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VOLUME 21 , ISSUE 11 ( November, 2020 ) > List of Articles


An In Vitro Assessment of Cytotoxicity of Polyvinyl Siloxane, Polyether, and Polyvinyl Ether Silicone on NIH/3T3 Cells

Priyaranjan, Kunal Kumar, Vikas Vaibhav, Shruti Keerthi Thota, Ashfaq Yaqoob, Vaibhav Awinashe, Arshad Jamal Sayed

Citation Information : P, Vaibhav V, Kumar K, Thota SK, Yaqoob A, Awinashe V, Sayed AJ. An In Vitro Assessment of Cytotoxicity of Polyvinyl Siloxane, Polyether, and Polyvinyl Ether Silicone on NIH/3T3 Cells. J Contemp Dent Pract 2020; 21 (11):1262-1265.

DOI: 10.5005/jp-journals-10024-2927

License: CC BY-NC 4.0

Published Online: 01-05-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Aim and objective: Cytotoxicity of polyvinyl siloxane (PVS), polyether (PE), and polyvinyl ether silicone (PVES) on NIH/3T3 cells. Materials and methods: This in vitro study used elastomeric impression materials which were divided into three groups, group I, II, and III with PVES (EXA'lence light body), PVS (Flexceed light body), and PE impression material (Impregum), respectively. A total of 10 specimens were prepared. Dulbecco's modified Eagle's medium was used for growing mouse cell line NIH/3T3. Cytotoxicity level of all elastomers was measured with the test 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay at regular intervals. Results: There was a decline in the survival rate with PVES as found on day 1, PVS and PE showed on 3rd and 7th day. Kruskal–Wallis test showed a significant difference in all groups at various days (p < 0.05). Conclusion: Authors found that PVES showed early cytotoxic signs as compared to PVS and PE. Cell viability for PVS was highest as compared to PVES and PE impression materials. Clinical implication: Cell viability for PVS was highest as compared to PVES and PE impression materials. This information is useful in the selection of impression materials.

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