denture base material, and the antifungal activity. Changes in surface topography were evaluated by scanning electron microscopy. The statistical analysis was performed using the one-way ANOVA followed by the Tukey’s test (α = 0.05). Results: The 5% w/w GSE-modified soft liner showed a significant increase in surface roughness, while both ratios (5 and 10% w/w) of the modified-soft liner exhibited significant increase in tensile bond strength and antifungal activity (p < 0.05). Conclusion: The GSE of 10% w/w considerably enhanced the antifungal activity and tensile bond strength of the modified soft liner to the denture base material without compromising its surface roughness. Clinical significance: The 10% w/w GSE-modified soft liner may be a promising formulation with antifungal activity. It could inhibit fungal adherence and development of fungi-induced lesions or exacerbation of existing ones.


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  1. Żmudzki J, Chladek G, Kasperski J. Biomechanical factors related to occlusal load transfer in removable complete dentures. Biomech Model Mechanobiol 2015;14(4):679–691. DOI: 10.1007/s10237-014-0642-0.
  2. Hashem MI. Advances in soft denture liners: an update. J Contemp Dent Pract 2015;16(4):314–318. DOI: 10.5005/jp-journals-10024-1682.
  3. Almas K. The antimicrobial effects of extracts of Azadirachta indica (neem) and Salvadora persica (Arak) chewing sticks. Indian J Dent Res 1999;10(1):23–26.
  4. Akin H, Tugut F, Guney U, et al. Tensile bond strength of silicone-based soft denture liner to two chemically different denture base resins after various surface treatments. Lasers Med Sci 2013;28(1):119–123. DOI: 10.1007/s10103-012-1082-7.
  5. Makila E, Honka O. Clinical study of a heat-cured silicone soft lining material. J Oral Rehabil 1979;6(2):199–204. DOI: 10.1111/j.1365-2842.1979.tb01281.x.
  6. Goll G, Smith DE, Plein JB. The effect of denture cleansers on temporary soft liners. J Prosthet Dent 1983;50(4):466–472. DOI: 10.1016/0022-3913(83)90564-4.
  7. Kang SH, Lee HJ, Hong SH, et al. Influence of surface characteristics on the adhesion of Candida albicans to various denture lining materials. Acta Odontol Scand 2013;71(1):241–248. DOI: 10.3109/00016357.2012.671360.
  8. Urban VM, Seo RS, Giannini M, et al. Superficial distribution and identification of antifungal/antimicrobial agents on a modified tissue conditioner by SEM-EDS microanalysis: a preliminary study. J Prosthodont 2009;18(7):603–610. DOI: 10.1111/j.1532-849X.2009.00479.x.
  9. Urban VM, de Souza RF, Arrais CA, et al. Effect of the association of nystatin with a tissue conditioner on its ultimate tensile strength. J Prosthodont 2006;15(5):295–299. DOI: 10.1111/j.1532-849X.2006.00130.x.
  10. Waters MG, Jagger RG. Mechanical properties of an experimental denture soft lining material. J Dent 1999;27(3):197–202. DOI: 10.1016/S0300-5712(98)00046-3.
  11. Tocci N, D’Auria FD, Simonetti G, et al. A three step culture system to increase the xanthone production and antifungal activity of Hypericum perforatum subsp. Angustifolium in vitro roots. Plant Physiol Biochem 2012;57:54–58. DOI: 10.1016/j.plaphy.2012.04.014.
  12. Mirkarimi M, Eskandarion S, Bargrizan M, et al. Remineralization of artificial caries in primary teeth by grape seed extract: an in vitro study. J Dent Res Dent Clin Dent Prospects 2013;7(4):206–210. DOI: 10.5681/joddd.2013.033.
  13. Fujii H, Sun B, Nishioka H, et al. Evaluation of the safety and toxicity of the oligomerized polyphenol Oligonol. Food Chem Toxicol 2007;45(3):378–387. DOI: 10.1016/j.fct.2006.08.026.
  14. Rao CN, Rao VH, Steinmann B. Bioflavonoid-mediated stabilization of collagen in adjuvant-induced arthritis. Scand J Rheumatol 1983;12(1):39–42. DOI: 10.3109/03009748309102002.
  15. Han B, Jaurequi J, Tang BW, et al. Proanthocyanidin: a natural crosslinking reagent for stabilizing collagen matrices. J Biomed Mater Res A 2003;65(1):118–124. DOI: 10.1002/jbm.a.10460.
  16. Parvati M, Kandwal A, Singh A. Vitis vinifera (grape) seed extract in periodontal health and disease: a review. J Dent Sci Oral Rehabil 2011;2:10–11.
  17. Urban VM, Lima TF, Bueno MG, et al. Effect of the addition of antimicrobial agents on shore a hardness and roughness of soft lining materials. J Prosthodont 2015;24(3):207–214. DOI: 10.1111/jopr.12205.
  18. Mutluay MM, Ruyter IE. Evaluation of adhesion of chair-side hard relining materials to denture base polymers. J Prosthet Dent 2005;94(5):445–452. DOI: 10.1016/j.prosdent.2005.08.011.
  19. Wright PS, Young KA, Parker S, et al. Evaluating the effect of soft lining materials on the growth of yeast. J Prosthet Dent 1998;79(4):404–409. DOI: 10.1016/S0022-3913(98)70153-2.
