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VOLUME 22 , ISSUE 5 ( May, 2021 ) > List of Articles


Avian Eggshell Slurry as a Dentin Desensitizing Agent: An In Vitro Assessment Using Two Techniques

Aya A El Tahlawy, Dalia A Saba, Nahed G Bakir

Keywords : Dentin hypersensitivity, Dentin permeability, Dentinal tubules occlusion, Eggshell, In vitro study

Citation Information : El Tahlawy AA, Saba DA, Bakir NG. Avian Eggshell Slurry as a Dentin Desensitizing Agent: An In Vitro Assessment Using Two Techniques. J Contemp Dent Pract 2021; 22 (5):532-537.

DOI: 10.5005/jp-journals-10024-3086

License: CC BY-NC 4.0

Published Online: 09-07-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Aim: This study aims to evaluate the efficacy of avian eggshell slurry as a desensitizing agent compared to casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing Tooth Mousse. Materials and methods: Eggshell powder was prepared and characterized using a scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDX). Forty dentin disks were immersed in 6% citric acid for 2 min to simulate hypersensitive dentin (baseline). Disks were then divided into two groups (n = 20) according to treatment received: eggshell slurry and commercially available Tooth Mousse desensitizing agent. Each group was further divided into two subgroups (n = 10). In subgroup 1, dentin discs were immersed in artificial saliva for four weeks whereas, in subgroup 2, discs underwent acid challenge. Environmental scanning electron microscope (ESEM) analysis and hydraulic conductance test were utilized to calculate dentinal tubules occlusion and reduction of dentin permeability percentages, respectively. Statistical analysis was performed using SPSS 22.0 at a significance level p ≤ 0.05. Results: After application of desensitizing agents, the eggshell slurry group showed a significantly higher percentage of dentinal tubules occlusion compared to the Tooth Mousse group, whereas no significant difference existed in the percentage reduction of dentin permeability between both groups. After both immersion protocols, the eggshell slurry significantly occluded more dentinal tubules compared to Tooth Mousse. On the contrary, results of percentage reduction of dentin permeability revealed no significant difference between both subgroups after acid challenge. After artificial saliva immersion, Tooth Mousse showed a significantly higher percentage reduction of dentin permeability compared to the eggshell slurry. Conclusion: Avian eggshell slurry can effectively occlude open dentinal tubules compared to commercially available Tooth Mousse desensitizing agent. Clinical significance: Avian eggshell slurry can be considered a promising material for the treatment of dentin hypersensitivity.

