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VOLUME 21 , ISSUE 10 ( October, 2020 ) > List of Articles


Comparative Evaluation of Bactericidal Effect of Silver Nanoparticle in Combination with Nd-YAG Laser against Enterococcus faecalis: An In Vitro Study

Nandhini Ambalavanan, Mahendran Kavitha, Srilekha Jayakumar, Aruna Raj, Smitha Nataraj

Citation Information : Ambalavanan N, Kavitha M, Jayakumar S, Raj A, Nataraj S. Comparative Evaluation of Bactericidal Effect of Silver Nanoparticle in Combination with Nd-YAG Laser against Enterococcus faecalis: An In Vitro Study. J Contemp Dent Pract 2020; 21 (10):1141-1145.

DOI: 10.5005/jp-journals-10024-2941

License: CC BY-NC 4.0

Published Online: 08-01-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Aim and objective: To evaluate the bactericidal effect of silver nanoparticles and silver nanoparticles in combination with Nd-YAG laser against Enterococcus faecalis. Materials and methods: A solution containing 100 μg of silver nanoparticles in 1 mL was prepared by adding 5 mL of 104 M solution of AgNO3 with 5 mL of 0.1 M sodium tricitrate. Synthesized silver nanoparticles were characterized using UV-visible spectrophotometer for optical studies and the transmission electron microscopic analysis for determining the size and shape of the nanoparticles. Groups are as follows: group I—silver nanoparticles against E. faecalis, group II—silver nanoparticles in combination with Nd-YAG laser against E. faecalis, group III—control, E. faecalis bacterial culture alone. Optical density was measured periodically at half an hour interval in spectrophotometer in a 96 well plate and statistically analyzed using one-way ANOVA. Results: The optical density and turbidity of groups I and II began to decrease in 2 hours in comparison with the control. There was a significant difference in mean optical density among the three groups after 1½ hours onward. The study also demonstrated the minimal bactericidal concentration (MBC) as 100 μg/mL of Ag nanoparticles with a size of 15 nm were effective against E. faecalis. Conclusion: The study concluded that silver nanoparticles individually and in conjunction with Nd:YAG laser irradiation would be an effective protocol against E. faecalis. Clinical significance: The combined effect of silver nanoparticles and laser disinfection against E. faecalis holds a promising treatment modality for eradicating resistant pathogens and biofilms embedded deep inside the dentinal tubules that are not amenable to conventional disinfection protocols in root canals.

  1. Siqueira JF, Rôças IN, Ricucci D. Biofilms in endodontic infection. Endod Top 2010;22:33–49. DOI: 10.1111/j.1601-1546.2012.00279.x.
  2. Charles HS, Scott AS, Thomas JB, et al. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006;32(2):93–98. DOI: 10.1016/j.joen.2005.10.049.
  3. Nair PNR, Hendry, Cano S. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after “one visit” endodontic treatment. Oral Surg Oral Pathol Oral Radiol Endod 2005;99(2):231–252. DOI: 10.1016/j.tripleo.2004. 10.005.
  4. Jhajharia K, Parolia A, Shetty KV, et al. Biofilm in endodontics: a review. J Int Soc Prev Community Dent 2015;5(1):1–12. DOI: 10.4103/2231-0762.151956.
  5. Noronha VT, Paula AJ, Durán G, et al. Silver nanoparticles in dentistry. Dent Mater 2017;33(10):1110–1126. DOI: 10.1016/
  6. Bapat RA, Chaubal TV, Joshi CP, et al. An overview of application of silver nanoparticles for biomaterials in dentistry. Mater Sci Eng C Mater Biol Appl 2018;91:881–898. DOI: 10.1016/j.msec.2018.05.069.
  7. Agcinaldo SG, Martha SR, George PT, et al. Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers Surg Med 2007;39(1):59–66. DOI: 10.1002/lsm.20415.
  8. Rodrigues CT, de Andrade FB, de Vasconcelos LRSM, et al. Antibacterial properties of silver nanoparticles as a root canal irrigant against enterococcus faecalis biofilm and infected dentinal tubules. Int Endod J 2018;51(8):901–911. DOI: 10.1111/iej.12904.
  9. Fan W, Wu D, Ma T, et al. Ag-loaded mesoporous bioactive glasses against enterococcus faecalis biofilm in root canal of human teeth. Dent Mater J 2015;34(1):54–60. DOI: 10.4012/dmj.2014-104.
  10. García-Contreras R, Argueta-Figueroa L, Mejía-Rubalcava C, et al. Perspectives for the use of silver nanoparticles in dental practice. Int Dent J 2011;61(6):297–301. DOI: 10.1111/j.1875-595X.2011.00072.x.
  11. Halkai KR, Mudda JA, Shivanna V, et al. Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: an in vitro study. J Conserv Dent 2019;22(2):160–163. DOI: 10.4103/JCD.JCD_518_18.
  12. Halkai KR, Halkai R, Mudda JA, et al. Antibiofilm efficacy of biosynthesized silver nanoparticles against endodontic-periodontal pathogens: an in vitro study. J Conserv Dent 2018;21(6):662–666. DOI: 10.4103/JCD.JCD_203_18.
  13. Chandra A, Yadav RK, Shakya VK, et al. Antimicrobial efficacy of silver nanoparticles with and without different antimicrobial agents against enterococcus faecalis and candida albicans. Dent Hypotheses 2017;8(4):94–99. DOI: 10.4103/denthyp.denthyp_17_17.
  14. Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine 2012;8(1):37–45. DOI: 10.1016/j.nano.2011. 05.007.
  15. Wu D, Fan W, Kishen A, et al. Evaluation of the antibacterial efficacy of silver nanoparticles against Enterococcus faecalis biofilm. J Endod 2014;40(2):285–290. DOI: 10.1016/j.joen.2013.08.022.
  16. Du T, Wang Z, Shen Y, et al. Effect of long-term exposure to endodontic disinfecting solutions on young and old Enterococcus faecalis biofilms in dentin canals. J Endod 2014;40(4):509–514. DOI: 10.1016/j.joen.2013.11.026.
  17. Ramskold O, Fong DC, Stronberg T. Thermal effects and antibacterial properties of energy levels required to sterilize stained root canals with an nd:YAG laser. J Endod 1997;23(2):96–100. DOI: 10.1016/S0099-2399(97)80253-1.
  18. Shrivastava S, Bera T, Roy A, et al. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 2007;18(22):1–9. DOI: 10.1088/0957-4484/18/22/225103.
  19. Kushwaha V, Yadav RK, Tikku AP, et al. Comparative evaluation of antibacterial effect of nanoparticles and lasers against endodontic microbiota: an in vitro study. J Clin Exp Dent 2018;10(12):1155–1160. DOI: 10.4317/jced.55076.
  20. Kim JS, Kuk E, Yu KN, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine 2007;3(1):95–101. DOI: 10.1016/j.nano.2006. 12.001.
  21. Afkhami F, Akbari S, Chiniforush N. Entrococcus faecalis elimination in root canals using silver nanoparticles, photodynamic therapy, diode laser, or laser-activated nanoparticles: an in vitro study. J Endod 2017;43(2):279–282. DOI: 10.1016/j.joen.2016.08.029.
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