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VOLUME 21 , ISSUE 10 ( October, 2020 ) > List of Articles


Does Etching of the Enamel with the Rubbing Technique Promote the Bond Strength of a Universal Adhesive System?

Naji Kharouf, Davide Mancino, Gautier Rapp, Jihed Zghal, Youri Arntz, Youssef Haikel, François Reitzer

Citation Information : Kharouf N, Mancino D, Rapp G, Zghal J, Arntz Y, Haikel Y, Reitzer F. Does Etching of the Enamel with the Rubbing Technique Promote the Bond Strength of a Universal Adhesive System?. J Contemp Dent Pract 2020; 21 (10):1117-1121.

DOI: 10.5005/jp-journals-10024-2962

License: CC BY-NC 4.0

Published Online: 01-03-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Aim: The aim of this in vitro research was to study the effect of etching by phosphoric acid with rubbing technique on the shear bond strength (SBS) of adhesive universal to enamel. Materials and methods: Sixty extracted teeth were obtained. Three application methods (self-etch, etch-and-rinse, and etch-and-rinse with rubbing technique) were performed to bond the enamel surfaces by a universal adhesive. After 24 hours of immersion in water at 37°C, the specimens were prepared for the SBS test. Scanning electron microscopy was performed to observe the adhesive–enamel interfaces. Optical numeric microscope was used to observe the failure style. Statistical analyses were done with one-way analysis of variance test. Results: Statistically significant higher bond strength values were observed for etch-and-rinse mode with rubbing technique (25.98 ± 5.70) MPa then for the etch-and-rinse without rubbing (22.07 ± 5.27) MPa and self-etch modes (9.96 ± 2.98) MPa. Conclusion: Enamel etched by 37% phosphoric acid with rubbing technique for 20 seconds showed an increase in the SBS of the universal adhesive to enamel surfaces. The tags of the adhesive can be presented more efficiently by rubbing the acid before the bonding process, consequently, an optimal interface for the bonding. Clinical significance: According to the results of this in vitro study, the selective enamel etching mode with rubbing technique is advisable when using the universal adhesive, as it significantly increased the bond strength of this adhesive to enamel surfaces. The clinician should etch the enamel using phosphoric acid with rubbing technique for 20 seconds to promote the bond strength of the universal adhesive system.