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  1. Hongal S, Torwane NA, Goel P, et al. The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: a methodological SEM study in vitro. Pharmacognosy Res 2014;6(2):113–119. Available from: DOI: 10.4103/0974-8490.129026.
  2. Santiago SL, Pereira JC, Martineli AC. Effect of commercially available and experimental potassium oxalate-based dentin desensitizing agents in dentin permeability: influence of time and filtration system. Braz Dent J 2006;17(4):300–305. DOI: 10.1590/S0103-64402006000400007.
  3. Dundar A, Yavuz T, Orucoglu H, et al. Evaluation of the permeability of five desensitizing agents using computerized fluid filtration. Niger J Clin Pract 2015;18(5):601–606. DOI: 10.4103/1119-3077.158949.
  4. Torwane NA, Hongal S, Goel P, et al. A clinical efficacy of 30% ethenolic extract of Indian propolis and Recaldent™ in management of dentinal hypersensitivity : a comparative randomized clinical trial. Eur J Dent 2013;7(4):461–468. DOI: 10.4103/1305-7456.120675.
  5. Sasikumar S, Vijayaraghavan R. Low temperature synthesis of nanocrystalline hydroxyapatite from egg shells by combustion method. Trends Biomater Artif Organs 2006;19(2):70–73.
  6. Durmuş E, Çelik I, Aydin MF, et al. Evaluation of the biocompatibility and osteoproductive activity of ostrich eggshell powder in experimentally induced calvarial defects in rabbits. J Biomed Mater Res B Appl Biomater 2008;86(1):82–89. DOI: 10.1002/jbm.b.30990.
  7. Salama R, Khashaba M, El Rouby D. Histomorphometric evaluation of a nano-sized eggshell-containing supplement as a natural alloplast : an animal study. Saudi Dent J 2019;31(3):375–381. DOI: 10.1016/j.sdentj.2019.03.011.
  8. Mony B, Rajesh Ebenezar AV, Ghani MF, et al. Effect of chicken egg shell powder solution on early enamel carious lesions: an invitro preliminary study. J Clin Diagn Res 2015;9(3):ZC30–ZC32. DOI: 10.7860/JCDR/2015/11404.5656.
  9. Elsalamony NA, Abdel-hamid DM, Bakir NG. Bioactivity of a nanoeggshell-modified calcium hydroxide dental cement: an in-vitro study. J Dent Med Sci 2018;17(7):70–80. DOI: 10.9790/0853-1707037080
  10. Allam G, El-geleel OA. Evaluating the mechanical properties, and calcium and fluoride release of glass-ionomer cement modified with chicken eggshell powder. Dent J 2018;6(3):40. DOI: 10.3390/dj6030040.
  11. Onwubu SC, Vahed A, Singh S, et al. Reducing the surface roughness of dental acrylic resins by using an eggshell abrasive material. J Prosthet Dent 2017;117(2):310–314. Available from: DOI: 10.1016/j.prosdent.2016.06.024.
  12. Asghar W, Kim Y-T, Ilyas A, et al. Synthesis of nano-textured biocompatible scaffolds from chicken eggshells. Nanotechnology 2012;23(47):475601. Available from: DOI: 10.1088/0957-4484/23/47/475601.
  13. Salah M, Kataia MM, Kataia EM, et al. Evaluation of eggshell powder as an experimental direct pulp capping material. Futur Dent J 2018;4(2):160–164. DOI: 10.1016/j.fdj.2018.05.008.
  14. Hassan TA, Rangari VK, Rana RK, et al. Sonochemical effect on size reduction of CaCO3 nanoparticles derived from waste eggshells. Ultrason Sonochem 2013;20(5):1308–1315. DOI: 10.1016/j.ultsonch.2013.01.016.
  15. Mosaddegh E, Hassankhani A, Pourahmadi S, et al. Ball mill-assisted preparation of nano-CaCO3 as a novel and green catalyst-based eggshell waste: a green approach in the synthesis of pyrano[4,3-b]pyrans. Int J Green Nanotechnol 2013;1:1–5. DOI: 10.1177/1943089213507160.
  16. Uraz A, Gultekin SE, Senguven B, et al. Histologic and histomorphometric assessment of eggshell-derived bone graft substitutes on bone healing in rats. J Clin Exp Dent 2013;5(1):e23–e29. DOI: 10.4317/jced.50968.
  17. Bashir ASM, Manusamy Y. Characterization of raw egg shell powder (ESP) as a good bio-filler. J Eng Res Technol 2015;2(1):56–60.
  18. Thanatvarakorn O, Nakashima S, Sadr A, et al. In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium-phosphate desensitizer. J Biomed Mater Res B Appl Biomater 2013;101(2):303–309. DOI: 10.1002/jbm.b.32840.
  19. Chen CLC, Parolia A, Pau A, et al. Comparative evaluation of the effectiveness of desensitizing agents in dentine tubule occlusion using scanning electron microscopy. Aust Dent J 2015;60(1):65–72. DOI: 10.1111/adj.12275.
  20. Tian L, Peng C, Shi Y, et al. Effect of mesoporous silica nanoparticles on dentinal tubule occlusion: an in vitro study using SEM and image analysis. Dent Mater J 2014;33(1):125–132. Available from: type=abstract. DOI: 10.4012/dmj.2013-215.
