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VOLUME 22 , ISSUE 10 ( October, 2021 ) > List of Articles


Effect of Aging on the Microhardness of Different Resin-based Fluoride-releasing Fissure Sealants: An In Vitro Study

Ayman M Sulimany, Saad S BinSaleh, Eram AlYahya, Rawan Bataweel, Imtinan Alhussain, Ahmed Almahdy

Keywords : Aging, Fissure sealant, Microhardness, Resin-based fluoride-releasing sealants

Citation Information : Sulimany AM, BinSaleh SS, AlYahya E, Bataweel R, Alhussain I, Almahdy A. Effect of Aging on the Microhardness of Different Resin-based Fluoride-releasing Fissure Sealants: An In Vitro Study. J Contemp Dent Pract 2021; 22 (10):1144-1149.

DOI: 10.5005/jp-journals-10024-3153

License: CC BY-NC 4.0

Published Online: 07-02-2022

Copyright Statement:  Copyright © 2021; The Author(s).


Aim: The aim of the present study was to evaluate the effect of aging on the microhardness score [Vickers hardness number (VHN)] of different resin-based fluoride-releasing sealants compared to non-fluoride resin-based sealants. Materials and methods: A total of 48 extracted sound molars that were sectioned mesiodistally were used. In the current study, four types of sealants were compared: Group A, a resin-based non-fluoride-releasing pit and fissure sealant (Eco-S sealant) served as a control. Groups B, C, and D received resin-based fluoride-releasing pit and fissure sealants Helioseal F, Fissurit F, and Embrace™ WetBond, respectively. Subsequently, each group was further divided into immediate and aged subgroups. The samples had been evaluated regarding their microhardness using a pyramidal diamond indenter of a Vickers hardness test at two-time intervals: Immediately and after the aging process through thermocycling. Results: There were no statistically significant differences between mean VHN and material types (p = 0.72). Aging appeared to significantly increase the mean VHN (p = 0.001). The interaction model between material type and time factor showed that the effect of aging differs by the material type, where the VHN of the Embrace™ group increased significantly after aging from 24.33 ± 5.60 to 31.70 ± 3.59 (p = 0.001). Conclusion: While there were no significant differences in the microhardness of commonly used fluoride-releasing fissure sealants, time appears to significantly increase the mean microhardness score (VHN), especially in the Embrace™ group. Clinical significance: Embrace™ WetBond fissure sealant showed a significant improvement in the mean microhardness score (VHN) with time. However, clinical studies with long-term follow-up are needed to confirm our results.