  20. Zissiz AJ, Polyzoıs GL, Yannıkakıs SA, et al. Roughness of denture materials: a comparative study. Int J Prosthodont 2000;13(2): 136–140.
  21. Addy M. In vitro studies into the use of denture base and soft liner materials as carriers for drugs in the mouth. J Oral Rehabil 1981;8(2):131–142. DOI: 10.1111/j.1365-2842.1981.tb00486.x.
  22. Bueno MG, Sousa EJB, Hotta J, et al. Surface properties of temporary soft liners modified by minimum inhibitory concentrations of antifungals. Braz Dent J 2017;28(2):158–164. DOI: 10.1590/0103-6440201701266.
  23. Huh JB, Lim Y, Youn HI, et al. Effect of denture cleansers on Candida albicans biofilm formation over resilient liners. J Adv Prosthodont 2014;6(2):109–114. DOI: 10.4047/jap.2014.6.2.109.
  24. de Foggi CC, Machado AL, Zamperini CA, et al. Effect of surface roughness on the hydrophobicity of a denture-base acrylic resin and Candida albicans colonization. J Investig Clin Dent 2016;7(2):141–148. DOI: 10.1111/jicd.12125.
  25. Kulkarni RS, Parkhedkar R. The effect of denture base surface pretreatments on bond strengths of two long term resilient liners. J Adv Prosthodont 2011;3(1):16–19. DOI: 10.4047/jap.2011.3.1.16.
  26. Takahashi Y, Chai J, Takahashi T, et al. Bond strength of denture teeth to denture base resins. Int J Prosthodont 2000;13(1):59–65.
  27. Sung HW, Hsu HL, Shih CC, et al. Cross-linking characteristics of biological tissues fixed with monofunctional or multifunctional epoxy compounds. Biomaterials 1996;17(14):1405–1410. DOI: 10.1016/0142-9612(96)87282-6.
  28. Jorge-Herrero E, Fernández P, Turnay J, et al. Influence of different chemical cross-linking treatments on the properties of bovine pericardium and collagen. Biomaterials 1999;20(6):539–545. DOI: 10.1016/S0142-9612(98)90205-8.
  29. Shi J, Yu J, Pohorly JE, et al. Polyphenolics in grape seedsbiochemistry and functionality. J Med Food 2003;6(4):291–299. DOI: 10.1089/109662003772519831.
  30. Chung RWC, Clark RKF, Darvell BW. The bonding of cold-cured acrylic resin to acrylic denture teeth. Aust Dent J 1995;40(4):241–245. DOI: 10.1111/j.1834-7819.1995.tb04804.x.
  31. Huggett R, John G, Jagger RG, et al. Strength of the acrylic denture base tooth bond. Br Dent J 1992;153(5):187–190. DOI: 10.1038/sj.bdj.4804883.
  32. Korkmaz T, Dogan A, Dogan OM, et al. The bond strength of highly cross-linked denture tooth to denture base polymers: a comparative study. J Adhes Dent 2011;13(1):85–92. DOI: 10.3290/j.jad.a18241.
  33. Lazarin AA, Zamperini CA, Vergani CE, et al. Candida albicans adherence to an acrylic resin modified by experimental photopolymerised coatings: an in vitro study. Gerodontology 2014;31(1):25–33. DOI: 10.1111/j.1741-2358.2012.00688.x.
  34. Paiva LCA, Ribeiro RA, Pereira JV, et al. Avaliação clínica e laboratorial do gel da Uncaria tomentosa (Unha de Gato) sobre candidose oral. Rev Bras Farmacogn 2009;19(2a):423–428. DOI: 10.1590/S0102-695X2009000300015.
  35. Simonetti G, Santamaria AR, D’auria FD, et al. Evaluation of anticandida activity of Vitis vinifera. L. Seed extracts obtained from wine and table cultivars. Biomed Res Int 2014;2014:127021. DOI: 10.1155/2014/127021.
  36. Gadelha LMU, Valadas LAR, Fiallos NDM, et al. Evaluation of the Antifungal Effect Vitis vinifera Extract on Candida albicans. J Young Pharm 2018;10(2):164–168. DOI: 10.5530/jyp.2018.10.37.
  37. Lucile Tiemi ABE, Renata Vieira da MOTA, Franco Maria LAJOLO, et al. Compostos fenólicos e capacidade antioxidante de cultivares de uvas Vitis labrusca L. e Vitis vinifera L. Ciênc. Tecnol Aliment 2007;27(2):394–400. DOI: 10.1590/S0101-20612007000200032.
  38. Cao S, Xu W, Zhang N, et al. A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast. PLoS One. 2012; 7(3):e32943. DOI: 10.1371/journal.pone.0032943.
  39. Jung S, Ko BS, Jang HJ, et al. Effects of slightly acidic electrolyzed water ice and grapefruit seed extract ice on shelf life of brown sole (Pleuronectes herzensteini). Food Sci Biotechnol 2018;27(1):261–267. DOI: 10.1007/s10068-017-0198-8.
  40. Eslami H, Babaei H, Mehrbani SP, et al. Evaluation of antifungal effect of grape seed extract (GSE) on Candida glabrata and Candida krusei: in vitro study. Biomedical Research 2017;28(21):9163–9170.
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