  21. Lata S, Varghese NO, Varughese JM. Remineralization potential of fluoride and amorphous calcium phosphate-casein phospho peptide on enamel lesions: an in vitro comparative evaluation. J Conserv Dent 2010;13(1):42–46. Available from: DOI: 10.4103/0972-0707.62634.
  22. Shi S, Wu Q, Xu YT, et al. Long-term in vitro effectiveness of a bioglass desensitizing agent investigated using electrochemical impedance spectroscopy, atomic force microscopy and scanning electron microscopy. Dentistry 2017;7:4. DOI: 10.4172/2161-1122.1000422.
  23. Pathan AB, Bolla N, Kavuri SR, et al. Ability of three desensitizing agents in dentinal tubule obliteration and durability: an in vitro study. J Conserv Dent 2016;19(1):31–36. DOI: 10.4103/0972-0707.173190.
  24. Pashley DH, Galloway SE. The effects of oxalate treatment on the smear layer of ground surfaces of human dentine. Archs oral Biol 1985;30(10):731–737. DOI: 10.1016/0003-9969(85)90185-2.
  25. Pinto SCS, Bandeca MC, Pinheiro MC, et al. Preventive effect of a high fluoride toothpaste and arginine-carbonate toothpaste on dentinal tubules exposure followed by acid challenge: a dentine permeability evaluation. BMC Res Notes 2014;7:385. DOI: 10.1186/1756-0500-7-385.
  26. Thanatvarakorn O, Nakashima S, Sadr A, et al. Effect of a calcium-phosphate based desensitizer on dentin surface characteristics. Dent Mater J 2013;32(4):615–621. Available from: DOI: 10.4012/dmj.2013-073.
  27. Pereira JC, Segala AD, Gillam DG. Effect of desensitizing agents on the hydraulic conductance of human dentin subjected to different surface pre-treatments—an in vitro study. Dent Mater 2005;21(2):129–138. DOI: 10.1016/
  28. Sales-peres SHDC, Carvalho FN de, Marsicano JA, et al. Effect of propolis gel on the in vitro reduction of dentin permeability. J Appl Oral Sci 2011;19(4):318–323. DOI: 10.1590/s1678-77572011005000004.
  29. Davari AR, Ataei E AH. Dentin hypersensitivity: etiology, diagnosis and treatment; a literature review. J Dent Shiraz Univ Med Sci 2013;14(3):136–145. PMCID: PMC3927677 PMID: 24724135
  30. Kunam D, Manimaran S, Sampath V, et al. Evaluation of dentinal tubule occlusion and depth of penetration of nano-hydroxyapatite derived from chicken eggshell powder with and without addition of sodium fluoride: an in vitro study. J Conserv Dent 2016;19(3):239–244. Available from: DOI: 10.4103/0972-0707.181940.
  31. Khoozani NE, Bahrololoom ME, Bagheri R. Modification of a soft drink by adding calcium carbonate nanoparticles to prevent tooth erosion. J Dent Biomater 2014;1(2):38–44. Available from:
  32. Onwubu SC, Mdluli PS, Singh S, et al. A novel application of nano eggshell/titanium dioxide composite on occluding dentine tubules: an in vitro study. Braz Oral Res 2019;33:1–10. DOI: 10.1590/1807-3107bor-2019.vol33.0016.
  33. Machado AC, Rabelo FEM, Silva VM, et al. Effect of in-office desensitizers containing calcium and phosphate on dentin permeability and tubule occlusion. J Dent 2019;(May):1–7. DOI: 10.1016/j.jdent.2019.05.025.
  34. Freire MN, Holanda JNF, Administrativo C. Characterization of avian eggshell waste aiming its use in a ceramic wall tile paste. Cerâmica 2006;52(324):240–244. DOI: 10.1590/S0366-69132006000400004.
  35. Wang Z, Sa Y, Sauro S, et al. Effect of desensitising toothpastes on dentinal tubule occlusion : a dentine permeability measurement and SEM in vitro study. J Dent 2010;38(5):400–410. DOI: 10.1016/j.jdent.2010.01.007.
  36. Lee SY, Kwon HK, Kim BI. Effect of dentinal tubule occlusion by dentifrice containing nano-carbonate apatite. J Oral Rehabil 2008;35(11):847–853. DOI: 10.1111/j.1365-2842.2008.01876.x.
  37. Yu J, Yang H, Li K, et al. A novel application of nanohydroxyapatite/mesoporous silica biocomposite on treating dentin hypersensitivity: an in vitro study. J Dent 2016;50:21–29. DOI: 10.1016/j.jdent.2016.04.005.
  38. Walsh LJ. The effects of GC Tooth Mousse on cervical dentinal sensitivity : a controlled clinical trial. Int Dent SA 2012;12(1):4–12.
  39. Kowalczyk A, Botuliński B, Jaworska M, et al. Evaluation of the product based on Recaldent technology in the treatment of dentin hypersensitivity. Adv Med Sci 2006;51 Suppl 1:40–42.
  40. Leila P, Shirin Z, Mahboubeh M, et al. Comparison the effect of casein phosphopeptide amorphous calcium phosphate and fluoride varnish on dentin hypersensitivity reduction. Casp J Dent Res 2015;4(2):20–26. DOI: 10.22088/cjdr.4.2.20
  41. Zhang Y, Agee K, Pashley DH, et al. The effects of Pain-Free Desensitizer on dentine permeability and tubule occlusion over time, in vitro. J Clin Periodontol 1998;25(11 Pt 1):884–891. DOI: 10.1111/j.1600-051x.1998.tb02386.x.
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