Citation Information :
Huamaní K, Vilchez L, Jáuregui H. Comparison of the Antifungal Efficacy of Four Concentrations of Minthostachys mollis (Muña) Essential Oil against Candida albicans: An In Vitro Study. J Contemp Dent Pract 2021; 22 (11):1227-1231.
Aim and objective: This paper aims to compare the antifungal efficacy of four concentrations of Minthostachys mollis essential oil (MEO) against Candida albicans ATCC 10231.
Materials and methods: This study was conducted in vitro. Ten kilograms of M. mollis (Muña) were collected in the city of Tarma, Peru. The plant was then dried in the shade at room temperature (21°C), and the essential oil was obtained through distillation. C. albicans ATCC 10231 was cultured at a MacFarland scale of 0.5, which corresponds to a concentration of 3 × 108 CFU/mL. Each plate was filled with one of the four MEO concentrations (25, 50, 75, or 100%), dimethyl sulfoxide (negative control), or fluconazole (positive control), a known antifungal agent. After incubation, each plate was examined using the Kirby-Bauer method.
Results: Compared to MEO 25%, MEO 50%, and MEO 75%, MEO 100% had the highest antifungal efficacy at 24, 48, and 72 hours of evaluation, with an average of 18.9 ± 0.7, 18.2 ± 0.7, and 17.0 ± 0.4 mm, respectively. However, fluconazole had higher efficacy (27.9 ± 0.5, 27.5 ± 0.5, and 23.7 ± 0.7 mm, respectively). Post hoc analysis showed that there were significant differences between all concentrations of the MEO groups and their respective positive and negative control groups (p <0.001).
Conclusion: Among the MEO groups, the pure concentration (MEO 100%) had the highest antifungal efficacy. However, fluconazole presented greater efficacy, and the differences were statistically significant.
Clinical significance: This research allowed to know the efficacy of this natural resource against one of the most prevalent fungi in the oral cavity. Therefore, a line of research could be opened to deepen its potential benefits for oral health.
Lortholary O, Renaudat C, Sitbon K, et al. French Mycosis Study Group. Worrisome trends in incidence and mortality of candidemia in intensive care units (Paris area, 2002–2010). Intensive Care Med 2014;40(9):1303–1312. DOI: 10.1007/s00134-014-3408-3.
Gaitán-Cepeda LA, Sánchez-Vargas O, Castillo N. Prevalence of oral candidiasis in HIV/AIDS children in highly active antiretroviral therapy era. A literature analysis. Int J STD AIDS 2015;26(9):625–632. DOI: 10.1177/0956462414548906.
Odgaard L, Kothari M. Prevalence and association of oral candidiasis with dysphagia in individuals with acquired brain injury. Brain Inj 2018;32(2):247–251. DOI: 10.1080/02699052.2017.1407960.
Mora FD, Araque M, Rojas LB, et al. Chemical composition and in vitro antibacterial activity of the essential oil of Minthostachys mollis (Kunth) Griseb Vaught from the Venezuelan Andes. Nat Prod Commun 2009;4(7):997–1000. DOI: 10.1177/1934578X0900400726.
Rojas-Armas JP, Arroyo-Acevedo JL, Ortiz-Sánchez JM, et al. Corrigendum to “potential toxicity of the essential oil from Minthostachys mollis: a medicinal plant commonly used in the traditional Andean medicine in Peru”. J Toxicol 2020;2020:2103456. DOI: 10.1155/2020/2103456.
Schmidt-Lebuhn AN. Ethnobotany, biochemistry and pharmacology of Minthostachys (Lamiaceae). J Ethnopharmacol 2008;118(3):343–353. DOI: 10.1016/j.jep.2008.05.030.
Linares-Otoya V. Considerations for the use and study of the Peruvian “muña” Minthostachys mollis (Benth.) Griseb and Minthostachys setosa (Briq.) Epling. Ethnobotany Res Appl 2020;19. Available from: http://dx.doi.org/10.32859/era.19.29.1-9.
