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VOLUME 22 , ISSUE 2 ( February, 2021 ) > List of Articles

ORIGINAL RESEARCH

In Vitro Evaluation of Osteoblast Response to the Effect of Injectable Platelet-rich Fibrin Coating on Titanium Disks

Rucha Shah, Raison Thomas, Triveni Mavinkote Gowda, Tarun Kumar Amanna Baron, Gayathri Gunjiganur Vemanaradhya, Shivani Bhagat

Keywords : Bone, Dental implants, Osseointegration, Osteoblasts, Platelet-rich fibrin

Citation Information : Shah R, Thomas R, Gowda TM, Baron TK, Vemanaradhya GG, Bhagat S. In Vitro Evaluation of Osteoblast Response to the Effect of Injectable Platelet-rich Fibrin Coating on Titanium Disks. J Contemp Dent Pract 2021; 22 (2):107-110.

DOI: 10.5005/jp-journals-10024-3039

License: CC BY-NC 4.0

Published Online: 01-02-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: Biomimetic implant surface coatings can improve osteoblast response and enhance osseointegration. This study aimed to assess the response of osteoblast-like cell line (MG-63) coating of injectable platelet-rich fibrin on titanium discs. Materials and methods: Injectable-PRF (i-PRF) was prepared by centrifugation of blood at 700 rpm for 3 minutes without any anti-coagulant in i-PRF tubes. Ten commercially pure titanium discs were divided into control groups comprising plain discs, and test group, titanium discs were coated with i-PRF. These were then added to the cultured MG-63 cells. Cell proliferation, alkaline phosphatase production, and mineralization were assessed in both groups at day 1, 7, 14, and 21. Results: The cell proliferation, alkaline phosphatase production, and mineralization increased significantly from day 1 to day 21 in both test and control groups and was significantly higher in the test group than in control group at day 1, 7, 14, and 21 (p <0.001). Conclusions: Coating of titanium discs with i-PRF causes increased proliferation, alkaline phosphatase production, and increased mineralization at day 1, 7, 14, and 21 in MG-63 osteoblast-like cells. Clinical significance: Improved osteoblast proliferation and mineralization demonstrate enhanced activity on the surface of an implant, which in turn may lead to increased bone to implant contact and faster/ and/or enhanced osseointegration.


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