  1. Hu JC-C, Chun Y-HP, Al Hazzazzi T, et al. Enamel formation and amelogenesis imperfecta. Cells Tissues Organs (Print) 2007;186(1):78–85. DOI: 10.1159/000102683.
  2. Lacruz RS, Habelitz S, Wright JT, et al. Dental enamel formation and implications for oral health and disease. Physiol Rev 2017;97(3):939–993. DOI: 10.1152/physrev.00030.2016.
  3. Peerzada F, Yiu CKY, Hiraishi N, et al. Effect of surface preparation on bond strength of resin luting cements to dentin. Oper Dent 2010;35(6):624–633. DOI: 10.2341/09-379-L.
  4. Grégoire G, Ahmed Y. Evaluation of the enamel etching capacity of six contemporary self-etching adhesives. J Dent 2007;35(5):388–397. DOI: 10.1016/j.jdent.2006.11.003.
  5. Diniz AC, Bandeca MC, Pinheiro LM, et al. Influence of different etching modes on bond strength to enamel using universal adhesive systems. J Contemp Dent Pract 2016;17(10):820–825. DOI: 10.5005/jp-journals-10024-1937.
  6. Van Meerbeek B, Yoshihara K, Yoshida Y, et al. State of the art of self-etch adhesives. Dent Mater 2011;27(1):17–28. DOI: 10.1016/
  7. Van Landuyt KL, Snauwaert J, De Munck J, et al. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials 2007;28(26):3757–3785. DOI: 10.1016/j.biomaterials.2007.04.044.
  8. Suzuki T, Takamizawa T, Barkmeier W, et al. Influence of etching mode on enamel bond durability of universal adhesive systems. Oper Dent 2016;41(5):520–530. DOI: 10.2341/15-347-L.
  9. Bates D, Retief DH, Jamison HC, et al. Effects of acid etch parameters on enamel topography and composite resin--enamel bond strength. Pediatr Dent 1982;4(2):106–110.
  10. Oyarzún A, Rathkamp H, Dreyer E. Immunohistochemical and ultrastructural evaluation of the effects of phosphoric acid etching on dentin proteoglycans. Eur J Oral Sci 2000;108(6):546–554. DOI: 10.1034/j.1600-0722.2000.00912.x.
  11. Yeşilyurt C, Bulucu B. Bond strength of total-etch and self-etch dentin adhesive systems on peripheral and central dentinal tissue: a microtensile bond strength test. J Contemp Dent Pract 2006;7(2):26–36. DOI: 10.5005/jcdp-7-2-26.
  12. Ozer F, Unlü N, Sengun A. Influence of dentinal regions on bond strengths of different adhesive systems. J Oral Rehabil 2003;30(6):659–663. DOI: 10.1046/j.1365-2842.2003.01064.x.
  13. Reicheneder CA, Gedrange T, Lange A, et al. Shear and tensile bond strength comparison of various contemporary orthodontic adhesive systems: an in-vitro study. Am J Orthod Dentofacial Orthop 2009;135(4):422.e1-6. DOI: 10.1016/j.ajodo.2008.07.013discussion 422-423.
  14. Ayar MK, Erdemir F. Bonding performance of universal adhesives to Er,Cr:YSGG laser-irradiated enamel. Microsc Res Tech 2017;80(4):387–393. DOI: 10.1002/jemt.22807.
  15. Sai K, Takamizawa T, Imai A, et al. Influence of application time and etching mode of universal adhesives on enamel adhesion. J Adhes Dent 2018;20(1):65–77.
  16. Stape THS, Wik P, Mutluay MM, et al. Selective dentin etching: a potential method to improve bonding effectiveness of universal adhesives. J Mech Behav Biomed Mater 2018;86:14–22. DOI: 10.1016/j.jmbbm.2018.06.015.
  17. Yıldırım S, Tosun G, Koyutürk AE, et al. Microtensile and microshear bond strength of an antibacterial self-etching system to primary tooth dentin. Eur J Dent 2008;2(1):11–17. DOI: 10.1055/s-0039-1697347.
  18. Wagner A, Wendler M, Petschelt A, et al. Bonding performance of universal adhesives in different etching modes. J Dent 2014;42(7):800–807. DOI: 10.1016/j.jdent.2014.04.012.
  19. Scherrer SS, Cesar PF, Swain MV. Direct comparison of the bond strength results of the different test methods: a critical literature review. Dent Mater 2010;26(2):e78–e93. DOI: 10.1016/
  20. Reis A, Carrilho M, Breschi L, et al. Overview of clinical alternatives to minimize the degradation of the resin-dentin bonds. Oper Dent 2013;38(4):E1–E25. DOI: 10.2341/12-258-LIT.
  21. Kharouf N, Mancino D, Naji-Amrani A, et al. Effectiveness of etching by three acids on the morphological and chemical features of dentin tissue. J Contemp Dent Pract 2019;20(8):915–919. DOI: 10.5005/jp-journals-10024-2626.
  22. Jacobsen T, Söderholm KJ, Garcea I, et al. Calcium leaching from dentin and shear bond strength after etching with phosphoric acid of different concentrations. Eur J Oral Sci 2000;108(3):247–254. DOI: 10.1034/j.1600-0722.2000.108003247.x.
  23. Turner C, Courts FJ, Gombola GG. The removal of phosphoric acid and calcium phosphate precipitates: an analysis of rinse time. Pediatr Dent 1987;9(3):208–211.
  24. Pouyanfar H, Tabaii ES, Aghazadeh S, et al. Microtensile bond strength of composite to enamel using universal adhesive with/without acid etching compared to etch and rinse and self-etch bonding agents. Open Access Maced J Med Sci 2018;6(11):2186–2192. DOI: 10.3889/oamjms.2018.427.
  25. Kharouf N, Rapp G, Mancino D, et al. Effect of etching the coronal dentin with the rubbing technique on the microtensile bond strength of a universal adhesive system. Dent Med Probl 2019;56(4):343–348. DOI: 10.17219/dmp/111697.
  26. Burke FJT, Hussain A, Nolan L, et al. Methods used in dentine bonding tests: an analysis of 102 investigations on bond strength. Eur J Prosthodont Restor Dent 2008;16(4):158–165.
  27. Imai A, Takamizawa T, Sai K, et al. Influence of application method on surface free-energy and bond strength of universal adhesive systems to enamel. Eur J Oral Sci 2017;125(5):385–395. DOI: 10.1111/eos.12361.
  28. Emamieh S, Elahi M, Ghasemi A. Effect of two universal adhesives on microshear bond strength of resin cement to zirconia. Dent Med Probl 2019;56(3):245–249. DOI: 10.17219/dmp/109349.
  29. Caneppele TMF, Torres CRC, Sassaki A, et al. Effects of surface hydration state and application method on the bond strength of self-etching adhesives to cut enamel. J Adhes Dent 2012;14(1):25–30.
  30. do Amaral RC, Stanislawczuk R, Zander-Grande C, et al. Active application improves the bonding performance of self-etch adhesives to dentin. J Dent 2009;37(1):82–90. DOI: 10.1016/j.jdent.2008. 09.010.
  31. Skupien JA, Tathiane LL, Borges MF, et al. Adhesive systems: Considerations about solvents. Int J Odontostomat 2009;3(2): 119–124.
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