Castro-Alayo EM, Chávez-Quintana SG, Auquiñivín-Silva EA, et al. Essential oils of native plants from Peru: effect of the place of cultivation on the physicochemical characteristics and antioxidant activity. Scientia Agropecu 2019;10(4):479–487. DOI: 10.17268/sci.agropecu.2019.04.04.
Calderon A, Salas J, Dapello G, et al. Assessment of antibacterial and antifungal properties and in vivo cytotoxicity of Peruvian Passiflora mollisima. J Contemp Dent Pract 2019;20(2):145–151. DOI: 10.5005/jp-journals-10024-2489.
Mayta-Tovalino F, Gamboa E, Sánchez R, et al. Development and formulation of the experimental dentifrice based on Passiflora mollissima (Tumbo) with and without Fluoride Anion: antibacterial activity on seven antimicrobial strains. Int J Dent 2019;2019:9056590. DOI: 10.1155/2019/9056590.
Alegre A, Iannacone J, Carhuapoma M. Toxicity of aqueous, ethanolic and hexanic extracts of Annona muricata, Minthostachys mollis, Lupinus mutabilis, and Chenopodium quinoa against Tetranychus urticae and Chrysoperla externa. Chil J Aric Anim Sci 2017;33(3): 273–284. Available from: https://www.researchgate.net/publication/321768954.
Banchio E, Zygadlo J, Valladares GR. Effects of mechanical wounding on essential oil composition and emission of volatiles from Minthostachys mollis. J Chem Ecol 2005;31(4):719–727. DOI: 10.1007/s10886-005-3540-1.
Lock O, Perez E, Villar M, et al. Bioactive compounds from plants used in Peruvian traditional medicine. Nat Prod Commun 2016;11(3): 315–337. Available from: https://www.researchgate.net/publication/301583788.
Pellegrini MC, Alonso-Salces RM, Umpierrez ML, et al. Chemical composition, antimicrobial activity, and mode of action of essential oils against Paenibacillus larvae, etiological agent of American Foulbrood on Apis mellifera. Chem Biodivers 2017;14(4):1–18. DOI: 10.1002/cbdv.201600382.
Fernández MC, Fárez DLA, Roblez NCC, et al. Chemical composition and antibacterial activity of the essential oil of Minthostachys mollis Griseb against Staphylococcus aureus. Rev Cuba Farm 2017;51(4). Available from: http://www.revfarmacia.sld.cu/index.php/far/article/view/183.
Cano C, Bonilla P, Roque M, et al. In vitro antifungal activity pand metabolites of the essential oil of Minthostachys mollis (muña) leaves. Rev Peru Med Exp Salud Publica 2008;25(3):298–301. Available from: http://www.scielo.org.pe/scielo.php?pid=S1726-46342008000300 008&script=sci_abstract&tlng=en.
Peña D, Gutiérrez M. Antimicrobial effect of the essential oil of Minthostachys mollis on frequent microorganisms in the lower respiratory tract. Rev Cienc Tecnol 2017;13(3):55–66. Available from: https://revistas.unitru.edu.pe/index.php/PGM/article/view/1874.
Torrenegra-Alarcón M, Granados-Conde C, Durán-Lengua M, et al. Composición Química y Actividad Antibacteriana del Aceite Esencial de Minthostachys mollis. Orinoquia 2016;20(1):69–74. DOI: 10.22579/20112629.329.
Alcalá KM, Alvarado AG, Alejandro LA, et al. Actividad antimicótica del aceite esencial de las hojas de Minthostachys mollis (muña) comparado con el fluconazol en cultivo de Candida albicans. Ciencia e Investigación Médico Estudiantil Latinoamericano 2011;16(2):83–86. Available from: https://www.cimel.felsocem.net/index.php/CIMEL/article/view/204.
Poma-Castillo L, Espinoza-Poma M, Mauricio F, et al. Antifungal activity of ethanol-extracted Bixa orellana (L) (Achiote) on Candida albicans, at six different concentrations. J Contemp Dent Pract 2019;20(10):1159–1163. DOI: 10.5005/jp-journals-10024-2672.