  1. James SL, Abate D, Abate KH, et al. Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet 2018;392(10159):1789–1858. DOI: 10.1016/S0140-6736(18)32279-7.
  2. BaniHani A, Deery C, Toumba J, et al. The impact of dental caries and its treatment by conventional or biological approaches on the oral health-related quality of life of children and carers. Int J Paediatr Dent 2018;28(2):266–276. DOI: 10.1111/ipd.12350.
  3. Souza JGS, Souza SE, Noronha MDS, et al. Impact of untreated dental caries on the daily activities of children. J Public Health Dent 2018;78(3):197–202. DOI: 10.1111/jphd.12259.
  4. Carvalho JC. Caries process on occlusal surfaces: evolving evidence and understanding. Caries Res 2014;48(4):339–346. DOI: 10.1159/000356307.
  5. Mejàre I, Axelsson S, Dahlën Ga, et al. Caries risk assessment. A systematic review. Acta Odontol Scand 2014;72(2):81–91. DOI: 10.3109/00016357.2013.822548.
  6. Naaman R, El-Housseiny AA, Alamoudi N. The use of pit and fissure sealants – a literature review. Dent J (Basel) 2017;5(4):34. DOI: 10.3390/dj5040034.
  7. Horowitz H, Heifetz S, Poulsen S. Retention and effectiveness of a single application of an adhesive sealant in preventing occlusal caries: final report after five years of a study in Kalispell, Montana. J Am Dent Assoc 1977;95(6):1133–1139. DOI: 10.14219/jada.archive.1977.0201.
  8. Lam PP, Sardana D, Lo EC, et al. Fissure sealant in a nutshell. Evidence-based meta-evaluation of sealants’ effectiveness in caries prevention and arrest. J Evid Based Dent Pract 2021;21(3):101587. DOI: 10.1016/j.jebdp.2021.101587.
  9. Charbeneau GT, Dennison JB, Ryge G. A filled pit and fissure sealant: 18-month results. J Am Dent Assoc 1977;95(2):299–306. DOI: 10.14219/jada.archive.1977.0476.
  10. Lam PP, Sardana D, Ekambaram M, et al. Effectiveness of pit and fissure sealants for preventing and arresting occlusal caries in primary molars: a systematic review and meta-analysis. J Evid Based Dent Pract 2020;20(2):101404. DOI: 10.1016/j.jebdp.2020.101404.
  11. Alsabek L, Al-Nerabieah Z, Bshara N, et al. Retention and remineralization effect of moisture tolerant resin-based sealant and glass ionomer sealant on non-cavitated pit and fissure caries: randomized controlled clinical trial. J Dent 2019;86:69-74. DOI: 10.1016/j.jdent.2019.05.027.
  12. Hatirli Hs, Yasa B, Yasa E. Microleakage and penetration depth of different fissure sealant materials after cyclic thermo-mechanic and brushing simulation. Dent Mater J 2018;37(1):15–23. DOI: 10.4012/dmj.2016-234.
  13. Kühnisch J, Bedir A, Lo Y-F, et al. Meta-analysis of the longevity of commonly used pit and fissure sealant materials. Dent Mater 2020;36(5):e158–e168. DOI: 10.1016/
  14. O'Neill D, Nayyar J, Kinirons M, et al. Pit and fissure sealants-an update. J Irish Dent Assoc 2021;67(2):93–97. Available from:
  15. Faria M, Guedes A, Rompante P, et al. Wear pathways of tooth occlusal fissure sealants: an integrative review. Biotribology 2021;27:100190. DOI: 10.1016/j.biotri.2021.100190.
  16. Prabhakar A, Murthy SA, Sugandhan S. Comparative evaluation of the length of resin tags, viscosity and microleakage of pit and fissure sealants – an in vitro scanning electron microscope study. Contemp Clin Dent 2011;2(4):324. DOI: 10.4103/0976-237X.91797.
  17. Beslot-Neveu A, Courson F, Ruse ND. Physico-chemical approach to pit and fissure sealant infiltration and spreading mechanisms. Pediatr Dent 2012;34(3):57E–61E. Available from:
  18. Şişmanoğlu S. Fluoride release of giomer and resin based fissure sealants. Odovtos 2019;21(2):45. DOI: 10.15517/IJDS.V0I0.36860.
  19. Arora T, Arora D, Tripathi A, et al. An in-vitro evaluation of resin infiltration system and conventional pit and fissure sealant on enamel properties in white spot lesions. J Indian Soc Pedod Prev Dent 2019;37(2):133–139. DOI: 10.4103/1319-2442.261345.
  20. Fernandes KS, Chalakkal P, De Ataide IDN, et al. A comparison between three different pit and fissure sealants with regard to marginal integrity. J Conserv Dent 2012;15(2):146. DOI: 10.4103/0972-0707.94588.
  21. Hahnel S, Henrich A, Bürgers R, et al. Investigation of mechanical properties of modern dental composites after artificial aging for one year. Oper Dent 2010;35(4):412–419. DOI: 10.2341/09-337-L.
  22. Borges BCD, Bezerra GVG, Mesquita JA, et al. Effect of irradiation times on the polymerization depth of contemporary fissure sealants with different opacities. Braz Oral Res 2011;25(2):135–142. Available from:
  23. Silikas N, Eliades G, Watts DC. Light intensity effects on resin-composite degree of conversion and shrinkage strain. Dent Mater 2000;16(4):292–296. DOI: 10.1016/s0109-5641(00)00020-8.
  24. Kitchens B, Wells M, Tantbirojn D, et al. Depth of cure of sealants polymerized with high-power light emitting diode curing lights. Int J Paediatr Dent 2015;25(2):79–86. DOI: 10.1111/ipd.12102.
  25. Thunyakitpisal P, Jiemsirilers S, Kashima D, et al. Depth of cure, flexural strength, microhardness, and cytotoxicity of light activated pit and fissure resin-based sealant experimental prototypes. CU Dent J 2016;39:43–52. Available from
  26. Mazaheri R, Pishevar L, Keyhanifard N, et al. Comparing the effect of topical acidulated phosphate fluoride on micro-hardness of two fissure sealants and one flowable composite. Shahid Beheshti Univ Dent J 2014;32(2):103–110. DOI: 10.22037/jds.v32i2.24804.
  27. Diener V, Zinelis S, Eliades T. Water-induced effects on the hardness and modulus of contemporary sealants derived from instrumented indentation testing (IIT). J Contemp Dent Pract 2019;20(6):653–656. Available from:
  28. Szczesio-Wlodarczyk A, Sokolowski J, Kleczewska J, et al. Ageing of dental composites based on methacrylate resins—a criticaleview of the causes and method of assessment. Polymers 2020;12(4):882. DOI: 10.3390/polym12040882.
  29. Izabela M. Barszczewska-Rybarek. Characterization of urethane-dimethacrylate derivatives as alternative monomers for the restorative composite matrix. Dent Mater 2014;30(12):1336–1344. DOI: 10.1016/
  30. Aminoroaya A, Neisiany RE, Khorasani SN, et al. A review of dental composites: challenges, chemistry aspects, filler influences, and future insights. Compos Eng 2021:216. DOI: 10.1016/j.compositesb.2021.108852.
  31. Baheti AS, Bhayya DP, Gupta S, et al. Assessment of clinical success of three sealants: Embrace-Wetbond, Clinpro, and Helioseal-F in permanent molars: an in vivo study. Pediatr Dent 2020;3(1):7–13. DOI: 10.5005/jp-journals-10077-3035.
  32. Panigrahi A, Srilatha K, Panigrahi RG, et al. Microtensile bond strength of embrace wetbond hydrophilic sealant in different moisture contamination: an in-vitro study. J Clin Diagn Res 2015;9(7):ZC23. DOI: 10.7860/JCDR/2015/11662.6178.
  33. Askarizadeh N, Heshmat H, Zangeneh N. One-year clinical success of embrace hydrophilic and Helioseal-F hydrophobic sealants in permanent first molars: a clinical trial. J Dent (Tehran) 2017;14(2):92–99. Available from:
  34. Kim J-W, Jang K-T, Lee S-H, et al. Effect of curing method and curing time on the microhardness and wear of pit and fissure sealants. Dent Mater 2002;18(2):120–127. DOI: 10.1016/s0109-5641(01)00030-6.
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